Paradox
©
Fisana

Jump to content


Photo
* * * * * 2 votes

Psilocybe aztecorum - Pictures and Microscopy


  • Please log in to reply
30 replies to this topic

#1 Alan Rockefeller

Alan Rockefeller

    Mycologist

  • OG VIP
  • 2,061 posts

Awards Bar:

Posted 22 September 2010 - 07:56 PM

Location: Nevado de Toluca, Mexico State, Mexico. 50 miles west of Mexico City.
Elevation: 11,420 feet
Substrate: Stumps of the bushes pictured in this observation.
Spores almond shaped, 8.0 . 10.0 × 4.7 . 6.0 micrometers. Q = 1.50 to 1.92, Median Q = 1.63
Spore measurements:

8.0 × 4.7
8.1 × 5.0
8.5 × 4.7
8.5 × 5.6
8.5 × 5.6
8.6 × 5.4
8.7 × 5.4
8.8 × 5.1
8.9 × 5.5
9.0 × 5.1
9.0 × 6.0
9.1 × 5.7
9.2 × 5.1
9.2 × 5.8
9.3 × 5.7
9.3 × 6.0
9.4 × 5.4
9.5 × 5.8
9.5 × 6.2
9.7 × 5.8
9.8 × 5.8
9.9 × 5.4
10.0 × 6.0

24.jpg

Hippie is a good dog. Does not bark or bite. It was not easy to get him to sit still for this picture.
25.jpg

Cheilocystidia
29.jpg

Pleurocystidia
38.jpg

Pileipellis
40.jpg

Pileus trama
41.jpg

Cheilocystidia crush mount, 1 micron divisions
49.jpg

Stained with congo red
50.jpg

Pleurocystidia crush mount, 1 micron divisions, stained with congo red
56.jpg

Spores, 1 micron divisions
61.jpg

With a cheilocystidia
63.jpg

Higher resolution images: http://mushroomobserver.org/53482

Attached Thumbnails

  • 60.jpg
  • 62.jpg
  • 58.jpg
  • 59.jpg
  • 57.jpg
  • 55.jpg
  • 54.jpg
  • 52.jpg
  • 53.jpg
  • 51.jpg
  • 48.jpg
  • 45.jpg
  • 46.jpg
  • 47.jpg
  • 44.jpg
  • 43.jpg
  • 42.jpg
  • 39.jpg
  • 35.jpg
  • 36.jpg
  • 37.jpg
  • 34.jpg
  • 33.jpg
  • 32.jpg
  • 31.jpg
  • 30.jpg
  • 26.jpg
  • 28.jpg
  • 27.jpg
  • 23.jpg
  • 22.jpg
  • 20.jpg
  • 21.jpg
  • 19.jpg
  • 18.jpg
  • 14.jpg
  • 15.jpg
  • 17.jpg
  • 16.jpg
  • 11.jpg
  • 12.jpg
  • 13.jpg
  • 09.jpg
  • 10.jpg
  • 08.jpg
  • 05.jpg
  • 07.jpg
  • 06.jpg
  • 04.jpg
  • 02.jpg
  • 03.jpg
  • 01.jpg

Edited by mydarling, 24 September 2010 - 05:54 PM.
uploaded 63 photos to topia & converted to thumbnails

  • warriorsoul, Arathu, lipase and 4 others like this

#2 MrChen

MrChen

    Rocky Mountain High

  • Expired Member
  • 2,098 posts

Posted 22 September 2010 - 08:22 PM

Awesome finds Alan! :headbang: Thank you for sharing!

:kewl:

#3 kcmoxtractor

kcmoxtractor

    Halloween Terror

  • Honorary Former Staff
  • 6,904 posts

Donator


Awards Bar:

Posted 22 September 2010 - 08:32 PM

Archive material :bow:
Thanks alan!

Are they growing from the thatch layer
or from buried wood?

#4 Alan Rockefeller

Alan Rockefeller

    Mycologist

  • OG VIP
  • 2,061 posts

Awards Bar:

Posted 22 September 2010 - 08:49 PM

They are growing from buried wood.

I am going to visit the area again in a couple days, I will try to get some better pictures. I am in Mexico City right now and its raining like crazy.

#5 mydarling

mydarling

    (actual mycologist)

  • Expired Member
  • 4,025 posts

Posted 22 September 2010 - 09:06 PM

thanks alan, great photos! i will upload those to topia for you :)
  • Freaky likes this

#6 Dr.Hallucination

Dr.Hallucination

    Chasing the Rabbit.

  • Honorary Former Staff
  • 6,480 posts

Awards Bar:

Posted 22 September 2010 - 09:08 PM

:bow::bow::bow:

#7 loudenbomber

loudenbomber

    Anti X-tian

  • Expired Member
  • 264 posts

Posted 22 September 2010 - 09:54 PM

wow . I love seeing real finds of species not often talked about. This is truly a great find.

Best of luck on finding something new and bringing it back :bow:

#8 -=Zeus=-

-=Zeus=-

    -= In The Flow =-

  • OG VIP
  • 2,019 posts

Donator


Awards Bar:

Posted 22 September 2010 - 09:55 PM

Breathtaking photos! I love seeing the unusual exotics in the wild :)

#9 Raoul Duke

Raoul Duke

    Mycophiliac

  • Expired Member
  • 782 posts

Posted 22 September 2010 - 10:15 PM

Cool stuff as usual Alan. Your travels take you to some exotic locals, good luck on the hunt.

#10 warriorsoul

warriorsoul

    Retired Moderator

  • Honorary Former Staff
  • 4,802 posts

Donator


Awards Bar:

Posted 22 September 2010 - 11:22 PM

Nice pics, your spores are smaller than what is recorded for P. aztecorum?

Your specimen 8.0 - 10.0 × 4.7 - 6.0
P. aztecorum (10.4)12 - 14.0(17) x (6)6.6 - 7.7(8.8)
var. bonetti (9)10 - 13.0(14) x 6.0 - 7.5(8.0)

#11 Alan Rockefeller

Alan Rockefeller

    Mycologist

  • OG VIP
  • 2,061 posts

Awards Bar:

Posted 23 September 2010 - 12:38 AM

wow . I love seeing real finds of species not often talked about. This is truly a great find.



According to Psilocybin Mushrooms of the World this species is only found on this one mountain, but I think some of the Guzman papers reference it from Veracruz as well. In any case its very rare and only grows at very high elevation. Probably needs it cold to fruit. Cactu has a theory that this species was more widespread in the ice age and now only survives in the high elevation areas.

I think there are many fungi in this place that are unknown to science, I am going back there in a few days to make some more collections.


Breathtaking photos! I love seeing the unusual exotics in the wild :)


This is just the beginning, I have been in Mexico for over a month and have thousands of photos to upload still. I put them on mushroomobserver.org first and make threads for the message boards when I complete the microscopy / get all the pics from one place uploaded.

There are still so many places to go - I am in Mexico City now, I am going back through Michoacan to Jalisco, then to Colima, then back to Mexico State, then Veracruz, then back to Jalisco to meet with the president of the city where Cactu lives to get them to start a fungus fair there, then to Oaxaca and who knows where else. I intended to only go to Mexico for 3 weeks but the Mexicans are fqn cool and there are so many places to go that I keep moving my return flight back. I am going to stay here until the rainy season starts back up in California.


Nice pics, your spores are smaller than what is recorded for P. aztecorum?

Your specimen 8.0 - 10.0 × 4.7 - 6.0
P. aztecorum (10.4)12 - 14.0(17) x (6)6.6 - 7.7(8.8)
var. bonetti (9)10 - 13.0(14) x 6.0 - 7.5(8.0)


Interesting, I have been so busy working on these photos that I have not compared them with Guzman´s description.

I will have to get the original paper by Heim and see what his description says. Since this species was described in 1957 I can probably get the type collection and sequence it and see how this material matches up.

I do not think my numbers are off - I have measured the spores from some other species from the same area and they turned out larger than expected, and most of the spores from the species I have measured are the size that I thought they would be. I did the measurements using the gimp to count the number of pixels in each micrometer on the reticle (in this case they pictures were 6.1 pixels per micron), then measuring the number of pixels long and wide each spore is and dividing by 6.1. It takes a bit of time but eliminates the guesswork from measuring spores. It will be interesting to see how the cystidia compares.

Here is a quick video of the mushrooms:

[Direct Link]



Its not really easy to make a video through the microscope but I did it anyway, this video shows some of the unique pleurocystidia:

[Direct Link]


Edited by the_chosen_one, 13 May 2014 - 05:35 PM.
repaired video links


#12 warriorsoul

warriorsoul

    Retired Moderator

  • Honorary Former Staff
  • 4,802 posts

Donator


Awards Bar:

Posted 23 September 2010 - 09:33 AM

I assume your reticle is calibrated?

Heim originally placed P. aztecorum(based on collections from Wasson) as a variation of P. mexicana (P. mexicana var. longispora).

I'd like to see this paper.
http://www.jstor.org/pss/3759037

#13 demetrius shardson

demetrius shardson

    be informed, then choose.

  • Expired Member
  • 654 posts

Posted 23 September 2010 - 09:44 AM

awesome finds and photos as usual alan, thanks for sharing.

#14 copelandiaKidd

copelandiaKidd

    I am

  • Free Member
  • 1,563 posts

Posted 23 September 2010 - 10:17 AM

a few of those pics lookes to me like cyanofriscosas.... and the pics iv sen of aztecorum, were more conical. maybe im confusing species......?! hmmmm. idk

#15 TastyBeverage

TastyBeverage

    Goodbye Ofelia

  • Expired Member
  • 5,229 posts

Posted 23 September 2010 - 10:33 AM

thanks alan, great photos! i will upload those to topia for you :)


THANK YOU, MYDARLING!!!!!!

:loveeyes::loveeyes::loveeyes::loveeyes::loveeyes:

You are a trooper! Excellent work. Thank you, thank you.

#16 Alan Rockefeller

Alan Rockefeller

    Mycologist

  • OG VIP
  • 2,061 posts

Awards Bar:

Posted 23 September 2010 - 11:54 AM

I assume your reticle is calibrated?


No. I will see if I can borrow a stage micrometer from the University of Guadalajara.

I'd like to see this paper.
http://www.jstor.org/pss/3759037


I sent out an email and will hopefully receive it soon.

a few of those pics lookes to me like cyanofriscosas.... and the pics iv sen of aztecorum, were more conical. maybe im confusing species......?! hmmmm. idk


The picture in Psilocybin Mushrooms of the World does look a bit different. Thanks for pointing that out.

Some habitat shots:
img_1858.jpg

A video of the mushrooms:

[Direct Link]



Its not easy to take video through the microscope but here is my first attempt at capturing the unique pleurocystidia:

[Direct Link]

Attached Thumbnails

  • img_1857.jpg
  • img_1856.jpg

Edited by the_chosen_one, 13 May 2014 - 05:37 PM.
repaired video links


#17 dial8

dial8

    3 Fungi Mod

  • Honorary Former Staff
  • 5,767 posts

Donator


Awards Bar:

Posted 23 September 2010 - 01:44 PM

Very nice as always, Alan. :bow:

I was in Merida Mexico a short while ago but did not get a chance to look. Im going back for a week in october and should be able to look then. Im also going to be in Zihuatanejo next June. That trip is going to be strictly pleaseure so I should definitely get some hunting done. Any recomendations??

#18 wearegomba

wearegomba

    Mycophiliac

  • Expired Member
  • 88 posts

Posted 23 September 2010 - 01:47 PM

Beautiful photos
Solid mycological work on a rare interesting fungi
Way cool post, thanks for sharing

#19 TastyBeverage

TastyBeverage

    Goodbye Ofelia

  • Expired Member
  • 5,229 posts

Posted 23 September 2010 - 01:48 PM

Thanks for uploading the follow up pictures, MrChen!!!!! You rock!!!!

:bow::bow::bow::bow::bow::bow:

#20 Alan Rockefeller

Alan Rockefeller

    Mycologist

  • OG VIP
  • 2,061 posts

Awards Bar:

Posted 23 September 2010 - 10:21 PM

Very nice as always, Alan. :bow:

I was in Merida Mexico a short while ago but did not get a chance to look. Im going back for a week in october and should be able to look then. Im also going to be in Zihuatanejo next June. That trip is going to be strictly pleaseure so I should definitely get some hunting done. Any recomendations??


Merida is very beautiful.

October is late in the season but maybe not too late.

June is early in the season but maybe not too early.

Thanks to the nice moderators who uploaded my pics for me. I think the offsite URLs I use are permanent but you never really know.....

When you go hunting find steep ravines and gullies, you will never find any actives walking around the woods in flat places. You will find actives in the fields but they will be really boring actives like P. cubensis, Panaeolus cyanescens and Psilocybe mexicana. The really cool stuff is in the most fucked up places.

Dozens of times Cactu and I have been searching for mushrooms and I want to tell him "hey man lets go back to the car, there is nothing here, we have been looking for hours and I am cold and wet and its starting to rain really hard and this is a waste of time." But I hold my tongue and say nothing. Some time later I hear an excited shout from deep in some steep ravine and a new patch is discovered.




Like Mycotopia? Become a member today!