45% Wild Bird Seed Spores of your choice
45% Coir Alcohol rubbing spray
10% Used Coffee Grounds Cling film and tin foil
Vermiculture Rubber gloves and a mask
To create substrate
1. Take Coir and boil in water, until the coir has absorbed all the water it can. Tip out any excess water, but do not squeeze water from the sodden Coir. Leave in pan to cool.
2. Put the wild bird seed into a strainer and give a good rinse.
3. Pour the WBS into the pan with the coir, then add the spent coffee grounds. Mix to a light fluff consistency.
4. Spoon mix into glass beakers leaving a 30mm from the top, making sure not to compress mix or get on the edges of the top of the beaker.
5. Cover the mix with 20mm of Vermiculture and leave a 10mm gap from the top for expansion during steaming.
6. Tear two squares of tin foil large enough to cover the top of the glass, put on piece on tight and crunch up the edges around the glass. Put the send piece on loosely so that it can be easily removed later.
7. Put full glasses into a saucepan fill with water just below the tinfoil line. Place saucepan lid on top and steam in oven for an hour and a half at 150 deg .c
8. Leave to cool overnight, done.
To create spore syringe
1. Get a small shot glass fill with 50 ml of water, put cling film over the top and nuke for 60 seconds.
2. While this is being done, sterilise (Boil or Steam) syringes, knife and equipment to be used to create spore syringe. Wait to cool.
3. Very important, use a room that has had little activity, to avoid other floating contaminate spores in the environment, no fans, no windows open etc. etc. Clean work area (kitchen Surface) Wipe down with soap and water the apply alcohol spray to surface.
4. Put a face mask on and some rubber gloves, spray alcohol on rubber gloves to decontaminate.
5. Prepare area with the nuked water, spores, syringes etc.
6. Take the lid of the water, scrape a small sized amount of spore print into water, taking care to avoid directly breathing on to it.
7. Get syringe and suck and empty the syringe to mix the spores with water, also wash the residue of the side of the shot glass, once mixed place needle caps back on, place on cling film give a light spray of alcohol to keep sterile, wrap up in the cling film, place in fridge overnight. Spore syringe complete.
To inoculate the substrate
1. Clean kitchen work area down, with alcohol spray, done gloves and mask again.
2. Shake syringes to unsettle spores
3. Take the glasses and free top layer of foil, leave loose do not remove.
4. Un-package syringes take of tip and sterilise tip of needle with a flame.
5. Lift the loose lid, and inject 2ml in per injection. Push needle as far as it will go (without going through foil) inject and draw up syringe at the same time, this is to inject spores evenly throughout the substrate. Inject each substrate 3 times in an even manner.
6. Once glass is inoculated replace lid loosely, and move on to the next.
7. When all glasses are done cover holes with micropore tape.
8. Move all the substrate into a warm (27-28 C.) room to colonise
9. Leave for around 1-2 months. Done.
To make the cake
1. Again boil half the amount of coir to colonised substrate, put a bowl with a little coffee ground’s and apply cling film, nuke in the microwave for about three minutes to sterilise the coir. Leave to cool.
2. Sterilise the working area. Put on mask and gloves, Open a jar colonised substrate, scrape of the Vermiculture into a waste bowl using a sterilised spoon. Then scrape the colonised substrate into a ceramic, clean and sterilised bowl. Empty each jar as required.
3. Take the new coir\coffee mix (leave a little for the casing.) and mix with the substrate. MIX THROURLY. Once mixed pat down mix but do not compress too much.
4. We need to case the new substrate to allow the fungi a platform to grow from, apply the saved coir\coffee mix to the top of the new cake, with a thickness of about 10-15mm.
5. Seal the new cake with a couple layers of tin foil. Poke holes in the foil with a skewer, to allow air exchange. And seal holes with micropore tape.
6. Place back it airing cupboard, at 28.C in the dark, leave for around 2-4 weeks. Done.
Fruiting the cake the fun bit.
1. We need a humid warm light place to get the cake to flush. The environment should be around 80% Humidity and around 23.C.
2. Also there need to be a clean fresh air exchange so that the cake can breathe. The diagram below is what is need to create an environment for the mushrooms to grow.
Done, now just collect those spores.
Thanks for Reading, Kronos.
Edited by Kronos, 14 December 2012 - 08:09 AM.