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Psilocybe Ovoideocystidiata '09


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#21 Myc

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Posted 17 December 2009 - 06:51 PM

Hi Myc

That mycelium on the petri dish looks very healthy and aggressive.
What kind of medium is it ?
PDA, MEA or something else?


It's either one of the two........MEYA or PDYA ( I add nutritional yeast, thus the "Y" )
Bothe mediums support mycelial growth equally well. But you'll know soon enough. ;)

#22 Myc

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Posted 17 December 2009 - 07:01 PM

My plates aren't lookin' too bad, here's the "busiest" one. A few extra spores seemed to make their way onto the plate.

Ive never isolated before, do I need to let this grow out a bit before transferring?? .... can any of you see a nice section to grab ??

I kno the pic isnt that great, the random growth away from the center bit are actually looking the most aggressive, the central patch is thicker and whiter but isn't "reaching" out like the outer patches.

its nearly a week old now btw..

cheers:)


The way I do it is to grab the leading edge of mycelium - very faint - hold it up to the light and select opaque material - just a tiny little "snick" touch the scalpel to the desired transfer and dip the scalpel in the sterile agar - you wil be suprised if you are patient

#23 ethnobotanica

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Posted 17 December 2009 - 07:28 PM

Cool, thanks again Myc :)

#24 nightflyer

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Posted 18 December 2009 - 12:35 AM

It's either one of the two........MEYA or PDYA ( I add nutritional yeast, thus the "Y" )
Bothe mediums support mycelial growth equally well. But you'll know soon enough. ;)

Thanks! :)

#25 Myc

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Posted 05 February 2010 - 04:23 PM

Recent snowstorms provided an opportunity for a nice, cold dunk.

Projects were left outdoors for around 72 hours to endure a pretty heavy snowstorm - subsequent accumulation and melting of said snow - and drained on the 3rd day for return to the greenhouse.

I tried to part the grass a little to demonstrate the overlay
This is a textbook case - the mycelium is so dense at the casing layer surface, that it is near impervious to water.
Fortunately, when flooded, the blocks can absorb water like a sponge and hydrate from any direction.
A little scraping exposes a dense, ropy (and very tough), rhizomatic network of mycelial strands getting ready to produce fruit. :D

Over the next few weeks, I expect that we'll be seeing pins and hopefully, in a month or so.......fruitbodies. Fingers crossed.

One of the four tubs pictured is an Azurescens project which I'll cover in another thread.

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#26 hyphaenation

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Posted 05 February 2010 - 04:24 PM

Good work as always Myc.

Woodlovers Unite !

#27 Om shanti

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Posted 05 February 2010 - 04:30 PM

This will be good! Congratulations on the pins'n'mushrooms!! :dance: :eusa_danc

#28 paph

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Posted 06 February 2010 - 04:04 AM

Lookin Nice Myc.:thumbup::)

#29 Thre365ive

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Posted 06 February 2010 - 01:56 PM

Looking great, man! The print you sent me from these looked VERY nice under the microscope, too!

#30 Myc

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Posted 10 February 2010 - 11:18 AM

Looks like everthing is fine after the last storm.
I wonder if the mycelium will over-take the grass and become solid :eusa_thin

I suppose there's only one way to find out.
Photos demonstrate that the colony is well on its way to doing just that very thing!
I show one "bundle" of grass that must be peeled apart to become seperated.

The '08 project had fruitbodies with live grass going right through them.
After picking the grass had to be deliberately extracted from the stems.

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#31 copelandiaKidd

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Posted 10 February 2010 - 11:22 AM

lookin good brother. I cannot wait to see the results this time around...

#32 kcmoxtractor

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Posted 10 February 2010 - 12:39 PM

Wow... I love how aggressive they are! What are your temps like there?

#33 ethnobotanica

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Posted 16 February 2010 - 12:21 PM

Have you thought about "mowing" the grass to help at pickin time?:)

#34 Myc

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Posted 20 February 2010 - 05:42 PM

Have you thought about "mowing" the grass to help at pickin time?:)


Funny you should mention that .... I was going to try using a weed eater or powered string trimmer - scissors - shears - whatever works best.

Instead, I decided to try "birthing" the tubs - like giant PF BRF cakes

Temps have risen into the 50*F average - no drops to freezing at night-time temps stay 40*F or above
Imagine my surprise this morning when I discovered these little gems
Obviously, a casing layer isn't crucial to fruiting

I hope you will join me in my excitement - will post progress as it occurs

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#35 kcmoxtractor

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Posted 20 February 2010 - 05:46 PM

:eusa_clap: Wow man... just... wow....:eusa_clap

#36 hyphaenation

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Posted 20 February 2010 - 05:48 PM

Keep using the force on it Myc ...

:kewl:

#37 Om shanti

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Posted 20 February 2010 - 06:02 PM

Looks great, Myc. Congratulations! :bow:

You didn't notice the mushrooms and pins on the last pic from the 5th? Or is it only me
that is hallucinating? :-)

#38 Myc

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Posted 20 February 2010 - 06:13 PM


You didn't notice the mushrooms and pins on the last pic from the 5th? Or is it only me
that is hallucinating? :-)


Lol
Must be me who is hallucinating - I don't see them on the 2/5 photos
But I could be senile and blind too.

#39 Ben Dover

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Posted 20 February 2010 - 07:09 PM

:amazed:

Real nice Myc! Looking forward to what happens here :bow:

#40 the_chosen_one

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Posted 20 February 2010 - 07:39 PM

excellent work! beautiful. :love:




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