43 replies to this topic

[Hippie3]

easy way to get sterile prints =
get UV sterilizer.
cut up rectangles of foil, as many as needed, 1 per print.
place cap on foil to drop spores.
place spore covered foil into small ziplock and seal.
cover the printed area with opaque light shielding
[a foil circle slightly smaller than the print]
turn on UV sterilizer and zap both sides of the foil/baggie.
voila!

[Hippie3]

UV-c Light Wand KF-202
http://www.amazon.co...d_bxgy_k_text_b

[coorsmikey]

This Tek also work on spore prints of questionable sterility. Print that have lying around for while in some dirty drawer or something, Just give them a zap before you use them.

[Hippie3]

i love it... any idea what affect the uv light would have on spores themselves?

it would kill them-
which is why you cover them when you zap.

...
cover the printed area with opaque light shielding
[a foil circle slightly smaller than the print]...

[Phx]

Great to know....I'll be buying one this week some time.

Now I have a question....would it be a good idea to zap the foil before making the print?

[Hippie3]

i just take a pile of foil squares, wrap it in more foil
then bake in oven at 350*F for an hour.
unwrap in the glovebox where do the actual printing.

[Phx]

Ok thanks

[Hippie3]

you can use povidone iodine [ betadine ] to swab upper cap surface clean

[RITTER]

is there any other place to purchase other than "amozon"?

[Hippie3]

uv sterilizer wand
sure, just google it

[sandman]

Nice Hippie! This will make print contams drop dramatically in the OMC if people catch on.

[Hippie3]

easy as pie

[Foster]

Hmmm, wonder how I could incorporate my uvonair into this. Maybe just place the output side of the uvonair in a tub with the foil and let it sit for a while before printing and after.

Nice find/idea Hip:eusa_clap

[Hippie3]

btw
the UV-C wand is also useful on folded foil prints.
you can zap the outside as you unfold it,
sterilizing all exposed surfaces as revealed,
front and back,
until you expose the print-
then pop it in a ziplock, add sterile water to dissolve it
and fill up syringe for dilution / use later.
of course
all sterile work should be done in a glovebox or
in front of a flowhood,
to ensure new contams do not fall onto the foil/print.

[eatyualive]

excellent was just discussing with someone in pm about getting one of these!:headbang:

[waylitjim]

re: UV protection, various sized coins could be used for placing over the print.

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[Hippie3]

yep.
btw
can also be used on agar plates,
cover the transfer/inoculated area
then zap the rest.

i think next i'm going to try
zapping small patches of mold
to see how effective it is...

they sell bigger wands than mine,
for like doing bed sheets,
about $200 for one like 18" long
and a couple inches wide.
thinking maybe 'clean' casing surface until mycelia peeks through ?
dunno, just playing around...

[kcmoxtractor]

Watching it zap a mold would be awesome to watch microscopically! How much does it penetrate, or does it just work on the surface?

[Hippie3]

it's visible light, more or less-
penetration is minimal,
depends on intensity, distance and target material.

[the_chosen_one]

yep.
btw
can also be used on agar plates,
cover the transfer/inoculated area
then zap the rest.

i think next i'm going to try
zapping small patches of mold
to see how effective it is...

they sell bigger wands than mine,
for like doing bed sheets,
about $200 for one like 18" long
and a couple inches wide.
thinking maybe 'clean' casing surface until mycelia peeks through ?
dunno, just playing around...

just hit that agar for a longer period of time. sneaky little bacteria find agar a good shield. treat both top and bottom sides of the dish.

also.. i can't stress enough! eye protection! very important. an 18-36 watt can cause flashburn in less than a second.

:heart: