I was looking for a container that is slightly smaller than the standard 150mm petri dish which was also disposable and easy to use. I found and liberated the following SOLO souffle cups:
This is what the finished plate looks like:
They are very easy to use, store very well (about a year, more depending on how thick the agar is poured), and require no parafilm or other sealant.
So I start out by taking a bunch of new, empty cups into the glovebox and wiping them down inside and out with bleach water/alcohol/peroxide - Any one of those is fine. After wipng out each one, I put the caps on and remove a batch frome the glovebox. This time I used my brand spankin' new schmuvbox (thanks, eatyu)
I take my prepared agar from storage and heat it in a water bath.
And suck it into a big syringe. 10ml syringes can get you 2 plates if you are stingy.
Then I wipe the lid with alcohol and inject the agar.
After I pull the syringe out, I tape over the hole with the smallest peice of scotch tape i can manage to pull off the roll
I bag 'em up and throw them in a drawer for later use. As I said earlier, they are good for a minimum of 6 months when poured very thinly, about a year if I use 12ml or more of agar (the pint jar of prepared agar in the pics is about a year old, unrefrigerated, and I can't tell the difference between it and a batch made yesterday). I also like the fact that I can just shoot spores or lc right thru the 1st hole made in the lid. If anyone can tell me a good coring tek for cloning, I'd love to hear it because I can never get a good tissue sample from the shroom to the needle to the agar succesfully. My goal is to be able to bring these to FOAF's house, core a sample in open air, and then shoot it right thru the lid of these babies.
On a related note, I'm lookng for someone to point me to a supplier of luer lock needles larger than 16ga.
Thanks, and hope you enjoyed the thread!
Edited by coorsmikey, 05 May 2016 - 08:58 PM.