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potato water?


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#1 chill

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Posted 08 May 2005 - 09:28 PM

Hi,

I have had almost no luck with liquid cultures what so ever.

I am using two jars with 15 and 30% potatoe water / tap water solution. Do these photos show mycelium growth or is this just the starch clumping up?

I'm especially hoping for mycelium as this could be my only shot at pan cyans as the syringes I made from a print produced only one pf jar.

Attached Thumbnails

  • potatoe_water 001-1.JPG
  • potatoe_water 004-1.JPG


#2 chill

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Posted 08 May 2005 - 09:29 PM

BTW, I forgot to mention that the jars were shot up a week ago today if that makes any difference.

#3 destroy_erase_improve

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Posted 08 May 2005 - 09:41 PM

im far from a liquid culture expert, but that looks like myc collecting together to me. shake it up and it should break apart a little into strands. if so, suck some up and do a test jar.

#4 Alkady Mycomania

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Posted 09 May 2005 - 01:04 AM

That looks mycelium clumps in the first picture especially... my potato water jar of oysters looks just like that, except clearer (12% potato water solution). I don't really know what is in that second pic, but I'm not vastly experienced in liquid culture either.
Try inoculating a test jar with that stuff to test for contams.

Is this from spores or from mycelium/mushroom tissue?

#5 chill

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Posted 09 May 2005 - 11:08 AM

This is from a home made syringe of pan cyan spores.

I only have time to work on this hobby on the weekends really so I guess I'll do a couple of jars on Saturday and shoot them up.

#6 Hippie3

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Posted 09 May 2005 - 11:15 AM

looks like tissue to me
but let it grow more

#7 chill

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Posted 09 May 2005 - 12:18 PM

Hi Hip,

This was inspired by your write up of potatoe water in serial solution (50, 25, 12 and 6%).

I thought that mycelium floated (like in your write-up) yet this stuff is just lounging around on the bottom. Will it float at some point or can mycelium do both?

#8 Hippie3

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Posted 09 May 2005 - 12:40 PM

it can do either
as the specific density of the solution can vary.

#9 anticheffy

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Posted 09 May 2005 - 12:52 PM

yea, the potatoe water I have done always gels after a few weeks of growth, dont know why , grows both on top and/or in the solution

strange

#10 TripTout

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Posted 09 May 2005 - 01:16 PM

Light corn syrup inoculant always tend to sink for me.

#11 Filamentous Fungi

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Posted 09 May 2005 - 01:20 PM

looks like culture...put it on an electric back massager if you dont have a vortex and you will get a really strong high cell culture which, if done right will lead to even colonization of jars if you are using grain and shake the grain after innoculating.

#12 chill

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Posted 09 May 2005 - 06:01 PM

looks like culture...put it on an electric back massager if you dont have a vortex ...


Thanks Fil,

What is a vortex?

#13 puppydog23

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Posted 03 May 2006 - 06:20 PM

hey, ive been combing the vaults for how to make potato water and cant seem to find a tek.... anyone care to help me out? puhleeeeeze?

#14 Hippie3

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Posted 03 May 2006 - 08:24 PM

take about 1 pound of potatoes
Wash the potatoes but don't peel. Slice into small pieces.
Cook potatoes until tender, in about a pint of water.
Strain and save the liquid. Discard the potatoes or save to eat later.

#15 Antranik

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Posted 03 May 2006 - 11:00 PM

Is the potato water something to use instead of spring water?

#16 Hippie3

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Posted 03 May 2006 - 11:26 PM

it's used for agar and liquid cultures, mostly

#17 puppydog23

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Posted 04 May 2006 - 05:53 AM

thanks a bunch hip, ur the man! im gonna give that a whirl and see if i dont like it better than karo

#18 Guest_lost_onabbey_rd_*

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Posted 04 May 2006 - 11:42 AM

just make sure to strain it real well.. i used 2 layers of paper towels and it was still a bit cloudy.. but atleast you don't have to worry about carmelizing any sugars
good luck
let us know how it turns out
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#19 puppydog23

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Posted 04 May 2006 - 07:20 PM

thanks for the tip, i was kinda wondering about that, how much i should filter it and all, hopefully before too long it will be cloudy with myc!

#20 puppydog23

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Posted 08 June 2006 - 09:24 PM

ok, so i seem to have some growth on the bottom of my potato water, looks like thick white stuff, but the water is pretty cloudy, ive been stirring, nocd like 4 days ago, how long should i wait till i use it to noc up some jars?




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