gloveboxes for cloning ?
Posted 18 May 2006 - 01:46 AM
Posted 18 May 2006 - 06:12 AM
Posted 18 May 2006 - 11:55 AM
Posted 18 May 2006 - 02:29 PM
Posted 18 May 2006 - 05:41 PM
Posted 18 May 2006 - 11:32 PM
Posted 18 May 2006 - 11:36 PM
Posted 19 May 2006 - 12:22 AM
I have a glovebox specifically for grain to grain, working on open lc's and a few other sterile procedures..
The beginning grower can easily cultivate without using a glovebox.
syringes can be made by using the ziplock bag method, place the print in bag... inject sterile water with a syringe and suck it back up into the syringe... easy as pie.
innoculation can be done w/ a very low contam rate by simply keeping your area clean and cutting off all air circulation if possible. Innoc in a small empty room if you can like a closet or bathroom, always wear clean clothes, use oust or lysol for the air sanitization, put some ISO alcohol in a spray bottle and add some water to dilute and you can use that to spray down all surfaces. IMO all open air procedures just come down to cleanliness.
Posted 19 May 2006 - 01:44 AM
Posted 19 May 2006 - 02:44 AM
Posted 19 May 2006 - 09:21 AM
Posted 19 May 2006 - 05:12 PM
spray the glove box with a mixture with bleach and water (all materils aready in there...the mushroom cap in there cleaned with h202 and a wine glass pc'd), let the mushroom cap sporlate in the wine glass for a day, then use sterilised syringe to inject water into the wine glass and use needle tip to seperate spores from the glass. then i will leave it in there for 24 hours (to let the spores soak) then i will innoc 4 jars and one jar of lc, i will do this 3 times, incase that if one batch of innoc gets contamed not all my work would be for nothing. this will take around 3 days and seems alot of work for just making syringes. but becoming self sufficent is the first step right.
tell me if anythings wrong with this. i might substitute the wine glass for something else, but i think the shape of the glass helps the spores to drop to the bottom, so when i mix it with the syringe needle more spores will be in the syringe in the end.
then i might birth the 6 best out of the 12 jars and let the other do what they wish. after the first flush i will use put all the cakes in the glove box, and make a big flat cake and case it with verm and let it fruit.
Posted 19 May 2006 - 05:21 PM
Posted 19 May 2006 - 05:29 PM
Posted 19 May 2006 - 05:44 PM
Posted 20 May 2006 - 05:06 AM
Posted 20 May 2006 - 07:44 AM
Try using cardboard. Cut a disk out and take one layer of card off so it is corrigated and scrape leftover glue off. I use a small jamjar with a lid with a drilled hole with tyvek glued over. A shallow jar makes life easy for later scalpel work. PC the jar with wet card disk for 20 mins..
Take a fresh mushie, wipe with alcohol, in glovebox tear open and cut a virgin untouched piece from inside with sterile scalpel. Cap or stem no matter. And it takes like agar.. The rhizo's run like agar, its works so well. thanks to whoever wrote the tek.
I've got button and oyster clones at the moment from store bought shrooms...
Works well to clone off your best cluster of a multispore flush, then use that as a master. Yeilds go up considerably...
Posted 20 May 2006 - 07:46 AM
- mycotopia77 likes this