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Agar Poke-a-Plates


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35 replies to this topic

#21 Hippie3

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Posted 29 August 2009 - 01:33 PM

lc doesn't do strain isolation

pure genius, sideshow.
:bow:

#22 masterplain88

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Posted 29 August 2009 - 01:35 PM

yea thats what i thought but then i saw the lc part. thanks for clearing that up hippie3. 08-29-09 00:14SideshowUpdate
3 days later, we have some myc growth from 2 lc's:

Attachment 139604

Attachment 139605

A control (one of the 9 done before)

Attachment 139606

And one that isn't looking well (the only one that has gone south)

Attachment 139607

#23 Sideshow

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Posted 29 August 2009 - 01:38 PM

That means a lot to me Hippie, thank you.:dance:

#24 masterplain88

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Posted 29 August 2009 - 01:40 PM

sorry do be a bother but what is the ratio and mix procedures for your mrya malt agar.? thanks,
im thinking of just buying pr-made agar.
masterplain88

It is MRYA or malt extract, brf, nutritional yeast & agar powder.
I used lc's on the agar to start the culture.
In reference to your lc question, this should do nicely:

http://mycotopia.net...tek-merged.html



#25 Sideshow

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Posted 29 August 2009 - 02:03 PM

sorry do be a bother but what is the ratio and mix procedures for your mrya malt agar.? thanks,
im thinking of just buying pr-made agar.
masterplain88


I would reccommend premixed powder agar if it is your first time. Sporeworks brand PDA is great, for one.

MRYA Recipe: (1/2 pint)
250ml Water
1.5g BRF
3g Malt Extract
.25g Nutritional Yeast
3g Agar

I used to premix the ingredients in boiling water and then pc, but that is a PITA. Nowadays, I just dump everything into a pint mason, shake vigorously for a minute, and pc 30 minutes. Lastly, I also make sure that the agar has not separated (by lightly swirling the jar) as it hardens.

Edited by Sideshow, 29 August 2009 - 02:04 PM.
premixed, not pre-made


#26 masterplain88

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Posted 29 August 2009 - 02:29 PM

thanks bro good write up you got here for some one like me looking to get into isolation work. your a true asset to the topia community. good work man.
peace.
masterplain88

#27 Leftover Salmon

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Posted 29 August 2009 - 02:38 PM

One more newbish question from Salmon,,, Could swim use an isolated chunk of myc growth from agar to make one stellar LC? Or would this be a waste of time? Also could swim drop pieces of agar with myc directly into a grain jar or bag? Thanks you for the knowledge! :bow:
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#28 Sideshow

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Posted 29 August 2009 - 03:14 PM

Could swim use an isolated chunk of myc growth from agar to make one stellar LC?


That's exactly what I do

could swim drop pieces of agar with myc directly into a grain jar or bag?


Yessir

#29 BuckarooBanzai

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Posted 29 August 2009 - 04:45 PM

When agar is well colonized, it is easy to transfer myc with a syringe of sterile water.

Just open the plate enough to stick the needle in and then squirt in 2-3mL of water. The myc is very easy to scrape off the agar with the sterile needle and then you can suck the myc water back up into the syringe.

I really prefer to do myc transfers as LCs or liquid slurry in syringes. Syringes cut contam rates compared to open jars and plates, even with a flow hood.

And you can do syringe work very successfully without a flow hood.

#30 Leftover Salmon

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Posted 29 August 2009 - 09:26 PM

Awesome! Thanks for the tips folk! I, Swim, and FOAF all love this place & the people within.

I am excited to try this agar biz...

#31 bugs

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Posted 29 August 2009 - 10:34 PM

When agar is well colonized, it is easy to transfer myc with a syringe of sterile water.

Just open the plate enough to stick the needle in and then squirt in 2-3mL of water. The myc is very easy to scrape off the agar with the sterile needle and then you can suck the myc water back up into the syringe.

Semms that would work fine in the cuppy things, too. Poke a needle throuhg the lid, drip and sip.

By the way, frickin' ingenious tek. Gotta love that high-grade ghetto. Perfect match for the shmuvbox.
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#32 masterplain88

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Posted 08 September 2009 - 03:32 PM

procedure on the tek would work with bub tweeks like


Semms that would work fine in the cuppy things, too. Poke a needle throuhg the lid, drip and sip.

By the way, frickin' ingenious tek. Gotta love that high-grade ghetto. Perfect match for the shmuvbox.



#33 waterchilde

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Posted 06 November 2009 - 08:28 AM

Hey, found a source for needles that has 14 ga on up and up to 3' needles. Howard electronic instruments is online and I have their ph # , 1-800-394-1984 . now, can you tell me if the nylon vet syringes are autoclavable or is there a way that I can re-sterilize these new syringes over again for multiple useage. looking to learn thanx...

#34 waterchilde

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Posted 06 November 2009 - 08:32 AM

found it! fo all needle supplies, check this out. http://www.howardele...en/needles.html

#35 Indonesia

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Posted 06 November 2009 - 11:19 AM

Nice work sideshow. my foaf has done this tek before with petris..good sound agar technique for a glovebox.

:rasta:

#36 copelandiaKidd

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Posted 06 November 2009 - 12:16 PM

this is awesome!!!! I have been looking for an alternative to half pints for a while now!!!!!




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