4659 replies to this topic

[Juthro]

Ow, I can't take credit for those.  That's Alice from a bygone BNL.  God bless you Alice, and I hope you are well. I always appreciated conversing with her. She truly has a golden soul, and I hope she is doing well wherever she is at today. (and not just because she used to say nice things about my photography shares, lol)

 

https://mycotopia.ne...rabbit-holebnl/

[rockyfungus]

Well Juthro got my wood moving

[Ferather]

Looks good.

[MushLuvR]

Man, I think my wood has movement as well.  

 

 

 

 

 

Classic shit @Juthro... Made me laugh immediately.  

 

 

[MushLuvR]

And the newest member of the Woodie Family I've finally got around to play with, meet Allison Azzie.  Isn't she beautiful.  Really excited to be playing with some of these exotics, truly bucket list type of experiments.  

 

I don't care how long it takes, I'm on "their" time. 

 

Shout out to a fellow fungus friend who gave me the print.  Truly Grateful, and if you need anything, LET me know...  

 

 

Edited by MushLuvR, 10 February 2023 - 09:12 PM.

[Ferather]

The Azures on WL-Tek (added nitrogen) that is a solid block of Azure mycelium (with bits of substrate), that has been stored in the fridge with low FAE (GE, low oxygen), tried to pin, but nothing worth much.

I can tell you the temperature is correct, even the humidity, however it lacks heavy hydration (rain), oxygen levels needed for fruits, and a low nutrient casing.

 

The Mushroom People - Articles (The Science in your Casing) | My original post.

 

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Notes:

 

Azures are a primary white-rot fungi (see more in my signature below), they like Oyster fungi, benefit from additional nitrogen and calcium.

Azures can most definitely breakdown cellulose absent of phenols (lignin, tannins, so on), which are acidic.

 

Acid-free paper - Wikipedia

Edited by Ferather, 14 February 2023 - 05:36 PM.

[Ferather]

Here are some images, I was not able to take images of the knot cluster, my camera did not like it at all. I did however manage to take images of the active rhizomorphs that are climbing towards the surface area.

You can see from the images, there is active materials present (its not normal to see blue-green-black mycelium otherwise) and its connecting to bits of substrate at the surface.

 

The substrate, mycelial block, looks much the same as my worktop, white with bits in it. It can be used as spawn, expanded or have samples taken.

 

Notes, the sample was successfully rescued from its previous spawn (low nutrient), and age. Some drying out in places.

 

 

 

Mycelial cord (and rhizomorphs) - Wikipedia

Edited by Ferather, 17 February 2023 - 12:57 PM.

[MushLuvR]

Alright ya'll, just another day checkin' in on the projects... Garage Growing from the Valley.  

 

 

 

And Allison Azzie just doing her thing on Agar.  This one will be too late this year since spring is about a month away, but I'll get a sliver transferred soon... probably do a pint LC, grabbed some plastic lids awhile back and made up some grain lids, and I'll use the other lids for LC's.  I need to grab a flat of pints, just haven't got around to it.  All good, just me ramblin' on like a babblin' brook.  

 

This is a T-1 straight from the spore plate.  Nice and symmetrical... not my doing... fungus Amungus must.... have a mind of its own.  

 

Edited by MushLuvR, 18 February 2023 - 12:27 PM.

[Ferather]

Treat the Azzie like a gourmet and you will get rewarded, takes a few extra steps and a bit more learning, but its very much the same. Cant say much about using straw but here is a general guide (not too dissimilar).

Personally I make enriched cakes-blocks that I sort of bury in casing-other, with casing on the top, low nutrient. Given some practice and timing results can be quite quick, and in plenty.

 

And easy one to practice with using the above approach is Gyms, as they have been proven to yield more, and richer fruits when enriched (example).

 

[Ferather]

Azures:

 

Species type: Lignicolous

Decay type: White-rot

 

Substrates: Wood pellets, paper pellets, coir, pea sized wood chips *, [to be expanded].

Amendments: Nitrogen, Calcium Carbonate, [to be expanded].

Spawn: Common methods, including seed-grain.

 

Targets: Cellulose, plant phenols (lignin, tannins, so on), other hydrocarbons.

 

pH range: Not 100% sure, ~ 5.5-9.

 

Yield: To be established.

 

 

* The smaller the better, more surface area, and better GE compared to a big solid lump.

 

 

Interesting image 1, image 2.

 

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Edited by Ferather, 25 February 2023 - 08:00 PM.

[Arathu]

It's spring...

 

 

 

A

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[Ferather]

It seems that nitrogen, phosphorus (for production) + oxygen produce the most active materials, reading from the Wiki page I previously linked for rhizomorphs, and their role in nutrient and oxygen transport and my provided images.

I also know that a mushroom fruit body acts in some way like a lung, absorbing oxygen, possibly secreting carbon dioxide, but more importantly requires anti-oxidants, to prevent self damage.

 

According the Wiki again, the active materials are a form of primative anti-oxidants, and-or byproduct of oxidative effects (such as bruising to damage + oxygen).

White rot fungi utilize oxidation on plant phenols, and other compounds, which causes them to decay, and become absorbable as carbon.

 

Nitrogen not only plays a role in protein and enzyme production (type of protein), but also anti-oxidants (actives).

 

 

Theory: Given the oxidative nature of white-rot fungi, it would make more sense such species are more potent (produce more per gram of fruit body).

 

Baeocystin:  [C11]--[H15]--[N2]--[O4]--[P]
Norbaeocystin: [C10]--[H13]--[N2]--[O4]--[P]

Psilocybin:  [C12]--[H17]--[N2]--[O4]--[P]
Psilocin:  [C12]--[H16]--[N2]--[O]

 

Serotonin: [C10]--[H12]--[N2]--[O]

Edited by Ferather, 05 March 2023 - 10:51 AM.

[Ferather]

Needs expanding as its drying out and getting lighter (losing water, slowly), the good news is I can simply add more on top soon, and see what happens.

 

[Arathu]

Makes a "gut" tube wherever it needs to.....Woodies LOVE to make thick ropes.....

 

I notice that they will also seek out a persistent moisture source like condensation or dripping too.....

 

Feed me!

 

:-)

 

A

[Ferather]

A bit off topic, but I have done some tests in the past that resulted in the Cubensis on agar link in my signature below.

 

This is, in theory, genetics from spores and media (substrate) adaption, birthed on wood.

 

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First I picked up some spores using the fine end of 1/2 a sterilized wooden toothpick (quickly flamed over) and a foil print, using sterilized metal tweezers (being fully aseptic).

 

I placed the spores into enriched paper pellets (my normal WL-Tek recipe), but also knew cellulose is very complex (decays slowly also).

I added a few drops of sucrose water (sugars, sterilized) down the peg and the surrounding area using a syringe.

 

 

It took roughly 5-6 days to germinate and grow out, which I then transferred to a used black tea bag (no real sugars).

 

 

See my signature so see white-rot after transfer 2 using black tea extracts (as if making tea) + agar.

 

 

Some transfer 1 images (peg removed).

 

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Note, black tea bags are human grade (even the cheap ones, interestingly), although I wouldn't eat them, lol.

High in nitrogen (mostly proteins), and mostly complex carbohydrates (cellulose) + phenols.

 

Frida - Food ID: 537 (fooddata.dk) < Per 100g, approx up to 3.1% nitrogen (not sure which stage of oxidation).

Edited by Ferather, 09 March 2023 - 11:42 AM.

[MushLuvR]

Well, here we are again, FULL circle on my Outdoor Ps Cyan bed.  It's been a year since it's been in the ground from last spring (nearly to the day) and I didn't think it was going to produce anything, but we have had a very wet year here in the valley and this morning I went outside to check on it since it's been a while and wet out there from flood like rains (Monterey popped a levee).  Anyway, I gave it an overview and def still nice and wet from the rains 2 days ago or so.  Then like lightning, or seeing a ghost, it struck me like I couldn't believe what I was seeing.  I was so excited and nervous for some reason, like... I just ran in the house for my camera.  This to me, is the pinnacle of all mushrooms, and truly, I want a crack at azzies to which is another future project (clean plate ready when I am), but uhhh... YEAH speechless... this is the ONE for me.  I may have just shed a tear.  I've never been SO excited for 3 mushrooms in my whole life.  

 

 

I moved the chip for the second pic.

 

 

And a stand-alone beauty!  I hope more pop, but if not, I have clone material at the very least, and hopefully a backup print or two (I have a garage tray going, but nothing as of yet).  Who knows, just excited to see anything at this point of the GOAT in my book.  Woodies For Life!  

Edited by MushLuvR, 16 March 2023 - 03:25 PM.

[hyphaenation]

Hey man

That is kind of wild as ps cyayns generally fruit outside in the late summer/fall. I agree they do look like cyans. Do you have any pics of the whole fruits? Did they stain dark blue on handling like cyans do? 

Stalk should be brilliant white and lots of bluing 

. 

Edited by hyphaenation, 16 March 2023 - 07:15 PM.

[MushLuvR]

Here in CA it can be February or March with Cyans (Our late Winter is more like others Fall temps).  We get decent weather in the valley around this time of year.  Falls are too dry and HOT still until October, but possible to fruit after Halloween before it gets too cold if weather permits until early december (ish).  It's a macro shot, the things are tiny right now, so they look bigger than they seem.  I can see another pin popping up in the vicinity below the bigger one, I just gave em' a spritz so who knows. They could easily abort out with our wild weather and winds we've been getting.  I'll keep the bed moist and see what happens. Mycelium running all over the place, they definitely aren't a marginata.  I'll know fairly soon, the bigger one looks like a small abort I'm betting.  Time will Tell.  

Edited by MushLuvR, 17 March 2023 - 08:18 PM.

[hyphaenation]

Sounds good! 

A little goes a lonnnnngggg way with cyans. I love how later they look like innocent tiny dried up sticks... but no.

[RescueU]

I just checked on the outdoor beds I made last year/this winter, there's mycelium EVERYWHERE!!