Here's my GB....anticipating all that follows..[ glovebox merged ]
Posted 12 October 2005 - 11:25 PM
Posted 13 October 2005 - 06:33 AM
The gloves seem to cling well without any reinforcement..
Make sure u can slip your hand in and out without too much resistance...
The best glue i have found for bonding the glove assembly to the box is "Goop" -make sure to roughen both surfaces with sandpaper to increase the strength of the joint...As for sealing the lid - it's hard to know without seeing the box but a combination gasket +tape or a weight ..has to be easy to get in and out of...gluk...
Posted 13 October 2005 - 01:01 PM
My box is a rubermaide and the top is indeed not very airtight. I dont mess with no saran wrap except the window cut in the lid in the middle is saran wrap duct taped. I have heard of people putting down some weather stripping to help the seal. I dont worry about it myself. Maybe I should.
Posted 14 October 2005 - 11:59 AM
Using plastic "Big Gulp" cups cut in half for glovebox holes makes it very easy to attach rubber gloves to with rubber bands to hold 'em in place. Would sealing these cups with caulk silicone hold them in place and resistant to contams?
i did just exactly that with one of my earlier 'boxes,
worked well enough.
Posted 14 October 2005 - 01:59 PM
Posted 17 October 2005 - 11:44 AM
It’s my rabbit’s first time using a glovebox and after shooting 6 BRF jars yesterday, he finds he has some questions about how to best sanitize his glovebox and what workflow to use to successfully inoculate jars.
Should he be spraying the inside of the glovebox with Lysol or bleach? Yesterday, he used a store-bought Clorox-Clean-Up spray bottle, but he’s heard people in the archives talk about diluted bleach, and wonders what on earth that is.
Should he be spraying his glovebox before he puts jars and syringes inside or after? And should he be worried the fumes will do unmentionable things to his spores?
Should he be wiping the tops of his jar lids with bleach after removing the foil and before shooting them up? Yesterday, my rabbit used a lighter to sterilize the syringe needle between each jar, should he continue doing that next time?
(Also, my rabbit had a hard time controlling the flow of liquid from his syringes and is pretty sure a couple of jars got over 2ml of sweet spore water, not what he had in mind.)
My rabbit would greatly appreciate a step by step workflow from any of you who are using or have used a similar glovebox set-up.
Just fyi, the first time my rabbit tried his hand at microscopy, he simply used a clean bathroom for spore injection so he’s finding the glovebox extremely cumbersome, but since he spent a few hours building it, he is committed to learning how to use it well :-]
Posted 17 October 2005 - 12:00 PM
Posted 17 October 2005 - 01:18 PM
I would reccommend waiting at least 15 minutes before starting.
Posted 17 October 2005 - 02:29 PM
They are a pain to work in but I found after the first few tries I was used to it enough and happy that I could do sterile work without worries of contams or sterilizing an open area. The most cumbersome is the gloves and where your arms enter. If you can find a way to make that comfy, your rabbit will be happy :)
Posted 17 October 2005 - 09:47 PM
pf style jars can safely be inoculated in open air,
no glovebox needed.
Posted 18 October 2005 - 08:55 AM
My rabbit was considering wiping down jar lids with alcohol before injection, but wasn't at all sure that would do the job, given how quickly the 91% iso evaporates, but he will take freakachino's advice and try it next time.
Thanks for all the other advice, too, though my rabbit is always happy to get even more pointers so he can become a Zen Master of Cleanliness!
Posted 18 October 2005 - 09:09 AM
Posted 18 October 2005 - 09:12 AM
for not-so-obvious reasons
the 70% seems more effective than higher concentrations.
less explosive/flammable, too, although still dangerous.
Posted 18 October 2005 - 09:23 AM
Or should I not even bother putting foil back on? My incubator is running at ~82F so I will get some condensation and was thinking the foil will prevent the condensation from leaking into the jars.
Posted 18 October 2005 - 09:25 AM
i'd lower the heat to 80,
glass jars trap heat so it's hotter inside.
Posted 18 October 2005 - 09:43 AM
One (hopefully) last question - what's the best way of sterilizing the syringe needle?
laborer (up above) suggested dipping it into alc, but I just got some really long (3') needles for my Karo jars and there's no way I can figure out a way to dip them, since they're so damn long. Should I be sterilizing the needle between jars to prevent cross-contamination?
Posted 26 February 2006 - 06:16 PM
Posted 26 February 2006 - 08:46 PM
Question! How often to I sterilize the needle per syringe? If I am changing syringes...do I only sterilize per syringe?
If I get a great batch, ie, alot of viable jars, what do you think of casing a few, and what TEK do you recommend for casing?:flamer: