fractional sterilization [merged]
Posted 30 April 2005 - 05:04 PM
First, steam the material for 30 minutes. Incubate at 37C for 12 to 24 hours to allow bacterial germination. Repeat steaming and incubation steps, and then steam again for 30 minutes after the second incubation period.
That's it. Hopefully someone finds this useful.
Posted 30 April 2005 - 06:32 PM
Believe it is also called Tindilization as well.
Posted 07 May 2005 - 07:04 AM
and i've been told to steam
three times on three successive days
Posted 07 November 2005 - 01:30 AM
Here is what I did: I took the WBS I was going to use, put in a bowl and put in 200ml of bleach with every 3 cups of water and let the seed soak stirring occasionaly for about an hour. Then I drained and rinsed the seed. Next I brought the seed to a slow boil in a pot of water, and when it started boiling I let it do this for 10 mins. Afterwards, I drained and loaded into some jars, and sterilized by boiling for an hour and a half. Let them cool and then innoculated with an LC.
That was just a few days ago and the growth is looking pretty good and so far no sign of contams. I did this to avoid fractional sterilization. So far so good! Anyone tried this before? Any comments?
Posted 07 November 2005 - 05:01 AM
agar at the shroomery said he got growth on ground WBS PF cakes that were microwaved for only 4-6minutes.
Posted 07 November 2005 - 01:10 PM
Posted 07 November 2005 - 01:40 PM
The bleach may be able to penetrate the small WBS grains.
Posted 07 November 2005 - 02:03 PM
Posted 07 November 2005 - 02:08 PM
the best is RGS except it is so damn expensive.
Posted 07 November 2005 - 02:20 PM
Posted 08 November 2005 - 01:59 AM
Posted 08 November 2005 - 02:17 AM
Posted 08 November 2005 - 05:06 AM
That is strange. I had heard it had a high endospore count. Yet many say they get away with pasteurising straw which has heads in it. Those straw heads look like RGS.
RGS was another I figured would have a good chance. And I think I read somewhere that RGS is better at not growing contams than some other grains.
I like RGS for fractional sterilization in the microwave. It does not explode when heated in excess water like rye/wheat etc. So you can cover it totally in water and heat it for a long time in the microwave driving off water as you go. The excess water leads to an even heating with no hot/cold spots in the jar, since water is so conductive. I have microwaved it down until it was completely dry again, then added moisture back as LC. This will lead to rapid colonisation with no need for shaking of the jars, leaving little time for any contams to take hold.
Posted 09 November 2005 - 02:43 AM
If anyone has given this a shot, I would strongly suggest the boiling after the bleach soak. I tried some jars with a bleach soak and rinse and then to the jars and I think the bleach killed the tissue from my LC because I have yet to see anything good or bad. Could have not rinsed long enough though.
Sorry I cannot post pics. My birthday is on the 17th of this month, and I think my chick got me a new camera (been hinting for a while)!! Hope so cause I want to post my successes on a few projects I have going, like this one.
Posted 09 November 2005 - 05:48 AM
This bleaching would probably work well with RGS with its thin open structure.
Posted 12 November 2005 - 03:37 PM
Posted 16 May 2006 - 09:04 PM
If a person were to get thick plastic and an iron with a towel and make a Myco bag type thing. I'm talking a Myco bag that eats other myco bags for dinner and shits ethenal.
One that would fit 50lbs of popcorn.
All done in a 55 gallon drum of course. You would need a cherry picker to hang it, and a floor jack to kneed it.
But could it work?
Or, some 55 gallon drums have a metal ring that seals the cover on tight. Could a person add a pressure guage to it and call it a 220 qt home made pressure cooker.
Or one heck of a home made Myco-bomb, one of the two.
You could use a shovel...
Build Mount' Spawn, and put a little mycotopia flag on top.
Posted 16 May 2006 - 10:32 PM
Posted 16 May 2006 - 10:37 PM
yeah a diy PC is not a good idea