
P. Semperviva
#1
Posted 13 November 2009 - 01:08 AM
They were inoculated on 11-5 and show no growth so far. How long do they normally take to germinate on brf?
I did make a mistake and put just a single silicone port in the center of the jar instead of 4 on the edges. Could that be why I can't see any growth?
I did the standard PF tek, and sterilized my jars for 50 mins at 15 psi. Used 10 of the 4 oz jelly jars, 1 half pint, and 2 pint jars.
Am I correct that nobody has gotten a liquid culture to work with this species? If it has been attempted would you list your recipe?
#2
Posted 13 November 2009 - 01:14 AM
about 5 - 15 days. my average is around 8-10 to see good germination in a four port jar.
:heart:
#3
Posted 13 November 2009 - 04:22 AM
This is my first attempt at an exotic species, so bear with me.
They were inoculated on 11-5 and show no growth so far. How long do they normally take to germinate on brf?
I did make a mistake and put just a single silicone port in the center of the jar instead of 4 on the edges. Could that be why I can't see any growth?
It probably is the problem, actually. It's most likely growing from the inside out. Did you at least try to inoculate in different points, or just straight down?
Am I correct that nobody has gotten a liquid culture to work with this species? If it has been attempted would you list your recipe?
You know, I bet a malt extract-based LC would work well. It'd be just about the same nutrient base as MEA.
Oh yeah - I want prints when you have them. Of course.
#4
Posted 13 November 2009 - 05:55 AM
KC, do you have air exchange ports on your lids?
#5
Posted 13 November 2009 - 06:14 AM
Where did you get the spores?
Btw. if someone is interested, I've got prints left, pm me about details.
#6
Posted 13 November 2009 - 11:46 AM
Did you at least try to inoculate in different points, or just straight down?
I tried to inoculate by angling the syringe to the side, but it was difficult since my GB was so crowded at the time.
KC, do you have air exchange ports on your lids?
Yup, standard size poly stuffed holes.
Where did you get the spores?
I received the spores from a generous member on this site :bow:. They were from the fall of '08, so pretty darn fresh.
I think I'm going to try a few LC recipes, one malt based, [user]Irishlion[/user]'s karo/honey, and one of my own design.
#7
Posted 13 November 2009 - 01:19 PM
goodluck
#8
Posted 13 November 2009 - 02:00 PM
#9
Posted 13 November 2009 - 02:05 PM
#10
Posted 13 November 2009 - 06:12 PM
#11
Posted 13 November 2009 - 07:08 PM
LC is in the oven at 300F until it boils. Did 60mL of water, 3mL sugar, and going with 3cc of spore solution since it is kind of light.
maybe not as light as you think. the spores are about half the size of cubensis. ;)
i think i'll try an lc this weekend too. keep forgetting :rasta:
:heart:
#12
Posted 14 November 2009 - 12:01 AM
LC is noc'd and waiting, and this time the spores were hydrated.
#13
Posted 14 November 2009 - 04:48 AM
#14
Posted 14 November 2009 - 11:47 AM
#15
Posted 15 November 2009 - 07:08 PM
I'm going to give it another week, maybe two, then test it out on some PF jars.
#16
Posted 15 November 2009 - 09:18 PM
#17
Posted 15 November 2009 - 10:35 PM
#18
Posted 15 November 2009 - 11:47 PM
#19
Posted 16 November 2009 - 04:13 AM
Those look nice, you can really see the how the mycelium is like cotton. Will you be spawning them, or fruiting them as cakes?
I have six of these, and I am not sure, to be honest. I am thinking of either casing them or spawning to straw. But I might try a couple of them invitro, just to see how they do.
Yeah, the mycelium is not rhizomorphic at all. It does seem to get thick, though.
Sorry to jack your thread, KC.
#20
Posted 16 November 2009 - 06:43 AM