how many times can i g2g transfer
Posted 24 January 2008 - 12:57 PM
Posted 15 February 2008 - 05:54 AM
Posted 15 February 2008 - 06:27 AM
Posted 16 February 2008 - 07:33 AM
Posted 16 February 2008 - 07:54 AM
how many times can i g2g before it would be better to start a new myc colonsisation. one can only g2g so many times before the myc gets tired and old and more suceptible to contams
You can push the boundaries on that if you change up your substrate some.
Posted 16 February 2008 - 11:31 AM
The only noticeable difference in gene expression has been 80% sterile shrooms ( no spores ) at this point.
An isolate may work and perform differently than a clone or a multi spore mycellium sample during grain to grain transfers.
It would make sense that they would perform different, because they are slightly different in genetic make up.
I.e an isolate contains many genes that may express themselves in a few ways when fruited. Think of it as picking the best 7 shrooms (sets of genes) out of a flush and copying them.
A clone is a single set of genes copied. IMhO clones won't last as long transferring as isolates because the genetic structure doesn't have other genetic information to dominate when it fails ( 1 shroom vs. 7 dif shrooms)
Multispore can contain many different sub strains in one jar all constantly competing. IMO ms would last the longest # of transfers transfers.
IMO, the more genetic varity that you have the longer your transfers will last. The less genetic varity that you have, it will become more prone to errors of copying those genes.
I was going to start a new thread on this cause I thought it was interesting and this thread was in my subscription box when i logged on lol coincidence.
using a high ratio of colonized spawn to fresh grain may also help the sample transfer well for a longer period of time. ime, Less genetic copying = more genetic stability.
he plans on running the isolate until some kind of failure occurs.