Honey Liquid culture [merged]
Posted 26 October 2005 - 09:12 AM
how do you prep the jars ?
how do you inoculate and with what ?
Posted 03 December 2005 - 10:23 PM
Posted 03 December 2005 - 10:47 PM
Posted 04 December 2005 - 12:45 AM
but read that all HONEY is not necessarily the same.....
What kind are you using :rasta:
Posted 22 March 2006 - 12:05 PM
How do you rate my chances folks??
I will soon be getting back into agar and I feel as though I need to make my own glove box!!! need to get motivated! any easy and cheap options?
thanks in advance
Posted 22 March 2006 - 10:53 PM
My pet lemur digs hers. She made a fancy one with fan and hepa filter but finds it was a waste of time and cash.... she now using it with the fan off. Just still air works and don’t have any contams being blown around your work space. If she was to remake she would ditch all the fans etc and spend the saved cash on decent gloves and silicone sealant for around the glove flanges and window. She also digs having the racks as the keep your work of the deck and gives you a dumping space....
Any ideas for good gloves? My lemur like being tactile with the hands and able to move freely... tyvke sleves and latex glove???
Posted 23 March 2006 - 03:25 AM
Posted 23 March 2006 - 08:01 AM
Posted 23 March 2006 - 10:38 AM
Posted 23 March 2006 - 10:33 PM
Thanks for the advice
Posted 15 April 2006 - 03:51 PM
this season I've made a few(5) honey water jars (1-2 tbs honey : 1 pint h2o) using multispore innocs of PE and EQ. So far not much has come of the honey water jars, the only growth I've gotten in the jars has been very scattered and loose, almost nothing. However grain jars I made with the same spores have thrived in the same conditions.
It got me to wondering, should I shake up the honey jars to get more O2 into the water? Ive done honey jars in the past with tissue and they've worked great but perhaps spores just dont have the strength to start up a colony without more suitable conditions.
Just a thought.
Does anyone think this is likey?
Posted 15 April 2006 - 04:03 PM
tyvek or polyfil getting wet is a no-no.
i use silicon ports so i shake mine daily for aeration.
Posted 15 April 2006 - 04:11 PM
I was thinking of covering the jars with plastic rap (after nuking ofcourse) and then putting a lid with 4 polyfilled holes on top Secure the lid, give a nice shake, innoc through the polyfil and through the plastic rap and there we go.
Seem do able?
I dono if the plastic rap might leak or anything. :shy:
Any take on it?
Posted 15 April 2006 - 04:13 PM
In that case I might try to get my hands on some of that silicon paste or whatever.
Posted 15 April 2006 - 04:43 PM
you'll have wet polyfil
even worse it's got honey in it.
so, no. bad idea.
but growth is faster with some air
so i use silicon adhesive with is water-proof,
Posted 16 April 2006 - 07:18 PM
Shake it up VIOLENTLY right after coming from the PC. The PC process will drive virtually all the oxygen out of the liquid.
Once it starts colonizing, the more often you shake it, the faster it will go. Bluehelix has demonstrated liquid cultures finishing in 3 days with constant mechanical stirring. FOAF prefers to swirl, instead of shaking, after growth is visible. Others have differing opinions.
Posted 14 June 2006 - 04:20 PM
If pure honey wont work, he would like to know where to find the other nutes mentioned as he has never seen them before. and what would be easiest and most foolproof to try for his first time
" Karo Light with Vanilla
Light malt extract
Posted 14 June 2006 - 04:29 PM
You don't want light, though. Just regular Karo.