ok... i've been working on this tek for awhile now. basically i got tired of wondering if my prints were clean or not.
I had a pretty good printing tek before (just opening up a sterilized jar and throwing a cap in there).
well, i've figured out how to get completely sterile prints at home for cheap. (completely sterile is a pretty bold statement, so i'll call it as close to sterile as you can get without a glovebox, flowhood, or a lab at home).
This tek is not complete at this moment as i don't for some reason have access to a stove to pc anything.. hopefully that will change in the next couple of days. damn gas company..
anyway, the only thing that i can see changing is the materials some things may need to be switched out for something else.
I'm a little worried about the cable zip ties.. but they are a 4 rating for the plastic, so they should work ok. but the process itself is sound. :)
Overview: this tek is using an oven bag to colonize, fruiting out (even with a casing) and print without even opening the oven bag. :) basically, I'm sectioning off the oven bag with zip ties until whatever is in that section is needed. corn in one section... verm. in another... and the jar for printing in the last section. I'm also introducing an addition to hippies airport tek. it's called the airbag. lol you'll see. ;) ok... with that said, here we go...
1. making the bag. here is a picture of the bag after the gas exchange ports and innoc. ports have been added... there is also a main airport at the top of the bag..
the gas ports and innoc ports are made from 5/16 i.d. x 1/2 o.d. tubing...
then a hole smaller than the diameter of the tubing is made in the oven bag and the sides of the oven bag are pulled almost to the top of the tubing. Then two pieces of tyvek are wrapped over the top of both the oven bag and tubing, then zip tied with a cable zip tie as tight as it can possibly go...
you can see in the first picture that only one side has two ports.. this is because thats where the corn goes. one port is for the innoculation which is then just taped up ( I ALWAYS wipe the glue side of the tape off before i swiftly put it over the innoc hole with the needle still in it, then slide the needle out while pushing down on the tape.. it's a good practice folks ;) ).
the airport is just a 2.5 inch piece of 5/8 i.d. x 7/8 o.d. tubing stuffed with polyfil. this acts just like hippies syringe airport and even better with the airbag tech.
the airport is attached in the same fashion as the gas ports and innoc. ports but without the tyvek...
Edited by Sidestreet, 05 October 2016 - 04:34 AM.