Lazlo:
If you fail and really want this strain I can easily do a spore extraction from a dried gill and get it back to you. I've done it successfully dozens of times, even with herbarium specimens and specimens crush to dust in the mail.
Psilocybe West Virginian specimens....
Started By
Lazlo
, May 02 2006 12:04 AM
141 replies to this topic
#22
Lazlo
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old hand
Posted 17 May 2006 - 10:05 PM
The tissue sample was apparently not a good one. I'm running away from bacteria on agar. Hopefully this transfer will work out. The amount of visible mushroom mycelium was very small. Just enough on top of the tissue to scrape off for tranfer. So the transfer was very hard with the unsteady hands I have. A small amount of the stem was refridgerated in case of failure.
I'll send you a sample Workman if needed.
I'll send you a sample Workman if needed.
#24
SporeAddik1
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Mycophage
Posted 17 May 2006 - 11:42 PM
I would do a gill transfer, thats probably your best bet, like i_am_me said, however it sucks if you can't get an actual clone. GOOD LUCK MAN!
#25
Lazlo
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old hand
Posted 18 May 2006 - 06:50 AM
I made a new plate last night with 2 samples.
#26
apokalypse
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Dr. Durgs
Posted 18 May 2006 - 07:30 AM
It'll be interesting to see what this turns out to be.. are you going to have someone analyze it under a scope? Best of luck!
#28
Guest_dial8_*
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Posted 18 May 2006 - 09:00 AM
This is cool. Workman, if you get a sample will you please post your technique here?
#29
Hippie3
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DUNG DEALER
Posted 18 May 2006 - 11:05 AM
I made a new plate last night with 2 samples.
:eusa_pray
#30
Guest_freakachino_*
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Posted 18 May 2006 - 11:58 AM
Good Luck with the dishes Lazlo! Hope they grow well for you :)
#31
Lazlo
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old hand
Posted 19 May 2006 - 01:44 PM
Here are the 2 dishes. The first dish is the transfer dish. As you can see the transfer was very sloppy from the size of it. I knew bacteria would be transfered as well, so I swabbed a 1/2 dollar sized shot of 3% H202 on the inoculation point to see if it would help out. It appears to be helping a lot. At first sight I thought the transfer was doomed because it looked as if bacteria was spreading from the transfer, but it's whitening up and spreading out nicely.http://mycotopia.net/forums/attachment.php?attachmentid=26045&stc=1&d=1148063775
This is the new plate with 2 inoculations. It's hard to see but, they appear to be a lot cleaner than my first attempt. It looks as if i'll have plenty of good mushroom mycelium to work with now. http://mycotopia.net...=1&d=1148063775
This is the new plate with 2 inoculations. It's hard to see but, they appear to be a lot cleaner than my first attempt. It looks as if i'll have plenty of good mushroom mycelium to work with now. http://mycotopia.net...=1&d=1148063775
Attached Thumbnails
#34
bullfrog
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Mycophage
Posted 15 July 2006 - 12:18 AM
in john allen's site [i think] , there is writings of mushroom
articles on a page . anyway it tells of a place in wva where
people got in trouble when they got caught picking native ps
cubies. i'll look for the article and post it. i hope you got the
strain. good luck man. bllfrg
articles on a page . anyway it tells of a place in wva where
people got in trouble when they got caught picking native ps
cubies. i'll look for the article and post it. i hope you got the
strain. good luck man. bllfrg
#35
bullfrog
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Mycophage
Posted 15 July 2006 - 12:33 AM
Intelligencer & Wheeling News-Register
Wed, 15 May 2002
ILLEGAL MUSHROOM PICKING LEADS TO ARRESTS
by
John Phillips
Approximately 50 people have been arrested in connection with picking of psilocybin mushrooms near Castleman's Run Road in Brooke County near Bethany.
Brooke County Sheriff Bernie Kazienko confirmed Tuesday the arrests have been made since May 1, and 30 more people face charges of picking the psilocybin mushroom. Kazienko explained that the psilocybin mushroom, when ingested, produces a mild hallucinogenic effect or "buzz."
Psilocybin mushrooms are classified as a controlled substance under the West Virginia Controlled Substance Act.
"This is an annual event during the mushroom season, which is May through September," Kazienko explained.
"The last couple of years, we've been watching the area, and watching which mushrooms have been picked."
He said that while the arrests have been made during the initial investigation, many suspects also were charged with possession of marijuana either on their person or in vehicles. Some individuals attempted to escape the area and were charged with fleeing from police.
"We've actually come in contact with approximately 100 people, and other charges are pending on close to a total of 80 individuals," Kazienko said. "There are also juveniles involved."
He said the property is owned by Wheeling-Pittsburgh Steel Corp. and that the steel company has been cooperative.
He also said he met with an environmentalist and an attorney from Wheeling-Pitt, which owns 160 acres in the area.
"We took them to the scene," Kazienko said, adding that since the meeting, Wheeling-Pitt has contacted the West Virginia University Extension Office and the West Virginia Department of Natural Resources.
"They wanted to see what they could do to eradicate the mushrooms," Kazienko said.
He said there are other options, such as using a bulldozer to clear the area.
"There will be costs for Wheeling-Pitt associated with that," Kazienko said.
Wheeling-Pitt has since hired security personnel to patrol the area. Bethany College security also is involved in the patrolling.
"We're going to have people indicted by the grand jury on June 3 in connection with the mushroom incidents," Kazienko said. "Some have been arrested for possession with intent to deliver the mushrooms and others for possession with intent to deliver marijuana."
Brooke County Sheriff's Deputy Steve Seminsky also has been working on the investigation and confirmed more warrants will be issued. He said some plain clothes officers also have been on patrol.
"We're learning this has been going on for several years," Seminsky said. "We've found people out there from the break of day to late evenings, just after dark." Seminsky said, explaining that none of those arrested had to be transported to the Brooke County Jail in Wellsburg, but were processed at the scene.
"Our biggest concern is the safety of the individuals," Seminsky added. "We don't want them ODing on the mushroom or taking the wrong mushroom and dying."
Charges were were logged against people from Ohio, West Virginia and Pennsylvania.
A person arrested for simple possession faces a misdemeanor charge and the penalties can include 90 days to six months in jail, a fine of up to $1,000, or both. Persons also may be placed on probation for six months.
Persons found in possession of the mushrooms with intent to deliver or sell face a felony charge and that carries a prison term of one to five years, a fine of up to $15,000, or both. In addition, any property connected to the incident may be seized, pending forfeiture through the court system.
Return to the Grape Vine Index
Retrn to Main Index
from mushroom john's site
Wed, 15 May 2002
ILLEGAL MUSHROOM PICKING LEADS TO ARRESTS
by
John Phillips
Approximately 50 people have been arrested in connection with picking of psilocybin mushrooms near Castleman's Run Road in Brooke County near Bethany.
Brooke County Sheriff Bernie Kazienko confirmed Tuesday the arrests have been made since May 1, and 30 more people face charges of picking the psilocybin mushroom. Kazienko explained that the psilocybin mushroom, when ingested, produces a mild hallucinogenic effect or "buzz."
Psilocybin mushrooms are classified as a controlled substance under the West Virginia Controlled Substance Act.
"This is an annual event during the mushroom season, which is May through September," Kazienko explained.
"The last couple of years, we've been watching the area, and watching which mushrooms have been picked."
He said that while the arrests have been made during the initial investigation, many suspects also were charged with possession of marijuana either on their person or in vehicles. Some individuals attempted to escape the area and were charged with fleeing from police.
"We've actually come in contact with approximately 100 people, and other charges are pending on close to a total of 80 individuals," Kazienko said. "There are also juveniles involved."
He said the property is owned by Wheeling-Pittsburgh Steel Corp. and that the steel company has been cooperative.
He also said he met with an environmentalist and an attorney from Wheeling-Pitt, which owns 160 acres in the area.
"We took them to the scene," Kazienko said, adding that since the meeting, Wheeling-Pitt has contacted the West Virginia University Extension Office and the West Virginia Department of Natural Resources.
"They wanted to see what they could do to eradicate the mushrooms," Kazienko said.
He said there are other options, such as using a bulldozer to clear the area.
"There will be costs for Wheeling-Pitt associated with that," Kazienko said.
Wheeling-Pitt has since hired security personnel to patrol the area. Bethany College security also is involved in the patrolling.
"We're going to have people indicted by the grand jury on June 3 in connection with the mushroom incidents," Kazienko said. "Some have been arrested for possession with intent to deliver the mushrooms and others for possession with intent to deliver marijuana."
Brooke County Sheriff's Deputy Steve Seminsky also has been working on the investigation and confirmed more warrants will be issued. He said some plain clothes officers also have been on patrol.
"We're learning this has been going on for several years," Seminsky said. "We've found people out there from the break of day to late evenings, just after dark." Seminsky said, explaining that none of those arrested had to be transported to the Brooke County Jail in Wellsburg, but were processed at the scene.
"Our biggest concern is the safety of the individuals," Seminsky added. "We don't want them ODing on the mushroom or taking the wrong mushroom and dying."
Charges were were logged against people from Ohio, West Virginia and Pennsylvania.
A person arrested for simple possession faces a misdemeanor charge and the penalties can include 90 days to six months in jail, a fine of up to $1,000, or both. Persons also may be placed on probation for six months.
Persons found in possession of the mushrooms with intent to deliver or sell face a felony charge and that carries a prison term of one to five years, a fine of up to $15,000, or both. In addition, any property connected to the incident may be seized, pending forfeiture through the court system.
Return to the Grape Vine Index
Retrn to Main Index
from mushroom john's site
#36
Lazlo
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old hand
Posted 15 July 2006 - 04:37 AM
This place consists of around 9000 acres. This story isn't affiliated with the area these came from.
What's up Bullfrog? :) I'm not familiar with this area though. I'll check it out. Thanks.
The culture is on ice for now, but ready to go.
What's up Bullfrog? :) I'm not familiar with this area though. I'll check it out. Thanks.
The culture is on ice for now, but ready to go.
#37
bullfrog
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Mycophage
Posted 15 July 2006 - 08:47 AM
howdy lazlo,
how's it going? good i hope . all is well here. i have some new shiitake cultures , if ya' want one.
how's it going? good i hope . all is well here. i have some new shiitake cultures , if ya' want one.
#38
golly
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modapotato
Posted 15 July 2006 - 09:28 AM
So they wanna bulldoze an entire habitat...how fucked up is that...
Hey Laz...your sitting on the find of the 21st century man -u gotta grow em out...:amazed: I'm just messin with ya, but i would love to see em go pop..
Hey Laz...your sitting on the find of the 21st century man -u gotta grow em out...:amazed: I'm just messin with ya, but i would love to see em go pop..
#39
Lazlo
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old hand
Posted 15 July 2006 - 01:53 PM
howdy lazlo,
how's it going? good i hope . all is well here. i have some new shiitake cultures , if ya' want one.
I've got some, but thanks for the offer man.
I'll start to attempt fruitings soon.
#40
Lazlo
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old hand
Posted 15 July 2006 - 01:58 PM
Oh Lord! They're going to bulldoze the place? lol! Just spray the plot with a fungicide or something. Idiots.
