Making mycelia slurry syringes - bulk inoculant Tek
Posted 29 April 2010 - 01:15 PM
Also, when you suck the slurry out, an equivalent amount of room air gets pulled back into the jar. I don't know if pulling room air through moist poly would automatically be a contaminant vector.
I strongly suspect poly filters for slurry jars isn't a great idea.
Posted 30 April 2010 - 10:59 AM
G'luck and make sure to let us know how it turns out.
Posted 24 May 2010 - 02:37 PM
Posted 24 May 2010 - 02:55 PM
Posted 26 May 2010 - 07:28 PM
A thick blob on both sides of the lid holds tightly and stands up to a lot of needle punctures while still maintaining a solid seal (with no need for adhesive).
You can make an injection hole even easier to see by outlining it with a sharpie before applying the silicone blob. I have bad eyes and that helps a lot (blue and/or red Sharpie are also excellent).
- ryanzreality likes this
Posted 26 May 2010 - 10:06 PM
Posted 07 June 2010 - 04:52 PM
Posted 24 June 2010 - 06:34 AM
Posted 24 August 2010 - 01:43 AM
Thanks Buck for all your wisdom and advice!!! :bow:
My LC (unpredictable results) days are over...
WE THANK YOU!!!
Question: Would putting some of this in an LC be a good idea to extend it's life? (grow it out for a few days and refrigerate it - just to have a master myc jar to keep on hand?)
Just a thought... :eusa_thin
Posted 11 September 2010 - 03:32 PM
keep y'all posted.
should i really toss the slurry jar?..maybe it would be good for a second round in a few days? guess i shouldnt risk it eh?
Posted 24 January 2011 - 08:37 PM
Howdy Fellow Mycophiles
A recent "mistake" in reading labels turned a batch of PDA into a batch of PDG when Guar gum was used in place of Agar Agar.
After cooking, sterilizing and cooling to 'hot to touch' then pouring into petri dishes, which were covered, cooled and examined to find fluidilty rather than jel. Discovered mistake and with no Agar, inoculations of plates (bowls??) with tissue fragments from fresh and dried mushrooms, edible and others was performed.
Most plates had nothing but contams. But kept 2 500 ml jars 1/2 full and sterilized and kept sealed.
So yesterday had a print and dumped a shot in both of the PDG jars (viscosity of honey, water white, slighly translucent). Put it in sterile, covered airhole and shook spores into PDG which had been charged with air by vigorous shaking moments before.
Today a white foamy mass of bubbles has devloped on the top third of the mass.
A closeup exam show little packets of material suspended in the thick goo and releasing tiny bubbles of gas which constrained by the viscosity leave a trail as they rise upward. Not sure what the gas is. I presume CO2.
Now since I'm as Noobie to mycology as you can get, though well experienced in matters of deep scientific research, I can make no claims as to it's ultimate usefulness, but there are interesting features here.
Experienced mycologists will have wisdom on this.
I will report my own experiences and invite others who may already played such games or who may care to, to offer comments and advice.
Edited by Sidestreet, 02 October 2016 - 03:08 PM.
additional relevant photo
Posted 27 January 2011 - 09:26 PM
Neat picture, by the way. Point sources evolving perfectly round bubbles rising slowly in a thick matrix.
Posted 27 January 2011 - 10:48 PM
The PDG stopped bubbling. no further activity of any kind. Either spores were unviable (methinks), or the environment too "hot" for them to survive.
The spores have done very poorly everywhere, except one 1/2 pint BRF jar, where a few spots are visible after 5 days (Ps. Cy) at 75 - 78F.
I love the "matrix" as a time distorter. The viscosity is such you can barely see the bubbles move. They do enlarge as they rise as they should.
They are cool. I plan experiments with this stuff. I just happened to have a couple sterile jars of the stuff and a newly opened syringe aching for a nice tight opening to slid into (oh shit time to talk to my wife...)
anyways had to try it.
thanks for the rep :thumbup:
Posted 17 February 2011 - 04:35 PM
Now its on :-D This should nearly double if not triple my soon to be colonized grain that is going to be mixed with equal parts of bulk :-D :-D :-D