First, I'll include what I did. I do not have a flow hood, but extreme caution was used. I did this in a closet with a HEPA filter running for the last several months. I washed my hands thoroughly, rubbed my hands and the spoon with alcohol, then flamed the spoon. I was wearing clean clothes, and had just gotten out of the shower.
I had 3 "mother jars".
One with WBS that had stalled, completely, only a small splotch of myc. Noc'd this up quite some time ago, it was my very first attempt at WBS. It was from a pint jar. WAY too dry in there. (MALABAR strain from Sporeworks). The jar was about 10% colonized.
Second jar was another WBS jar that had completely stalled. (ECUADOR from Sporeworks) Now - I know they were viable syringes, because I'd fruited from these using BRF cakes, so it's not Sporeworks fault - it was my WBS tek. This one had maybe 3% colonization in a pint jar. Literally just one splotch of myc to take from. I was not expecting any success here.
Third jar was a fully colonized quart jar of popcorn (Burma strain from TGS). I spawned this to a a pint jar, and a pair quart jars. I used the rest of this quart to spawn a rez tray. (See my grow logs in my sig). The pint jar I'm just making to do more g2g transfers if all turns out well.
So here are the pictures.
We'll start with the Ecuador. The jar I thought LEAST likely to succeed.
http://mycotopia.net...=1&d=1268912332
This was done less than 48 hours ago, and it's already come back to life. :pirate: It's taking over the corn in some places, and looks to be making quite a recovery in it's new - more suitable environment.
Next, this is the Malabar jar. Again, this is less than 48 hours after the transfer from WBS to popcorn.
http://mycotopia.net...=1&d=1268912332
I'm extremely impressed with this strain. Malabar has crazy colonization times, and has shown itself to be very resilient in my experience.
Here is the first quart of the burma g2g. This was from healty mycelium, and had a good spoonful put into it. This was less than 72 hours ago.
http://mycotopia.net...=1&d=1268912332
Here is the second quart of the burma g2g.
http://mycotopia.net...=1&d=1268912332
And finally, here is the pint jar. This is going to be several jars "mother", as I plan on using this only for g2g transfers.
http://mycotopia.net...=1&d=1268912332
So far the conclusion I've drawn is this will be the way I proceed from now on. I'm loving g2g so far. :) I plan on just ordering a lot of different strains, and keeping a "mother jar" for all of my quart jars. It's pretty wild how fast it's colonizing, especially considering a lot of it came form myc that I had written off for dead!
Edited by IncredibleEdible, 18 March 2010 - 06:55 AM.