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Communal Woodlover Grow log


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#3581 Ferather

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Posted 23 January 2019 - 02:32 PM

Azure wood spawn sample to T-Gel (updated recipe, today). See my signature for the updated recipe.

Turns out the vendor wood spawn has piggy back invaders, so I'm cleaning it up.

 

I'm using T-Gel plain, which means no optional malt extract.

 

IMG_20190123_155942.jpg IMG_20190123_160756.jpg



#3582 hyphaenation

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Posted 24 January 2019 - 02:21 PM

Has anyone tried woodlover infused honey? 

https://mycotopia.ne...-magical-honey/

It's incredibly potent and it seems to get stronger after several months in the fridge... 



#3583 Ferather

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Posted 25 January 2019 - 02:25 PM

@ hyphaenation

 

Didn't an ancient culture use honey to preserve mushrooms? I'm 100% sure I read that somewhere or was told that.

 

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Recovery and adaptation has occurred, about the right time scale, 2-3 days for the first transfer.
Sorry for the image quality, these are different containers with different lids.
 
The spare room temperature is around 20-22°C (68-71.6°F).
 
IMG_20190125_184705.jpg IMG_20190125_184849.jpg

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#3584 Seeker2be

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Posted 26 January 2019 - 02:33 PM

An observation: I have a fridge out in the garage with a lot of 3-4 year old  neglected cultures and slants.  I finally cleaned up the smelly fridge and had a few slants of azures (4 years old).  I took a chance and put them on fresh agar and they took off!  So slants keep for years is what I learned in my neglect tek unintentionaly..


Edited by Seeker2be, 26 January 2019 - 07:44 PM.

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#3585 Ferather

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Posted 27 January 2019 - 07:19 AM

Azure wood sample to T-Gel, sorry for the image quality, I will switch back to my other containers.
The infection appears to be preventing the Azure from growing well, it's very slow.
 
No visible infection on the T-Gel, one drop of metabolites on the wood.
 
IMG_20190127_105924.jpg IMG_20190127_105956.jpg


#3586 hyphaenation

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Posted 27 January 2019 - 12:17 PM

Ferather ...how's it going on fruiting ps.woodlovers ? You had said a couple years ago that they are as simple as oyster's to fruit indoors ... Did I miss the fruiting tray photos?


Edited by hyphaenation, 27 January 2019 - 12:17 PM.

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#3587 Sicshroom

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Posted 28 January 2019 - 09:05 PM

Ferather ...how's it going on fruiting ps.woodlovers ? You had said a couple years ago that they are as simple as oyster's to fruit indoors ... Did I miss the fruiting tray photos?


I would love to learn this trick as well, I have an island I'm building of p.cyan and would love to fruit it like oysters

#3588 Ferather

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Posted 29 January 2019 - 08:40 AM

@ hyphaenation

 

That project was taken over by moricz, who unfortunately stopped posting on this forum. He has succeeded multiple times with various species indoors (and got TC).

The data he put out over the last 2 years is equal to what I was saying with cold loving King oyster. Same substrates etc, cold temperatures.

 

Triggers: Cold temperatures (can vary with species), changes in temperature (cold shock), ample water and replenishment (rain).

 

In addition, dunking a cake in cold water, from room temperature, induced pinning for me with azures.

 

====

 

Azure wood spawn sample to T-Gel (plain), I'm going to start another run with my other wider containers.
Note, T-Gel needs more oxygen than normal, standard petri setups may not work well.
 
Currently pushing towards air, although it may be due to contamination.
There are now 4 drops of yellow metabolites.
 
IMG_20190129_140358.jpg

Edited by Ferather, 29 January 2019 - 09:22 AM.

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#3589 Deleena24

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Posted 29 January 2019 - 12:36 PM

If you're doing an indoor Azure grow I would love to know more. I have a couple pounds of colonized rye.

I also spawned corn to 5 pounds supplemented sawdust (95% sawdust, 5% bran) and growth was beautiful for about a a week, then the bag got completely covered in trich. THICK trich.

I once heard Azures can fight off trich, so I isolated the shit out of it, and forgot about it for maybe a month or 2.

Amazingly, the Azure mycelium completely colonized the rest of the bag. I dont know if it ate the trich, or simply outcompeted it, but I cannot use it indoors because there are tons of green spores still covering the bag and smeared on the colonized block. I'll take a picture if anyone is interested.
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#3590 Ferather

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Posted 30 January 2019 - 06:47 AM

Moricz is using the plant pot method, like the one in my journal. He has tested various methods including my own. Ideally you should read his information, more than me try to explain it.

However so far he has proven my data and what I said is correct. Moricz colonizes at room temperatures, and then fruits it at 16-18°C, which takes about 6 weeks.

 

There are members of another forum that are also using my WL-Tek recipe with Azures, and another who is using dry dog food (as enrichment).

If I remember correctly, Moricz found that some strains of certain species can tolerate up to 20°C when fruiting.

 

----

 

https://www.shroomer...=1&sort=d&way=d

https://www.shroomer...25&sort=r&way=d


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#3591 Ferather

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Posted 06 February 2019 - 11:47 AM

Export from another forum, the method also applies to gourmet lignicolous mycelium.
 
====
 
Adapted Cubensis, from dry spores, order: WL-Tek + sucrose peg > T-Gel (Recipe A) > T-Gel (Recipe B) > Brown rice.
 
IMG_20171116_170505.jpg IMG_20181022_192802.jpg IMG_20181122_133646.jpg IMG_20190115_215545.jpg IMG_20190129_125639.jpg
 
Recipe A: 100g Water, 2g Agar, 1.6g Black Tea (0.6g Soluble), 0.8g Sucrose, 0.04g MG.
Recipe B: 100g Water, 2g Agar, 3.2g Black Tea (1.2g Soluble).
 
Note: T-Gel requires more oxygen than normal, some setups may not be suitable.
 
----
 
Cubensis producing knots on Recipe A, likely due to lower carbon content (tea phenols).
 
IMG_20181222_124016.jpg IMG_20181222_124329.jpg IMG_20190101_115805.jpg IMG_20190101_120008.jpg IMG_20190101_120201.jpg
 
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Brown rice, not much of the wedge left, it turned clear and shrunk, no contamination.
 
IMG_20190202_120348.jpg IMG_20190202_120528.jpg IMG_20190205_133013.jpg IMG_20190205_133120.jpg
 
----
 
Full WL-Tek and T-Gel recipe's in my signature below.

Edited by Ferather, 06 February 2019 - 12:11 PM.

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#3592 hyphaenation

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Posted 10 February 2019 - 06:46 PM

This is not the right place for cubensis pics/updates.This is a woodlovers thread. 

Thank you. Good day.


Edited by hyphaenation, 10 February 2019 - 06:50 PM.

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#3593 Ferather

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Posted 11 February 2019 - 10:18 AM

I apologize, the method and assembly is lignicolous (wood loving). I was adapting Cubensis to decay phenols and cellulose as a lignicolous mycelium would.


Edited by Ferather, 11 February 2019 - 10:19 AM.


#3594 Sicshroom

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Posted 11 February 2019 - 03:12 PM

So I read the links and it doesn't seem like the indoor fruiting is gonna be to hard with the ps.cyans that I've been building up. The tub you see was started in September from specimens from an outdoor bed and have expanded to wood chips to build an island for planting slow growth and not having a reliable place to put an active bed around my neighborhood I will now try with some confidence to fruit from said tub. As you can see I didn't case this last night, Its been maybe 2 weeks and we can see nice white strands growing up nicely. In a few more weeks I will try to induce fruiting by exposing it to my outdoors shop but where it will get natural sunlight. The shop is insulated but not temp controled so I'm hoping to wait til March when the chances of snow are almost gone but temps are also below the 60s. I'll post as I get more to show

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#3595 Bobotrank

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Posted 20 March 2019 - 09:47 PM

Sub'd up, and very happy to have found this thread.  

 

I have currently a syringe of Cyanescens and Azurescens both.  Drained about half of them into some spawn bags a couple months back that ended up contaminating on me (they were admittedly old).  The Az took off fast, but then couldn't recover after I broke the bag up.  Have plans to try again this week after I PC some more rye.  Keep up the good work people.


Edited by Bobotrank, 20 March 2019 - 10:00 PM.


#3596 Sicshroom

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Posted 20 March 2019 - 10:02 PM

That is a loaded question. Some people are gonna tell you no need and they rye will do for spawn where others will tell you yes. All I can tell you is to try a few methods and get good with agar or be willing to spend more on syringes. Wood lovers are no joke and slow growers I've had my cyans 3 years and no fruits. I'd saw get a book write down 3 teks you can try and record your results see what grows best for you
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#3597 Bobotrank

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Posted 21 March 2019 - 02:23 AM

Thanks. Imy initial plan was syringe>rye>g2g>sawdust/hardwood chips under coardboard. I’d like to get a shit ton of spawn going and maybe try a few different approaches. Cyans grow wild in my area, as do Alenii (which I’m going to try and get my hands on). I’ve got some cool, north facing corners of my backyard that might be doable so long as I can keep them wet.
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#3598 Ferather

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Posted 21 March 2019 - 10:57 AM

It's suggested to syringe (spores) > agar > analyze the agar for any visible contamination** > agar wedge(s) to sterilized grain > continue as planned (g2g or spawn).

** If contaminated repeat the agar process by transferring a small outer sample to fresh agar, rather than spores again, post pics if help is needed.

 

Good luck.


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#3599 Bobotrank

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Posted 21 March 2019 - 01:45 PM

Now that is some way better tek than my noob tek. Thank you. I’m just diving into agar, but my first dance I’ll use the Az and Cyans again. Think 1-2cc’s per dish? Think that’s about all I have left.

Just spluffed some grain this morning with aforementioned strains.

#3600 Seeker2be

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Posted 21 March 2019 - 09:04 PM

OK went to ground zero where azures grow , Cape Disappointment, WA. I studied the terrain and the substrates in many areas..  I saw very few spots of wood chips and lots of areas of dune grass where they are said to grow in Dec.  I pulled some dune grass plants to place in an area at home and also cut leaves to see if they colonize them.  I am perplexed by the lack of wood chips there.  Any thoughts?  Ligneous  dune grass, some pine needles and pine cones,  few alders  is what I saw.


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