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lab sterile prints/clones/fruits at home, CHEAP :) merged


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#1 fahtster

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Posted 13 April 2006 - 10:42 AM

ok... i've been working on this tek for awhile now. basically i got tired of wondering if my prints were clean or not.
I had a pretty good printing tek before (just opening up a sterilized jar and throwing a cap in there).

 

well, i've figured out how to get completely sterile prints at home for cheap. (completely sterile is a pretty bold statement, so i'll call it as close to sterile as you can get without a glovebox, flowhood, or a lab at home).

This tek is not complete at this moment as i don't for some reason have access to a stove to pc anything.. hopefully that will change in the next couple of days. damn gas company..

 

anyway, the only thing that i can see changing is the materials some things may need to be switched out for something else.
I'm a little worried about the cable zip ties.. but they are a 4 rating for the plastic, so they should work ok. but the process itself is sound. :)

 

Overview: this tek is using an oven bag to colonize, fruiting out (even with a casing) and print without even opening the oven bag. :) basically, I'm sectioning off the oven bag with zip ties until whatever is in that section is needed. corn in one section... verm. in another... and the jar for printing in the last section. I'm also introducing an addition to hippies airport tek. it's called the airbag. lol you'll see. ;) ok... with that said, here we go...

 

1. making the bag. here is a picture of the bag after the gas exchange ports and innoc. ports have been added... there is also a main airport at the top of the bag..

 

emptybag.JPG

 

the gas ports and innoc ports are made from 5/16 i.d. x 1/2 o.d. tubing...

 

liltube.JPG

 

then a hole smaller than the diameter of the tubing is made in the oven bag and the sides of the oven bag are pulled almost to the top of the tubing. Then two pieces of tyvek are wrapped over the top of both the oven bag and tubing, then zip tied with a cable zip tie as tight as it can possibly go...

 

nozonbag.JPG

 

you can see in the first picture that only one side has two ports.. this is because thats where the corn goes. one port is for the innoculation which is then just taped up ( I ALWAYS wipe the glue side of the tape off before i swiftly put it over the innoc hole with the needle still in it, then slide the needle out while pushing down on the tape.. it's a good practice folks ;) ).
the airport is just a 2.5 inch piece of 5/8 i.d. x 7/8 o.d. tubing stuffed with polyfil. this acts just like hippies syringe airport and even better with the airbag tech.

 

airportalone.JPG

 

the airport is attached in the same fashion as the gas ports and innoc. ports but without the tyvek...

 

airportonbag.JPG


Edited by Sidestreet, 05 October 2016 - 04:34 AM.


#2 fahtster

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Posted 13 April 2006 - 10:46 AM

2. filling the bag. now put a few handfuls of corn into the corner of the bag that has the two ports described earlier...

corninbag.JPG

now zip tie off the corn from the rest of the bag... be sure to leave enough room to knead the corn later for faster colonization...

corntwist.JPG

now put your casing material into the other corner of the bag with the one gas port. this port is important because the verm could get bacteria if there isn't at least a little bit of gas exchange...

verminbag.JPG

twist and zip tie off the verm. like you did for the corn...

vermtwist.JPG

this is where the real magic happens.. put a clean 1/2 pint jar covered with two layers of tyvek (ink side up) and band (LOOSELY on) into the third main chamber. I put a piece of broken glass on the bottom to help scrape the spores off into the water.

jarinbag.JPG


Edited by Sidestreet, 05 October 2016 - 04:35 AM.


#3 fahtster

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Posted 13 April 2006 - 10:50 AM

zip tie off the main opening to the bag now.

jartwist.JPG

put foil over the airport opening so water doesn't drip in there.

foil.JPG

3. pressure cook and innoculate. now pc this at 15 psi for 45 min. and let cool.
once cool, take out of pc and innoc. the corn thru one of the ports... remember, clean tape.
cover the tyvek on the innoc port entirely.

now wait for the corn to colonize. once the corn gets a nice patch of myc.,
knead it to spread the myc. throughout the rest of the corn for fast colonization times.
once the corn is colonized, get the scissors ready..

scissors.JPG

4. casing and fruiting. FROM THIS POINT ON, PRETEND THE CORN IS COLONIZED.. hehe. cut thru the 'head' of the zip tie (BE VERY CAREFUL NOT TO CUT ANY PART OF THE OVEN BAG DOING THIS) that is separating the corn from the main chamber (the one with the jar in it).

uncut.JPG

move the jar as far away from the corn as you can and put a piece of foil under the oven bag where the corn is sitting..

foilunder.JPG


Edited by Sidestreet, 05 October 2016 - 04:44 AM.


#4 fahtster

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Posted 13 April 2006 - 10:56 AM

now curl the sides of the foil up to encase the corn... remember the foil is OUTSIDE of the oven bag.. the bag doesn't get opened until the print is ready to come out. the foil will keep light off the bottom of the corn preventing pinning there. you can use anything you want.. i.e. a bowl or dish but foil should work fine. the cheaper the better.

foilbowl.JPG

now cut the zip tie that is separating the verm. from the now one main chamber..

vermcut.JPG

vermabouttocase.JPG

now here is why the oven bag is nice... you can use your hands on the outside to manipulate things on the inside. :) use your fingers to move the verm. over the corn. tip: if you think the verm. has dried out too much, just add sterile water via syringe thru the verm. gas port and cover with clean tape afterwards. this tek is self humidifying so make sure the verm layer is nice and wet.

corncased.JPG

 


Edited by Sidestreet, 05 October 2016 - 04:47 AM.


#5 fahtster

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Posted 13 April 2006 - 11:03 AM

5. The Airbag. ok yall... here it is. i was having trouble finding a way to get fresh air pushed in thru the polyfil and into the fruiting chamber.. a syringe would take forever and a day. then i thought... well.. what if i transfered air from one bag to another via squeezing! i got very excited.. cuz it works! check it out... the airbag itself is a new kitchen bag filled with air from the room and then tied off at the top... then a small hole is put in it and quickly zip tied onto the airport
at the top of the bag...
airbag.JPG
airbagconnect.JPG
a closer look at the connection...
airbagconnectclose.JPG
in this pic the air has been squeezed out of the airbag and into the fruiting chamber..
deflateairbag.JPG
the airbag is then removed and the chamber is left full of fresh air. :)
inflatedFC.JPG
heres a top view..
FCtopview.JPG


Edited by Sidestreet, 05 October 2016 - 04:50 AM.


#6 fahtster

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Posted 13 April 2006 - 11:07 AM

getting the air in was easy, but what about getting it out... well... thats even easier... just suck the shit out. lol put a piece of paper towel over the airport (don't want to inhale those polyfil 'bits') and suck.. easy as that.
suckdry1.JPG
sucking.JPG
heres a pic of the chamber after i sucked out the old air...
suckeddry.JPG
6. Printing. ok... so now you have healthy fruits and you are ready to print a cap. just pinch (thru the bag still) a cap off a stem using your thumb and the jar rim, 'popping' the cap into the jar for printing... once the print has been dropped... dump out the cap and screw on the lid. heres a pic were i screwed the lid off thru the bag.. it wasn't that hard as long as the lid was on there loosely...
lidoff2.JPG
NOW you can open the oven bag and retrieve your jar with sterile print. :) OR you can print as many of the caps into that one jar as you want. because you used tyvek.. you can pull spores from there whenever you want and no spores will be lost as any left over spore water in the jar will evaporate thru the tyvek. :) just be sure to retape any holes you put in the tyvek... again.. clean tape.
this has many uses as i see it... if you don't like how big the oven bag is... well.. adjust it.. just pull as much bag as you don't want to use and zip tie it.. that simple. you can also clone with this... just use a 'faht jar' instead of an empty jar and break off a piece of desired stem into the faht jar before sporalation begins (or you may get more substrains in your iso jar). you could also put a bunch of grain in one whole bag and your casing in another and connect them using large tubing... just block off one side of the tubing with foil when you make the bags. then pc them both together... when you are ready to use your casing... just break the foil seal and pour your casing on thru the large diameter tubing. :) hope this helps at least one person.. stayed up all night writing it. lol ;)
fahtster


Edited by Sidestreet, 05 October 2016 - 04:51 AM.


#7 fahtster

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Posted 13 April 2006 - 11:07 AM

there. :)

fahtster

#8 Elf Salvation

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Posted 13 April 2006 - 11:10 AM

Very inventive as allways Fahtster


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#9 CY81N

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Posted 13 April 2006 - 11:12 AM

Great innovative tec fahtster!!

#10 Guest_freakachino_*

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Posted 13 April 2006 - 11:12 AM

:) :cool:

Great write up fahtster!!! :bow: Pics are great!

I love the simplicity and sterility of this. You really did a great job of thinking up an easy system!!!! I like the concept a lot! The air bag makes a great addition for the bag also.

#11 Beast

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Posted 13 April 2006 - 11:16 AM

Wow, good thinking! I know where this will be heading!
:bow:

#12 fahtster

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Posted 13 April 2006 - 11:24 AM

i know this would be better if it was all flushed out and everything... thats still to come... i have to get the gas company to come and hook up my new stove before i can start anything... they are being bitches about it. came twice already, both times it was different peeps.. and they both brought the wrong damn parts. ugh.. so... it'll continue to the finish soon, hopefully. :)

fahtster

thanks for the kind words yall. :)

#13 fahtster

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Posted 13 April 2006 - 11:33 AM

just wanted to add how i prep the grain also...
First, put your corn in the pc without your jar rack (the plate the pc comes with to keep the jars off the bottom of the pc.) and cover with at least three inches of water.
you can probably go a little less once you get the hang of how much the dry kernels will absorb. on average the kernel will expand 3 times it's size.
Now pc at 15 psi for 1 hour. after an hour, turn off the pc and left the pressure adjust back to zero... once the safety nob drops, you can unscrew the lid. be very careful.. the pc will still be very hot. don't let the steam get ya in the face.
Now pour the corn into a strainer. I rinse off the corn with hot water first. the kernels will have lots of starch on them.. if you rinse with cold water first the starch will coegulate making it a bitch to get off. rinsing is a very important part of the process... you want to get the kernels as free from starch as possible. once you've rinsed with hot water... rinse with cold. this will cool the kernels down making them easier to handle and also 'tighten' the kernel up making it not so mooshy.
once rinsed, i let the corn sit for a few minutes to drain. Next i lay down a piece of paper towel and lay the corn on top of that in a single kernel layer. then i place another piece of paper towel on top of the corn layer and roll my hands lightly over the corn. this will wipe off excess water and starch from the corn before i place it in the bag. :)

faht

#14 Guest_cap_*

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Posted 13 April 2006 - 11:37 AM

you, or hippie or someone should market this sack o shroomage to new growers for a modest fee:D

$$$cha chinG :D

#15 fahtster

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Posted 13 April 2006 - 05:33 PM

lol... i did think for a bit about selling it.. but decided to say fuck it. at the moment the tek is incomplete but i have high hopes.. probably needs tweaking here and there but for the most part i think it's pretty sound. like i said.. i'm a little worried about the zip ties and possibly having a bag that is leaky.. that kind of thing. but i think that it's definately worth the post. :)

fahtster

#16 Hippie3

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Posted 13 April 2006 - 05:45 PM

it's a 'kit'.
cool
nice to see someone pursuing this line of research.
:cool:
looking forward to seeing it in action.

#17 malefacter

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Posted 13 April 2006 - 06:00 PM

i know this would be better if it was all flushed out and everything... thats still to come... i have to get the gas company to come and hook up my new stove before i can start anything... they are being bitches about it. came twice already, both times it was different peeps.. and they both brought the wrong damn parts. ugh.. so... it'll continue to the finish soon, hopefully. :)

fahtster

thanks for the kind words yall. :)

hook it up yourself

#18 fahtster

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Posted 13 April 2006 - 06:11 PM

i can't... if you don't let the gas company hook it up and the house burns down, you don't get insurance

#19 Hippie3

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Posted 13 April 2006 - 06:25 PM

people offer bad advice pretty freely eh.
tampering with the utilities is a good way
to draw massive heat that you don't need.

#20 BuckarooBanzai

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Posted 13 April 2006 - 08:15 PM

Wow, dude, that is a killer idea. I can't say my FOAF would ever use it directly, but I can say he sees at least one innovation there which he may well abscond with!

Excellent write up and pics, too. Really top notch work!




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