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Easy Ultra-sonic spore suspension


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16 replies to this topic

#1 mikepua

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Posted 23 April 2005 - 03:27 PM

What is a good way to suspend spores in your syringes? I have heard of using 4 drops jet dry to 100ml's of H2O. Has anyone tried this or does anyone have a better tek?

Thanks

#2 taoistshredder

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Posted 23 April 2005 - 03:36 PM

PF's Formula:
http://archives.myco...html?1106325658

#3 insight

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Posted 23 April 2005 - 05:13 PM

I still have to get around to using Jet Dry. I've had a bottle sit around for a month now and havent used it.

#4 anticheffy

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Posted 23 April 2005 - 11:44 PM

if I make syringes they dont sit around long enough to worry about it

#5 chill

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Posted 24 April 2005 - 01:16 AM

How important is it to use 'fresh' spores? Fanaticus went on about how that is the most important thing next to cleanliness.

And exactly what is fresh?

#6 shedthemonkey

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Posted 24 April 2005 - 11:14 AM

Chill...from my few humble experiences...fresh spores (less than 3 months old) will make a very fast and healthy mycellia. The first couple of spore syringes I tried were slow colinizers until I managed to print my own and make my own syringes...you might say cuz i put a ton of spores in a syringe that higher spore density was the reason for faster and more vigorous colinization. Still don't have enough experience to say...but there is NOTHING like self sufficiency and fresh spores. :)

The flip side is that it is likely that spores kept in the right conditions for years if not decades, should be able to grow. Oxygen and heat are the main enemies.

#7 chill

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Posted 24 April 2005 - 10:53 PM

hhhmmm,

Food for thought. I'd like to find a way to get consistently fast colonization. Say, under two weeks for a 1/2 pint jar. If I used LOTS of fresh spores in a syringe I wonder how fast I could turn around a harvest?

#8 max

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Posted 25 April 2005 - 01:20 PM

Patience is a virtue.....apparently...

#9 Soliver1

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Posted 25 April 2005 - 01:51 PM

If speed is a large concern, you may try mycelial syringes, but a better way is to
get good with PF jars and then move on to a bulk tek, casings, etc.

#10 chill

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Posted 26 April 2005 - 01:14 PM

Speed isn't really an issue, neither is patience. I'm pretty comfortable with the times. It's more the spirit of discovery. If there's a better way let's find it, so to speak.

I am interested in live mycelial syringes but am still working on it. No success so far.

#11 MurCurY

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Posted 23 December 2005 - 11:03 AM

I was reading through the archives and came across something where people are using "ultra sonic" something to disperse their spores in solution. What is this and where/how much is one of these?

#12 suckerfree

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Posted 23 December 2005 - 11:37 PM

i believe that is the ultra sonic JEWELRY cleaners....
i'm sure they can be gotten cheaply at wal-mart or good will maybe....

#13 Guest_lost_onabbey_rd_*

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Posted 24 December 2005 - 12:16 AM

i bought a cheap plastic one from Fredmens jewlers... got it for $20 and it sucks
but i'm sure if you got one of the nice metal ones used for cleaning tattoo equipment i would work much better.. from what i saw they run about 50-60
personally i don't really think they are nessasary for most of us mycos... unless you just really wanna make pro-style syringes.. or are trying to get monocaryotic cultures going threw extreme spore dilution.. which also requires a nice microscope
LOST

#14 marsofold

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Posted 24 December 2005 - 06:09 AM

I have one that my work was gonna throw out when they bought a bigger one. Never tried it, though...

#15 fahtster

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Posted 31 May 2006 - 03:37 AM

heres a way anyone owning a ultrasonic humidifier can use it to suspend spores...
First take the top off and put two bottle screw caps back to back and fill
the top one with water. Now put this under the water sensor line. put at least a 1/4 inch of water in the entire bottom of the humidifier. for this to work, The water in the top of the bottle cap under the sensor NEEDS to be touching the water in the bottom of the humidifier.. this is done with a small piece of tin foil... looks like this..

ultra1.JPG

here it is with it turned on...

ultra2.JPG

now, I shoot up a vaccutainer filled with spore juice from a print. Then i
just turn the humidiifier on and stick the bottom of the vaccutainer into the
bubbling water right above the ultrasonic element... if you stick it right on it,
it won't work right, there won't be any vibrations. I'm not sure if ultrasonics
are good for you or not so i use something to hold the vaccutainer above it
like this...

ultra3.JPG

don't hold it there for too long... a little bit goes a long way. the vaccutainer
starts to get warm after a bit. this works unbelievably well, yo. :)

fahtster


Edited by Sidestreet, 05 October 2016 - 04:59 AM.


#16 hogwild

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Posted 31 May 2006 - 04:13 AM

yup i do this method for syringes also. Thanks for the input man. :smokin:

#17 Hippie3

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Posted 31 May 2006 - 07:30 AM

the vaccutainer
starts to get warm after a bit.



yep, the agitation of the water molecules
by the ultrasound
generates heat
so watch the water temp if you operate it long time.
btw
it will also generate heat inside you
so don't touch it for long.

see http://archives.myco...ow.cgi?5/175585




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