The larger the tissue sample can increase mistakes as well. Stay small with the sample and retirieve it from a tight spot from within the fruitbody.
gloveboxes for cloning ?
Started By
night_ryder
, May 18 2006 01:46 AM
47 replies to this topic
#42
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Posted 21 May 2006 - 07:38 PM
in a petri dish?Well actually from what Ive observed it does appear to do just that, cannibalize the tissue.
It could be that the mycelium is able to utilize the moisture from the tissue with less effort than absorbing it from the cardboard. But in my initial experiements using small samples, about the size of rice grains, the carboard would not only grow fairly slow, but also would stop growing after colonizing about 30% of a 1' piece of cardboard. Also only one side of the cardboard would get colonized.
When using a larger sample, the growth would be MUCH faster(due to the surface area Im sure), and the entire piece of cardboard would colonize front AND back
#43
beebopboy
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Posted 21 May 2006 - 07:58 PM
4 out of 10 were in petris, the rest were in plastic baggies.
The first 5 I did were all small samples with 3 being in petris. The next 5 were all mainly in plastic baggies except for one in a petri.
The first 5 I did were all small samples with 3 being in petris. The next 5 were all mainly in plastic baggies except for one in a petri.
#44
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Posted 21 May 2006 - 08:07 PM
what would you say the benefits/downsides are of each ?
which did you like better and why ?
also, how did you sterilize the baggie ?
molto grazie:bow:
which did you like better and why ?
also, how did you sterilize the baggie ?
molto grazie:bow:
#45
beebopboy
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Posted 21 May 2006 - 10:12 PM
I liked to use the baggies myself because they seem to keep in more moisture for a longer period of time
Theres no need to sterilize the baggies since
1.) contams wont grow on the cardboard
2.) baggies are sterile out the box.
I just dip the cardboard in a little bleach/h2o solution before doing whatever with it and it seems to be working out
Theres no need to sterilize the baggies since
1.) contams wont grow on the cardboard
2.) baggies are sterile out the box.
I just dip the cardboard in a little bleach/h2o solution before doing whatever with it and it seems to be working out
#46
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Posted 21 May 2006 - 10:44 PM
sterile is a strong word.2.) baggies are sterile out the box.
but thx for the additional info:bow:
#47
night_ryder
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Posted 22 May 2006 - 01:45 PM
could i not just clone by puting a piece of the inside stem from a mushroom directly into new substrate eg wild bird seed or popcorn and then let it fully colonise
#48
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Posted 22 May 2006 - 10:05 PM
yep, read all about it in the archives / vaults
