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cloning oyster tissue to grain


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#121 ethnobotanist420

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Posted 24 December 2010 - 01:03 AM

oh yeah, timelapse when i get back too :headbang:


Lookin forward to that :loveeyes: have a good Christmas!

#122 mydarling

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Posted 27 December 2010 - 11:55 PM

:gah: :gah: :gah: :gah: :gah: :gah:

compiling this time lapse is really making my head hurt.
i've been working on it for a couple days, but it's really hard !!!!!!!
trying to teach myself how to use GIMP .....
i did an animated GIF a few years ago but i was using windows at the time so i had different software.

:gah: :gah: :gah: :gah: :gah: :gah:

anyway, so i do have pics of the fruits before & after harvest from a couple days ago. i could post those up in the meantime. :)

i left the 5 biggest mushrooms to their own devices over the weekend, and sad to report they just dried up :( shoulda figured that would happen, with no misting ....

i'll go work on the harvest pics now, i need a break from GIMP! :gah:

#123 mydarling

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Posted 28 December 2010 - 12:16 AM

alright here are the last pics.

http://mycotopia.net...=1&d=1293513034

http://mycotopia.net...=1&d=1293513034

http://mycotopia.net...=1&d=1293513034

http://mycotopia.net...=1&d=1293513034

http://mycotopia.net...=1&d=1293513034

http://mycotopia.net...=1&d=1293513034

http://mycotopia.net...=1&d=1293513034

http://mycotopia.net...=1&d=1293513034

this one was trying to escape out the top!

http://mycotopia.net...=1&d=1293513034

the harvest

http://mycotopia.net...=1&d=1293513084

http://mycotopia.net...=1&d=1293513084

these are the ones that grew from the top of the jar, getting more crooked and malformed as they sought the opening

http://mycotopia.net...=1&d=1293513084

i harvested all except these couple big ones, hoping they'd get bigger while i was gone for xmas.

http://mycotopia.net...=1&d=1293513034

but they just shriveled up and dried out after that, didn't get any bigger. no misting = no growing. oh well!

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#124 justweed

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Posted 28 December 2010 - 11:15 AM

Very awesome MD, those fruits are excellent. You should have snapped some pics of the dried up fruits on the sub still :D

#125 mydarling

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Posted 09 January 2011 - 03:12 AM

hey,
sorry to those of you who have been waiting for the timelapse. :(

i worked on it for a few days before i left on vacation, but couldn't get it right .... i think i'll just throw up the pics i took for it and you can scroll through them for a pseudo-timelapse experience ;)

the pretzel jar has been in the fridge inside a plastic bag for about 1.5 weeks now. i'm gonna dunk it tomorrow for a while .... 12 hrs maybe? not sure exactly. it's very large and dense, i imagine it needs a rather long dunk. then it will be set out on my countertop for (hopefully) a great 2nd flush.

#126 Nobody

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Posted 09 January 2011 - 06:26 PM

Very nice flush MD! Can't wait to see the second one... Have you cooked 'em up yet?

#127 mydarling

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Posted 10 January 2011 - 02:00 AM

"timelapse" time!

since i was using the camera to take a LOT of photos and not just the timelapse ones, these pics were all taken at different distances from the cluster. that's why the pics are all different sizes. i've been trying to use GIMP to layer and fit them all to compensate for this, but GIMP is giving me a migraine :gah:

so, i've cropped and resized them all so they're at approximately the same scale. so you can see the true change in size as the cluster grew. pardon the different sized pics.

so errrr, just scroll through them real fast and you can pretend it's an animated GIF! :D

(GIMP is soo hard to use, i'm still trying to figure it out, i'll try to get it worked out for the next flush....)

enough blabbering,
on with the pics!
 

1-360.JPG 2-400.JPG 3-400.JPG 4-425.JPG 5-407.JPG 6-402.JPG 7-472.JPG 8-535.JPG 9-595.JPG 10-670.JPG 11-750.JPG


Edited by Sidestreet, 13 January 2017 - 06:19 AM.


#128 ethnobotanist420

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Posted 10 January 2011 - 02:23 AM

awesome looking mushrooms! nice work on the time lapse photography also :eusa_clap

#129 mydarling

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Posted 15 January 2011 - 08:47 PM

2nd flush is coming in now,
but it's pretty disappointing.

the pins seem to only be forming on the top, in the dead space created between the substrate & the plastic when the substrate shrank after 1st flush.

they are also growing very slowly.

temps/FAE/misting regimen is the same as the first flush. i gave the whole thing a 12-13 hr dunk after it was taken out of the fridge, but it doesn't seem to have done much good here.

anyone have any insights?

i've read that oysters don't produce many flushes, but geez, i thought i could at least get 2! maybe i should cut the plastic away from the substrate and just fruit the giant block? i could fabricate an FC if needed, but i'd like to stay away from that if i can, as i know oysters prefer tons of FAE.

#130 mydarling

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Posted 15 January 2011 - 10:55 PM

ok, this may or may not be the best idea, but i so dearly want to help this giant substrate block!

figuring it is suffering because it needs more FAE and the plastic is stunting it,
i have decided to cut it out of the plastic jar and fruit it as a block!

i have placed it atop a wire cooling rack,
and then put a plastic bag over the top to maintain humidity.

the bag does not come in contact with the countertop (it's hooked around the edges of the rack), so fresh air can still get up into the bag through underneath the rack. but i'm also hoping that this plastic bag will help maintain some humidity for the pins! so ... if i am lucky ... this will allow for both FAE and humidity and perhaps i'll get a good pinset here.

i plan to take the plastic bag off to mist the block every couple of hours. it was given a good heavy misting before the bag was put on.

some pics:

wire rack.

pretzeljar-day53-01-resize750.JPG

plastic jar being removed.

pretzeljar-day53-02-resize750.JPG

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ye olde substrate block, free as a bird!

pretzeljar-day53-04-resize750.JPG

pretzeljar-day53-10-resize750.JPG

the wimpy little pins currently forming around the top edge.

pretzeljar-day53-06-resize750.JPG pretzeljar-day53-07-resize750.JPG pretzeljar-day53-08-resize750.JPG pretzeljar-day53-09-resize750.JPG

substrate block on wire rack, covered by plastic bag.

pretzeljar-day53-11-resize750.JPG

fingers crossed,
let's hope this turns out well,
i want to eat some oysters!!!! :loveeyes:

please feel free to provide any advice here on better ideas to make it fruit.


Edited by Sidestreet, 13 January 2017 - 06:21 AM.


#131 MrGumball

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Posted 17 January 2011 - 01:17 PM

What temp are they at now?

#132 mydarling

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Posted 17 January 2011 - 03:51 PM

it's around 70-75ish.
pretty warm, i know, but there's not much i can do about it.
they also fruited at these temps on the last flush, so it's odd they won't fruit now...especially after 1+ week cold shock and 12 hr dunk.

the plastic bag hasn't helped much, i'm taking it off now.
i do have a 5 gallon bucket with a bazillion holes drilled in it (used for grain prep), so i'm gonna give that a try and invert it over the top of the sub. with the wire rack still underneath. MAYBE this will give it enough FAE & humidity to make some damn fruits! :horse:

pic of bucket attached.

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#133 kcmoxtractor

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Posted 17 January 2011 - 04:55 PM

archive material

awesome photos MD!

#134 mydarling

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Posted 17 January 2011 - 10:26 PM

thanks kc! :love:

hope i get a 2nd flush out of this thing!
i know it has the potential, just a matter of coaxing those mushies out ........... :weedpoke:

i'm contemplating scraping the whole thing and giving it another dunk.

edit: ok i scraped it up with a scalpel, cut off all the aborted pins, sprayed the shit out of it, covered it w/ bucket full of holes ...... let's see what happens now.

Edited by mydarling, 17 January 2011 - 11:35 PM.


#135 mydarling

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Posted 19 January 2011 - 09:17 PM

:eusa_wall:eusa_wall:eusa_wall:eusa_wall:eusa_wall

nothing is happening!
totally completely stalled.

should i cold shock it again?
dunk it again?
tie the whole thing up in a plastic bag?
build an FC?
put it outside at night where it's much colder?
bury it in my vegetable garden?

:eusa_wall:eusa_wall:eusa_wall:eusa_wall:eusa_wall

#136 TastyBeverage

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Posted 19 January 2011 - 09:30 PM

I think putting it outside would help, and using the bag and rack system you had going there will help it to keep from drying out too much. You might want to cut some little holes in the top of the bag so that gravity can pull O2 down easier than convection action from below. Keep misting too. I think if you're just patient some will eventually pop. :)

#137 mydarling

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Posted 19 January 2011 - 09:34 PM

hi bev thanks for the advice :D do you think that using the bag with holes in it, is better than the 5 gal bucket which has a bunch of holes drilled in it? seems to me like they're doing the same thing, except the bucket is a lot easier to manage.

#138 roscoe

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Posted 19 January 2011 - 09:36 PM

if i had muh druthers i would pick the bucket, for oysters.:pirate:

#139 TastyBeverage

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Posted 19 January 2011 - 09:51 PM

Yar indeed. Me likes yon bucket. :pirate:

#140 mydarling

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Posted 19 January 2011 - 10:00 PM

okie dokie, i put it outside with the bucket in a shady spot .... i hope this works!




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