4492 replies to this topic

[Arathu]

It's nice when there is an intended infection......

 

IME rye berries with nothing else but a simmer cooking, fairly slowly, gentle stir in well/spring water (test just like cooking pasta, bite it and look for the light spot in the middle to go away and also watch for bursting berries)

 

Then tamp and toss dry, Needles showed me a technique with colanders (just like separating chaff from grain except as in moisture winnowing) and then salt with powder ground gypsum, toss well again and let cool, to error dry is better than to error wet, and fill a quart jar 2/3 full such that condensation does not form on the glass......loose lid and aluminum foil, sterilize in PC and after cooled knock at will......that's how A does it every time. 

 

I've learned to buy small amounts of grain and use fresh over storing, personally, but the local Agway is 2 miles away.....so there is that..... 

 

Love some new fuzz in the jars......can't wait to see.....

 

A

[rockyfungus]

I'm envious of the Agar to Wood strategy, pretty Awesome what you can accomplish in this Game we Play.  Those Rhizo's got me Like...  :ohmy:"

Honestly I'm always nervous it's just reaching around the wood for air. Till I see that wood get going I'm nervous. I've done this enough that I'm comfortable with wood substrates but grains seem to contam.

 

I think my wood is infected. May have to try this again. My alchemy tools must be broken keep trying to create more wood.

Top is softwood

Bottom is soft/hard/avb (already vaped bud, not combusted so assume still has some nutrients)
I already want to hit agar again. I need help.

Doesn't seem to care for the pot too much (green pigment causing issues, or maybe fungicide/pesticides?)

Edited by rockyfungus, 04 December 2021 - 12:22 AM.

[fahtster]

Decided to try and go as simple as possible.. I’m just throwing the colonized chips into a 2.5 gallon bag, boiling new chips for an hour, straining and then shaking them up in the bag at 1:1.. then just poking needle holes (16g) every few inches and letting ‘er roll.. so far so good. This bag will make 2 bags and then 4 bags and then 8 bags lol. Should have enough to inoculate my whole yard by the spring


Figure it only needs to be 90% and above before I add more chips and I’ll just hold the sub and flip the bag upside down if condensation builds up on the bottom to dump the water out and I can always poke more holes to adjust to try and keep the condensation down

Faht

Edited by fahtster, 04 December 2021 - 03:39 AM.

[rockyfungus]

Yes the lazy approach. I tend to sterilize the initial chips. Once the myc takes hold, boiling does seem fine.
Never thought to give FAE as I'd dry out in 10 minutes.

 

You're IMO ready for beds. The more spawn the better

Edited by rockyfungus, 04 December 2021 - 08:44 AM.

[orangutan]

Thanks everyone for sharing!  I learn so much from seeing what others are doing.

 

Has anyone ever placed a colonized wood chip on agar?  To get a clean culture?  I want to create a LC but all i have is colonized wood chips.

[rockyfungus]

Thanks everyone for sharing!  I learn so much from seeing what others are doing.

 

Has anyone ever placed a colonized wood chip on agar?  To get a clean culture?  I want to create a LC but all i have is colonized wood chips.

Yes but it won't be clean. I was still able to transfer it away as woodies are so aggressive.

Edited by rockyfungus, 04 December 2021 - 11:26 AM.

[YoshiTrainer]

Maybe take your scalpel and scrape a small pile of myc from the bark and transfer that to agar? It'll still be dirty but more manageable.

Today, I'll be adding sterile sawdust to Allenii agar jars.

[rockyfungus]

Outta likes.
So I was joking last night.

 

 

I think my wood is infected. May have to try this again. My alchemy tools must be broken keep trying to create more wood.

 

Then I went to make fuel pellets and tried a different brand. Guess my ADHD saw fuel pellets and was like good enough. Was BSing with a local buddy and he said that's softwood bro it's local.

So 2 bags turned into 4. Softwood obviously holds more water.

I used to weigh everything and had a nice journal going. Now I'm just winging it...

 

[orangutan]

Update on the agar transfers..

 

I made 6 transfers from those nasty agar dishes a few days ago and it looks like one of the colonies is pure white and even rhizomorphic.  The others looked ok at first but then developed black/brown background on the original agar wedge that was transferred.. not sure what is going on there.

 

Photo shows a purple circle where I got the latest transfer from.

 

Also noticing some mold contaminants on the edge of one of the plates.

Attached Thumbnails

• 
• 

[orangutan]

Looks like the transfers were clean. Yay! One pic is a plate transferred from the nasty plates in my previous post. The other pic is a plate that was inoculated with a tiny piece of rhizomorphic mycelium from a wood chip. The medium I’m using is MEA agar with tetracycline. Saves me a lot of headaches.

Attached Thumbnails

• 
• 

[Arathu]

Very nice...those look good orangutan....coming off chips can be troublesome at times....

 

I also use antibiotic agar for work like this....

 

A

Edited by Arathu, 14 December 2021 - 10:00 PM.

[YoshiTrainer]

A T-1 of Ps. Cyan 'Wavy Cap', spores from my new favorite uncle! :)



A look to the future, subaeruginosa and azurescens.

[rockyfungus]

Just moved my 2 little tupperwares to the final wood. It's a local soft wood fuel pellet. I don't think they will struggle. Only issue is me figuring hydration of this new wood out.

Here's a collection of gourmets they all eat wood and colonize roughly the same. Soft wood just seems slower. Latest batches have been supplemented with a gluten free flour (master's mix)

moving from top to bottom. Shiitake, King oyster, Lions mane

some Kings on soft wood fuel pellets. Up to 15 food blocks and will most likely stop at 20. Tried a few 50/50 soft and hardwood too. 
Dark is hard, light is soft.

Edited by rockyfungus, 22 December 2021 - 06:41 PM.

[Arathu]

HAPPY HOLIDAY WOODIES!

 

I wish fuzzy wedges to everyone

 

and

 

Glad Yule to ALL my brothers and sisters out there.....

 

...... ......

...~...

....~~....

.....~~~.....

 

Peace.....Love..... Light

[fahtster]

Switched back to tub with cardboard in the bottom.. bag wasn’t great for condensation.. live and learn


Faht

[Arathu]

 

Try a dry paper egg carton in the bottom of the bag and build the spawn on top......in the mean time the tub looks GOOD....

 

Rhizomy for the homies......hell yeah!

 

Looks great everyone....

 

A

 

 

Attached Thumbnails

• 

[MushLuvR]

 

One Jar is ready, and the other is Right on its Heels 

 

Cyan Rye

Edited by MushLuvR, 23 December 2021 - 01:16 PM.

[Arathu]

Let that grain consolidate fully such that when you spawn it, say when making layered pasteurized wood chip spawn, it comes out of the jar in chunks of mycelium around the grain(s).....

 

Exact same thing with the wood chip spawn, really let it consolidate well before making tub/bed.....they love marbled nutritious soil filled with woody stuff.....

 

Anything the wood is missing the grain has.....the fungus will grow thick and completely dissolve the wood chunks.....

 

 

Layers much later down the line.....

 

A

[YoshiTrainer]

Ps. Cyan 'wavy cap' making the jump



Also Ps. Cyan from a different awesome individual

[MushLuvR]

Mine are just about where I like them, NO worries and long-term project...NO Hurry.  

 

Ps Cyan

Edited by MushLuvR, 28 December 2021 - 05:50 PM.