Paradox
©
Fisana

Jump to content


Photo
- - - - -

Intro and Strange Contam


  • Please log in to reply
43 replies to this topic

#21 SharkieJones

SharkieJones

    Moderator

  • OG VIP
  • 3,269 posts

Posted 02 June 2006 - 05:25 PM

Those jars don't look contamed. Hard to tell from the pics though.

#22 waylitjim

waylitjim

    A Mirror Image

  • Honorary Former Staff
  • 4,706 posts

Awards Bar:

Posted 02 June 2006 - 05:33 PM

What makes you think they're contaminated?
Common contamination colors are green and black.

#23 Hippie3

Hippie3

    DUNG DEALER

  • Founders
  • 40,642 posts

Posted 02 June 2006 - 05:34 PM

those are not contaminated
in fact
they appear to be forming pins invitro,
getting very close to 1st flush.
i'd birth them now.

#24 BitterApple

BitterApple

    Mycophiliac

  • Expired Member
  • 44 posts

Posted 02 June 2006 - 07:17 PM

What makes you think they're contaminated?


It's yellow. Or yellowish brown. And slimey looking. And while it's hard to tell in the jar, it doesnt look like any of the yellow spots are attached to the mycelium network. They appear to only be spots on the glass.

My intention was to grow invitro. Should I wait it out?

#25 Hippie3

Hippie3

    DUNG DEALER

  • Founders
  • 40,642 posts

Posted 02 June 2006 - 08:47 PM

if invitro is your intent
then yes, let them grow.
but that doesn't mean that you cannot open the lid
or even remove the cake if needed
now that it has finished colonizing.

i do see a few black spots near the verm barrier
so i'd pop open that lid,
shake off the verm
and slide the cake out,
remove that blackness
give the cake a brief dilute bleachwater dip
and then return it to a clean jar.

#26 BitterApple

BitterApple

    Mycophiliac

  • Expired Member
  • 44 posts

Posted 02 June 2006 - 08:54 PM

Will do. Thanks Hippie

One question: I can tell from the other jar I opened two days ago, that I will not be able to "shake off" the verm. Mycelium colonized much of it (more than 70%), all the way through the top. I had to cut it off with a knife in that jar.

Should I still cut it off of these cakes, or should I let the verm stay intact? Thanks again for the help - very much appreciated.

#27 Hippie3

Hippie3

    DUNG DEALER

  • Founders
  • 40,642 posts

Posted 02 June 2006 - 08:57 PM

if it's attached and not black
leave it
just shake off the loose

#28 BitterApple

BitterApple

    Mycophiliac

  • Expired Member
  • 44 posts

Posted 03 June 2006 - 11:06 AM

It's done.

During the process I swabbed the yellow spots with a q-tip and you guys were (of course) correct, they are not contams. I dont know what I'd do without you guys. Actually I do - I'd have thrown those jars away. :eusa_naug

And now I know the second try wasnt contam'd, and the first try only stalled because of lack of air. Thanks a million! :bow:

#29 Hippie3

Hippie3

    DUNG DEALER

  • Founders
  • 40,642 posts

Posted 03 June 2006 - 11:13 AM

:love:
a pleasure, as always.
:bow:

#30 BitterApple

BitterApple

    Mycophiliac

  • Expired Member
  • 44 posts

Posted 09 June 2006 - 04:46 PM

Hi all,

It's been 6 days since I removed the verm barrier from my invitro cakes and those little yellow spots have not grown at all since then. Nor have any more formed. Seems that growth of any kind has completely halted.

The cakes seem to have dried out noticably and there is always excess condensation on the jars. Once daily I dump a small puddle of water out of each lid, and 4 or 5 drops of water fall out of the jar. Temps fluctuate between 77-86 and I am wondering if I should dunk or just continue to wait?

Light has been on around the clock since innoculation. I did a little experiment: 3 jars were left closed for 48 hours to try to initiate pinning, and then they got fanned like the rest, which is 3 times a day. But there is no difference in growth between the jars either way - all have stopped.

I'm on day 32 since innoculation. Anticipation is killing me.... :crazy1:

#31 SIMBIOSIS

SIMBIOSIS

    Mycophiliac

  • Expired Member
  • 32 posts

Posted 09 June 2006 - 05:42 PM

Dont give it up you will get there.
Be pacient ,you told one of your cakes you cutted was not collonized in midle
so may be they are finishing it.After that they might fruit .

#32 Hippie3

Hippie3

    DUNG DEALER

  • Founders
  • 40,642 posts

Posted 09 June 2006 - 06:14 PM

dunk

#33 BitterApple

BitterApple

    Mycophiliac

  • Expired Member
  • 44 posts

Posted 09 June 2006 - 08:55 PM

They are underwater as we speak.

Hippie, in a thread found at the link below, Lazlo said in his experience Golden Teacher was a poor performer invitro. Professor Fanaticus stated in his interview that GT and Hawaiians are one and the same.

Since I have Hawaiian, should I set up a terrarium or just put them back in the jars? I'd prefer the latter option if it'll work. Thanks for sharing your knowledge


http://mycotopia.net...ead.php?t=11417

#34 BitterApple

BitterApple

    Mycophiliac

  • Expired Member
  • 44 posts

Posted 13 June 2006 - 07:14 PM

Still no primordia. Still no change in the cakes. In fact, the small 6 mm blue spot that appeared on one of the cakes after dunking hasnt even gone away yet.

Should I be fanning these 4 times a day?

Do I need to move them to a fruiting chamber?

Do I just need to wait longer and if so please tell me how long to wait before I ask again. It has been 3 full days since the dunk.

Thanks. :bow:

#35 hogwild

hogwild

    Mycotopiate

  • Expired Member
  • 1,088 posts

Posted 13 June 2006 - 07:36 PM

play the waiting game my friend. Nobody knows how long it will take, you just have to wait until you see hyphal knots and then you know your a day away from pins, then 2 days from mushrooms usually. Good luck and im glad your jars turned out alright.

#36 hogwild

hogwild

    Mycotopiate

  • Expired Member
  • 1,088 posts

Posted 13 June 2006 - 07:37 PM

if your going invitro no fruiting chamber is needed, but if you birthed the cakes and they arent in a fruiting chamber then id suggest moving them there asap.

#37 encapsulated

encapsulated

    Mycotopiate

  • Expired Member
  • 493 posts

Posted 13 June 2006 - 07:50 PM

I thought for invitro you just turned the jars upside down,waited,and gave light each day? am i missing something here b/c i have 6 fully colonized Tex jars and i took them out of my room b/c the dresser gets too cold, and i have them upside down in a different drawer in a different room with light on them each day will they grow like this or do i ned to get the top of the jars(now facing down) off of a flat surface so they can breath?

#38 BuckarooBanzai

BuckarooBanzai

    The Abe Vigoda of Mycotopia

  • Moderator
  • 8,968 posts

Awards Bar:

Posted 13 June 2006 - 08:01 PM

If you remove the lids, you need to put them into a terrarium to keep humidity up. The lids staying on is what makes invitro work - it stays super humid inside the jar without you adding any.

#39 BitterApple

BitterApple

    Mycophiliac

  • Expired Member
  • 44 posts

Posted 13 June 2006 - 08:45 PM

I havent birthed them. After they were fully colonized I took them out to remove the vermeculite barrier, then I put them back in the jars with the lid on.

I have been opening the lid 4 times a day for air exchange. I dont fan I just open the lid and put it back on since the jars are upside down and sitting on the lid anyway so I didnt think they needed to be fanned.

Am I making a mistake by opening them 4 times a day?

It has been 36 days since innoculation, and 11 days since removing the verm barrier. I think something like 13 days since complete colonization.

I read in the archives that you should not give invitro more than 2 weeks to pin. Hippie said this. Also i think lazlo said in another thread that he couldnt get hawaiins to pin invitro. That's why I asked if I should put them in a fruiting chamber instead of invitro.

#40 Hippie3

Hippie3

    DUNG DEALER

  • Founders
  • 40,642 posts

Posted 14 June 2006 - 08:28 AM

you are doing fine,
just need to relax and understand that
you cannot force them to go faster,
messing around
trying this and then doing that
is not helping.
just keep giving light and keep them warm
and they will pin,
all strains do, some substrains better than others of course,
but not gonna pin any faster in a terrarium than in a jar,
a jar is actually a better environment for triggering pinning.




Like Mycotopia? Become a member today!