
how many times can i g2g transfer
#1
Posted 02 June 2006 - 06:45 AM
- Instasamkabit likes this
#2
Posted 02 June 2006 - 07:24 AM
#3
Posted 02 June 2006 - 07:46 AM
peace
siam
#4
Posted 02 June 2006 - 09:40 AM
#5
Posted 02 June 2006 - 10:05 AM
#6
Posted 02 June 2006 - 11:58 AM
#7
Posted 02 June 2006 - 03:42 PM
has anyone used popcorn as spawn to a bulk substrate, but instead of using the whole jar, they used the first 1/5 to spawn to another jar, so if i had 4 jars i would g2g a 1/5 to another jar and then use the other 4/5 to spawn to straw. then i could use the 4 new jars to g2g to 20 new jars while i have a casing going. then do this over and have 75 jars.
just a thought
#8
Posted 02 June 2006 - 05:36 PM
And reached a point where the strain had obviously lost some positive attributes, or fail to fruit at all.
Just interested to see if anyone actually has data firsthand.
Ive done 5 or 6 and just went back to spore so as to not push my luck but the strain still seemed fine to me
#9
Posted 02 June 2006 - 05:43 PM
fahtster
#10
Posted 02 June 2006 - 10:08 PM
Try adding nutrients to substrates then going to nutrient poor substrates. I've seen this done with cutures that had been transferred over 30 times with fruiting yields identical to earlier cultures. The same method can be applied to agar.
#11
Posted 03 June 2006 - 11:02 PM
mycelium keeps a broad range of digestive enzymes. This is very important
when maintaining good viable mycelium. Same thing when holding cultures on
agar. Bump it back and forth on PDA and MEA. I did much research on this
many years ago. We found out that the little protozoa, the "Rotifer", went
exactly 57 generations and then died when held on the same media. If it was
ever changed, from the date of the change, it went again for another 57
generations. Also, when the change on the agar is made, the mycelium will at first travel very slow. Then, it will start growing very fast. It is this section of fast growing mycelium you want to use. It will be the best........slp/fmrc
- ozb, Mycoma and Instasamkabit like this
#12
Posted 04 June 2006 - 01:02 AM
fahtster
#13
Posted 04 June 2006 - 02:04 AM
#14
Posted 04 June 2006 - 08:25 AM
#15
Posted 04 June 2006 - 09:49 AM
Has anyone here actually done it to the limit?
And reached a point where the strain had obviously lost some positive attributes, or fail to fruit at all.
Just interested to see if anyone actually has data firsthand.
Ive done 5 or 6 and just went back to spore so as to not push my luck but the strain still seemed fine to me
I just stop at 3 transfers now because of this happening to me. By then, i'm sick of the strain anyways.
#16
Posted 04 June 2006 - 10:32 AM
#17
Posted 05 June 2006 - 10:05 AM
mushroom mycelium found in Oregon covered 2,200 acres and was 2,400
years old. "August 5, 2000 Montgomery Advertiser, Page 6A" did the story
and it was placed in the #48 "THE MUSHROOM CULTURE", The Journal of
Mushroom Cultivation (TMC). You can only guess at all the differant types
of media this mycelium consumes while it is growing on 2,200 acres! No
digestive enzyme problems here......grin. slp/fmrc
#18
Posted 05 June 2006 - 11:10 PM
#19
Posted 24 January 2008 - 12:10 PM
#20
Posted 24 January 2008 - 12:40 PM
so keep starting from your master and you get very vigorous growth. so you won't ever have to go past 2 gens grain to grain if you don't need to with a good clone. intially you can do all kinds of things but keeping about 10 masters in the fridge isn't out of question. you can then slowly work your way through them and even go 3 gens if you want, but it isn't necessary. if one quart makes 10 quarts and so on. 10 quart size masters that you can draw up mycelia from to make lc syringes, or just g2g to grain should last you a very long time.