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P. Semperviva


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#1 kcmoxtractor

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Posted 13 November 2009 - 01:08 AM

This is my first attempt at an exotic species, so bear with me.

They were inoculated on 11-5 and show no growth so far. How long do they normally take to germinate on brf?

I did make a mistake and put just a single silicone port in the center of the jar instead of 4 on the edges. Could that be why I can't see any growth?

I did the standard PF tek, and sterilized my jars for 50 mins at 15 psi. Used 10 of the 4 oz jelly jars, 1 half pint, and 2 pint jars.

Am I correct that nobody has gotten a liquid culture to work with this species? If it has been attempted would you list your recipe?

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#2 the_chosen_one

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Posted 13 November 2009 - 01:14 AM

:eusa_clap

about 5 - 15 days. my average is around 8-10 to see good germination in a four port jar.

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#3 Thre365ive

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Posted 13 November 2009 - 04:22 AM

This is my first attempt at an exotic species, so bear with me.

They were inoculated on 11-5 and show no growth so far. How long do they normally take to germinate on brf?

I did make a mistake and put just a single silicone port in the center of the jar instead of 4 on the edges. Could that be why I can't see any growth?


It probably is the problem, actually. It's most likely growing from the inside out. Did you at least try to inoculate in different points, or just straight down?

Am I correct that nobody has gotten a liquid culture to work with this species? If it has been attempted would you list your recipe?


You know, I bet a malt extract-based LC would work well. It'd be just about the same nutrient base as MEA.

Oh yeah - I want prints when you have them. Of course.

#4 MLBjammer

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Posted 13 November 2009 - 05:55 AM

I have a six-pack of half-pints going. They have been slow to really take off, but my incubating temps have been inconsistent. I believe I am about two weeks in on these jars with maybe 30% colonization. I do have the standard 4-hole PF style lids on the jars.

KC, do you have air exchange ports on your lids?

#5 CaptainFuture

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Posted 13 November 2009 - 06:14 AM

In my experience Semperviva germinates around day5-6 if spores are fresh and then they grow like cubensis. Fast and dense but with no rhizomorphic grow.

Where did you get the spores?
Btw. if someone is interested, I've got prints left, pm me about details.

#6 kcmoxtractor

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Posted 13 November 2009 - 11:46 AM

Did you at least try to inoculate in different points, or just straight down?


I tried to inoculate by angling the syringe to the side, but it was difficult since my GB was so crowded at the time.

KC, do you have air exchange ports on your lids?


Yup, standard size poly stuffed holes.

Where did you get the spores?


I received the spores from a generous member on this site :bow:. They were from the fall of '08, so pretty darn fresh.


I think I'm going to try a few LC recipes, one malt based, [user]Irishlion[/user]'s karo/honey, and one of my own design.

#7 usagolden

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Posted 13 November 2009 - 01:19 PM

:headbang:
goodluck

#8 Lazlo

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Posted 13 November 2009 - 02:00 PM

Just hang in there. Inoculating like you did will take some extra time. Quite a bit, actually! You'll be fine, if the solution and substrate was competitor free.

#9 copelandiaKidd

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Posted 13 November 2009 - 02:05 PM

Best of luck!!!! cannot wati to see more of the Semperviva!

#10 kcmoxtractor

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Posted 13 November 2009 - 06:12 PM

LC is in the oven at 300F until it boils. Did 60mL of water, 3mL sugar, and going with 3cc of spore solution since it is kind of light.

#11 the_chosen_one

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Posted 13 November 2009 - 07:08 PM

LC is in the oven at 300F until it boils. Did 60mL of water, 3mL sugar, and going with 3cc of spore solution since it is kind of light.


maybe not as light as you think. the spores are about half the size of cubensis. ;)

i think i'll try an lc this weekend too. keep forgetting :rasta:

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#12 kcmoxtractor

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Posted 14 November 2009 - 12:01 AM

Noticed some germination in one of the jars :headbang: when the spot gets big enough I will try to get a picture.

LC is noc'd and waiting, and this time the spores were hydrated.

#13 MLBjammer

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Posted 14 November 2009 - 04:48 AM

Good to hear that you finally saw some germination.

#14 Thre365ive

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Posted 14 November 2009 - 11:47 AM

I knew you'd see some growth soon enough. Just make sure that next time you make 4 ports on your lids.

#15 kcmoxtractor

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Posted 15 November 2009 - 07:08 PM

Ok, I apologize, these are not the best pictures... but my LC is showing signs of growth! :headbang: This one is 3.6mL of sugars in 60mL of water, 1.8 of which are honey, 1.8 are karo, like Irishlion's tek. Sugar concentration is 6% in solution.

I'm going to give it another week, maybe two, then test it out on some PF jars.

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#16 MLBjammer

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Posted 15 November 2009 - 09:18 PM

Wow, that was fast. I think Irish's LC tek is the best one I have tried. TCO recommends millet for the Semper, but he also recommends millet for everything else, lol. I made PF style cakes with millet and vermic., and they are moving a little slow, faster in the last few days. Here is a picture, if you are interested. They are a few weeks into colonizing, maybe 14-16 days.

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#17 kcmoxtractor

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Posted 15 November 2009 - 10:35 PM

Those look nice, you can really see the how the mycelium is like cotton. Will you be spawning them, or fruiting them as cakes?

#18 libertaire

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Posted 15 November 2009 - 11:47 PM

Interesting.

#19 MLBjammer

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Posted 16 November 2009 - 04:13 AM

Those look nice, you can really see the how the mycelium is like cotton. Will you be spawning them, or fruiting them as cakes?


I have six of these, and I am not sure, to be honest. I am thinking of either casing them or spawning to straw. But I might try a couple of them invitro, just to see how they do.

Yeah, the mycelium is not rhizomorphic at all. It does seem to get thick, though.

Sorry to jack your thread, KC.

#20 libertaire

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Posted 16 November 2009 - 06:43 AM

I wonder if they would work as just cakes if it was the typical PF recipe of brf and verm.




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