I didn't find anything on alkaloid content at different growth stages yet, but I found this little nugget to confuse us more.
Enhancement of Indole Alkaloids Produced by Psilocybe cubensis (Earle) Singer (Agaricomycetideae) in Controlled Harvesting Light Conditions
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran
Bioscience Faculty, Shahid Beheshti University, Evin, Tehran, Iran
Department of Biology, Shahid Beheshti University, Evin, Tehran, Iran
Indole alkaloids of Psilocybe cubensis have been reported to be the potential candidates for drug discovery in central nervous system (CNS) disorders. In this research, the effect of the harvesting light on increasing the active alkaloids of P. cubensis was investigated. Three different lighting conditions, that is, dim daylight, indirect daylight, and darkness were used to harvest the mushrooms. A simple one-step extraction method involving the homogenization of the dried fruit bodies of fungi in chloroform was used. The psilocin content of the mushrooms was analyzed by derivatization with N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA), followed by the GC-MS technique. This investigation showed that the psilocin content of the mushrooms was highly dependent on the lighting conditions. Variations could amount to 100-fold increases in the samples harvested in the dark condition, compared to the samples harvested in the light conditions. Therefore, preventing natural light in the harvesting chamber could be considered to have a considerable effect on the content of the psychotropic component of P. cubensis and, hence, the psycho activity of the mushroom.
Edited by TurkeyRanch, 16 July 2014 - 11:09 AM.