Easy Ultra-sonic spore suspension
Posted 23 April 2005 - 03:27 PM
Posted 23 April 2005 - 05:13 PM
Posted 23 April 2005 - 11:44 PM
Posted 24 April 2005 - 01:16 AM
And exactly what is fresh?
Posted 24 April 2005 - 11:14 AM
The flip side is that it is likely that spores kept in the right conditions for years if not decades, should be able to grow. Oxygen and heat are the main enemies.
Posted 24 April 2005 - 10:53 PM
Food for thought. I'd like to find a way to get consistently fast colonization. Say, under two weeks for a 1/2 pint jar. If I used LOTS of fresh spores in a syringe I wonder how fast I could turn around a harvest?
Posted 25 April 2005 - 01:51 PM
get good with PF jars and then move on to a bulk tek, casings, etc.
Posted 26 April 2005 - 01:14 PM
I am interested in live mycelial syringes but am still working on it. No success so far.
Posted 23 December 2005 - 11:03 AM
Posted 23 December 2005 - 11:37 PM
i'm sure they can be gotten cheaply at wal-mart or good will maybe....
Posted 24 December 2005 - 12:16 AM
but i'm sure if you got one of the nice metal ones used for cleaning tattoo equipment i would work much better.. from what i saw they run about 50-60
personally i don't really think they are nessasary for most of us mycos... unless you just really wanna make pro-style syringes.. or are trying to get monocaryotic cultures going threw extreme spore dilution.. which also requires a nice microscope
Posted 24 December 2005 - 06:09 AM
Posted 31 May 2006 - 03:37 AM
heres a way anyone owning a ultrasonic humidifier can use it to suspend spores...
First take the top off and put two bottle screw caps back to back and fill
the top one with water. Now put this under the water sensor line. put at least a 1/4 inch of water in the entire bottom of the humidifier. for this to work, The water in the top of the bottle cap under the sensor NEEDS to be touching the water in the bottom of the humidifier.. this is done with a small piece of tin foil... looks like this..
here it is with it turned on...
now, I shoot up a vaccutainer filled with spore juice from a print. Then i
just turn the humidiifier on and stick the bottom of the vaccutainer into the
bubbling water right above the ultrasonic element... if you stick it right on it,
it won't work right, there won't be any vibrations. I'm not sure if ultrasonics
are good for you or not so i use something to hold the vaccutainer above it
don't hold it there for too long... a little bit goes a long way. the vaccutainer
starts to get warm after a bit. this works unbelievably well, yo. :)
Edited by Sidestreet, 05 October 2016 - 04:59 AM.
Posted 31 May 2006 - 04:13 AM
Posted 31 May 2006 - 07:30 AM
starts to get warm after a bit.
yep, the agitation of the water molecules
by the ultrasound
so watch the water temp if you operate it long time.
it will also generate heat inside you
so don't touch it for long.