55 replies to this topic

[Hippie3]

the pix

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[pistole_peat2000]

HEy there hip just wondering what isall this then

[elixir]

HEy there hip just wondering what isall this then

A simple spill-proof method for filtered air injection to liquid cultures.

[Hippie3]

and more.
this is an integrated system,
actually.
the polyfilled syringe acts as an air filter,
the silicon ports are self-sealing after injection.
can be used to get liquids out of sealed jars/bags,
inject liquids/spores/nutes into sealed jars/bags,
let filtered air in/out as needed, where needed.

[bohner]

and here is a link to my post on how to automate your airports [to bubble liquid culture jars ]

http://mycotopia.net...read.php?t=5177

But i'm not a fan of that rtv sealant that hippie uses i would just go to a hardware store and buy a silicone sealant that says for high temperatures

my next experiment will be to try this airport on some BRF cakes to see about contams. may even be a glovebox-less alternative but i'm getting ahead of myself must test first

[Hippie3]

rtv silicon adhesive, safe at high temps, got it at auto parts store,
works great for me so far,
takes repeated injections and seals every time too.

stress tests:
I injected a 6mm thick silicone blob 200 times in a row with a syringe. It still sealed like a champ, though it was degraded somewhat. I PCed an airport lid at 15psi for 1.5hrs six times with no notable deletrious effects (other than no rust on the holes, 'cause they were sealed in silicone!).

[shobimono]

Cross reference to airport in action
http://mycotopia.net...read.php?t=5001

[Hippie3]

more pix

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[Hippie3]

copied to the vaults

[Hippie3]

also see
http://mycotopia.net...read.php?t=5212

[RedOctober]

So you don't autoclave the polyfil? I was wondering about this because it looks like in the pictures you take it straight out of the bag and pack it into the syringe with a pen. How come it doesn't need to be steralized?

[Hippie3]

i sterilized the entire airport after making it
by wrapping it in tinfoil and pc'ing.

[BuckarooBanzai]

Those silicone injection sites are simply an amazing idea. I shopped around forever looking for something I can now make a thousand of for $10. Hippie3, you should be working with NASA. We could get to Mars for $300 worth of parts from eBay...

Vocabulary/syntax question: does "airport" refer to just the silicone sealed hole, or the hole and poly syringe together?

I use GE Silicone II clear sealant/caulk (max temp 400F). $6 from Wal Mart for a tube of goo that will make about 60 lids (I like a BIG blob of silicone). I put a blob of white silicone II on the underside of the lid. It makes the injection hole easier to see in my poorly lit/ghetto glove box.

I have a little circle of tyvek silicone glued across the top of my polyfill/input syringe. It keeps moisture out of the poly in the PC.

Stupid stress tests:
I injected a 6mm thick silicone blob 200 times in a row with a syringe. It still sealed like a champ, though it was degraded somewhat. I PCed an airport lid at 15psi for 1.5hrs six times with no notable deletrious effects (other than no rust on the holes, 'cause they were sealed in silicone!).

Is there a Nobel-type prize for assininely good ideas in this hobby? There should be...the grass roots innovations in technique I have read about here would make any research scientist stand up and take note! This is what happens when you give people a forum...aren't we glad Al Gore invented the Internet?????

[Hippie3]

Hippie3, you should be working with NASA. We could get to Mars for $300 worth of parts from eBay...

:lol:
stop, you're making me blush...:eusa_liar

[Hippie3]

Vocabulary/syntax question: does "airport" refer to just the silicone sealed hole, or the hole and poly syringe together?

i see it as a 'system' as both are needed
so either part could properly be called an airport
alone or in combination

afterthought:
perhaps the syringe part should be the 'airport'
and the silicon blob called a 'port'

[Hippie3]

I have a little circle of tyvek silicone glued across the top of my polyfill/input syringe. It keeps moisture out of the poly in the PC.

nice touch
but proly un-needed
i just wrapped in tinfoil for the once thru the pc

[BuckarooBanzai]

I'm a big fan of overkilling a process (vis a vis the tyvek circle and the huge blobs of silicone). It's just my way...

"The palace of widom is gained by the path of excess."
-William Blake

"Anything in life worth doing is worth overdoing."
-Me

[Hippie3]

I injected a 6mm thick silicone blob 200 times in a row with a syringe. It still sealed like a champ, though it was degraded somewhat. I PCed an airport lid at 15psi for 1.5hrs six times with no notable deletrious effects (other than no rust on the holes, 'cause they were sealed in silicone!).

pretty tough stuff, eh ?
;)

[Hippie3]

:lol:
what can i say...