Intro and Strange Contam
Posted 02 June 2006 - 05:25 PM
Posted 02 June 2006 - 05:33 PM
Common contamination colors are green and black.
Posted 02 June 2006 - 05:34 PM
they appear to be forming pins invitro,
getting very close to 1st flush.
i'd birth them now.
Posted 02 June 2006 - 07:17 PM
What makes you think they're contaminated?
It's yellow. Or yellowish brown. And slimey looking. And while it's hard to tell in the jar, it doesnt look like any of the yellow spots are attached to the mycelium network. They appear to only be spots on the glass.
My intention was to grow invitro. Should I wait it out?
Posted 02 June 2006 - 08:47 PM
then yes, let them grow.
but that doesn't mean that you cannot open the lid
or even remove the cake if needed
now that it has finished colonizing.
i do see a few black spots near the verm barrier
so i'd pop open that lid,
shake off the verm
and slide the cake out,
remove that blackness
give the cake a brief dilute bleachwater dip
and then return it to a clean jar.
Posted 02 June 2006 - 08:54 PM
One question: I can tell from the other jar I opened two days ago, that I will not be able to "shake off" the verm. Mycelium colonized much of it (more than 70%), all the way through the top. I had to cut it off with a knife in that jar.
Should I still cut it off of these cakes, or should I let the verm stay intact? Thanks again for the help - very much appreciated.
Posted 02 June 2006 - 08:57 PM
just shake off the loose
Posted 03 June 2006 - 11:06 AM
During the process I swabbed the yellow spots with a q-tip and you guys were (of course) correct, they are not contams. I dont know what I'd do without you guys. Actually I do - I'd have thrown those jars away. :eusa_naug
And now I know the second try wasnt contam'd, and the first try only stalled because of lack of air. Thanks a million! :bow:
Posted 09 June 2006 - 04:46 PM
It's been 6 days since I removed the verm barrier from my invitro cakes and those little yellow spots have not grown at all since then. Nor have any more formed. Seems that growth of any kind has completely halted.
The cakes seem to have dried out noticably and there is always excess condensation on the jars. Once daily I dump a small puddle of water out of each lid, and 4 or 5 drops of water fall out of the jar. Temps fluctuate between 77-86 and I am wondering if I should dunk or just continue to wait?
Light has been on around the clock since innoculation. I did a little experiment: 3 jars were left closed for 48 hours to try to initiate pinning, and then they got fanned like the rest, which is 3 times a day. But there is no difference in growth between the jars either way - all have stopped.
I'm on day 32 since innoculation. Anticipation is killing me.... :crazy1:
Posted 09 June 2006 - 05:42 PM
Be pacient ,you told one of your cakes you cutted was not collonized in midle
so may be they are finishing it.After that they might fruit .
Posted 09 June 2006 - 08:55 PM
Hippie, in a thread found at the link below, Lazlo said in his experience Golden Teacher was a poor performer invitro. Professor Fanaticus stated in his interview that GT and Hawaiians are one and the same.
Since I have Hawaiian, should I set up a terrarium or just put them back in the jars? I'd prefer the latter option if it'll work. Thanks for sharing your knowledge
Posted 13 June 2006 - 07:14 PM
Should I be fanning these 4 times a day?
Do I need to move them to a fruiting chamber?
Do I just need to wait longer and if so please tell me how long to wait before I ask again. It has been 3 full days since the dunk.
Posted 13 June 2006 - 07:36 PM
Posted 13 June 2006 - 07:37 PM
Posted 13 June 2006 - 07:50 PM
Posted 13 June 2006 - 08:01 PM
Posted 13 June 2006 - 08:45 PM
I have been opening the lid 4 times a day for air exchange. I dont fan I just open the lid and put it back on since the jars are upside down and sitting on the lid anyway so I didnt think they needed to be fanned.
Am I making a mistake by opening them 4 times a day?
It has been 36 days since innoculation, and 11 days since removing the verm barrier. I think something like 13 days since complete colonization.
I read in the archives that you should not give invitro more than 2 weeks to pin. Hippie said this. Also i think lazlo said in another thread that he couldnt get hawaiins to pin invitro. That's why I asked if I should put them in a fruiting chamber instead of invitro.
Posted 14 June 2006 - 08:28 AM
just need to relax and understand that
you cannot force them to go faster,
trying this and then doing that
is not helping.
just keep giving light and keep them warm
and they will pin,
all strains do, some substrains better than others of course,
but not gonna pin any faster in a terrarium than in a jar,
a jar is actually a better environment for triggering pinning.