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Relay Grafting


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#1 Myc

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Posted 18 February 2010 - 10:35 PM

Purpose:
Maintain multiple substrains of similar genetics on a single rootball
Difficulty rating:
Falling off a log easy
Two live plants were scarred and bound together. Care was taken to observe aseptic technique and wounded areas were treated with rooting compound to aid in recovery.
Wounded areas were then wound with breatheable laboratory film to support the graft and exclude contaminants.
Prep the receiving area by extracting a " V " shaped groove several millimeters long
Prep the donor by shaping a clone-worthy branch into an inverted " V " so the donor and host fit together neatly
The wounded mateial is allowed 14 days to "mate" or "knit"
Then the donor plant is cut away
The scion is now part of the original rootball
Pictured here are
Jack Herrer #2 - Main rootstock
Afghan #1 - donor plant/scion
Using this method insures both plants the greates chances of a healthy recovery. The variety of scions supported by the parent rootball is limited only by the imagination.
Previous experiments with "cut" scions (as in standard cloning) failed repeatedly. Maintaining elevated humidity proved difficult and the scions rarely lasted more than a few hours.

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#2 Dipole

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Posted 18 February 2010 - 10:46 PM

Amazing.

#3 shroom57

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Posted 18 February 2010 - 11:00 PM

That's bordering on awesome, and I don't usually say that...

#4 dice

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Posted 18 February 2010 - 11:09 PM

That's bordering on awesome, and I don't usually say that...


Na man that IS freaking awesome.
Id say almost amazing
Myc you got one hell of a green thumb there man:bow:

#5 ou3308

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Posted 18 February 2010 - 11:29 PM

Iv'e grown quite a bit in many different ways but are you just sick of normality? That is coooool!!!!

#6 Myc

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Posted 18 February 2010 - 11:30 PM

Seed usually come multiples to a package
different phenotypes peform differently
some genetic stock gets "passed by" because its sister plant is more robust or a better performer

This science allows the patient hobbyist to preserve all of the feminine products of a single seed purchase onto a single - robust - proven rootstock - and experiment with them further
high yield - low THC
high yield - high THC
low yield - ............you get the idea - over time genetic continuaton and chromosome work?? may become obvious

Perhaps if one were to experiment with ruderalis? hops?
The point demonstrated here is that Afghan #1's genetic characteristics will continue to replicate using Herrer # 2's water supply
What if an intensely robust rootstock were substituted?

#7 shroom57

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Posted 20 February 2010 - 08:58 PM

Now that I've had a moment to think about it and digest it...

ok, it's awesome...lol

the part I need some help with is this...

The wounded mateial is allowed 14 days to "mate" or "knit"
Then the donor plant is cut away
The scion is now part of the original rootball


the donor plant is cut away?

#8 greenie

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Posted 20 February 2010 - 11:39 PM

i was going to try GDP on a trainwreck or green cfack rootstock... do u think the larger root genetics of those plants would produce a monster GDP plant, or just a drought resistant one?

#9 Soliver

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Posted 21 February 2010 - 12:22 AM

I've always heard that weed can be spliced to a hop vine... never tried it. I have weed,
I have hops (well, when they pop up again in the summer), but have never thought to
try it..

:)

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#10 Myc

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Posted 21 February 2010 - 09:57 AM

The science is actually pretty neat

Take two living plants - two seperate rootballs
Surgically scar them with my "tongue and groove" method
Place the concave and convex " V " -shaped stock together
Paint with rooting hormone
Wrap with Parafilm

After 14 days, one of the plants is cut away - you choose which rootball becomes the parent at this point

It is this natural principal which allows organ transplants to work for human beings.

Once the foreign cellular material (scion) bonds to the parent,
The parent plant will "feed" the scion and allow it to continue growing

However - the two plants DO NOT exchange genetic characteristics
The only thing they share in common is a food supply

For example,
A hops plant grafted to a cannabis rootball will not become active and produce THC - and vice versa
A cannabis plant grafted to a hops rootball will continue to grow - business as usual - and remain active

I saw this idea on a youtube video
But it was just a tour of some guy's shoppe in Amsterdam
They were applying this science in a number of ways - but failed to explain "how to". I just reverse-engineered their idea and practiced until I got a graft to take using various methods

His experiments were:
Attempt to increase yeild - graft quality cannabis onto more robust rootstock -- In the words of the shoppe owner - this didn't work like we had hoped
But I don't know how far they pursued the tek
Their findings showed that a "poly" mother plant was the best application of this science

This experiment is currently nearing the 14 day mark - so the "cutting away" step can be better demonstrated
Stay tuned and I will follow this out to new growth and subsequent cloning.

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#11 greenie

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Posted 21 February 2010 - 10:33 AM

excellent myc. So it seems this would be an excellent way for someone dealing with 'plant counts' to maximize their variety of medicine. rock on!

#12 tenjin

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Posted 21 February 2010 - 10:44 AM

Great work Myc, very interesting :bow:

#13 demetrius shardson

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Posted 21 February 2010 - 11:00 AM

the best of waking up...... i love topia awesome thread myc

#14 prism

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Posted 21 February 2010 - 11:58 AM

Can you draw the scoin and rootstock pre graft? Id like to see the "V-grooves" before they are grafted.

Awesome Job!!:bow::bow::bow:

#15 rockawayrooms

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Posted 21 February 2010 - 12:02 PM

AWESOME work Myc!!!:bow::bow::bow:

#16 Xchatta

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Posted 22 February 2010 - 08:42 PM

so could u just have one large rootball and continue cutting and pasting in branches as u need them, or does ur graft and ur plant need to be in veg and then only after the 14 days put into flower? or is it so that u can have many strains and only one mother?

#17 Foster

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Posted 22 February 2010 - 08:51 PM

Awesome work Myc! :weedpoke::bow:, Keep it green brutha! Great Stuff!

#18 Myc

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Posted 22 February 2010 - 10:33 PM

so could u just have one large rootball and continue cutting and pasting in branches as u need them, or does ur graft and ur plant need to be in veg and then only after the 14 days put into flower? or is it so that u can have many strains and only one mother?


Many strains - one mother
Trying to flower such a plant would be a real chore

I will do a step-by-step of the grooving process
This mom is going to have quite a family before she's done.

BTW,
Thanks to all of you for the looks, questions, and comments.
I appreciate them - and consider myself a student here -
Thanks for teaching me.

#19 prism

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Posted 23 February 2010 - 12:41 PM

I look forward to your update!:rasta:

#20 Xchatta

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Posted 24 February 2010 - 12:29 AM

ah its brilliant:love::greenboun :eusa_clap




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