Posted 30 June 2013 - 08:08 PM
So, i have cloned and make a liquide culture. After clone in a second petri dishes, and made a culture with dung composted and terreau....
The casing layer, a cardboard layer from my own protocol was colonized in 7 days, 6 days after, the first pin head appeard.....
So fantatstic pinhead, very...MAssive! as you can see:
Also i work with variété of united states, menace shroom psilo cubensis, and Atsrain from 'louisiane' a french colonie, at the beginnig of the farwest....
I have a théory about the activity of this shroom maybe something weird, but maybe fantastic...
so i work, with MS at first, and clone, and make a second clone with a new rectte petri dishes, and remake a new culture... To prouve this théory of 'senescence inversée' inverted senescence...
So i work in champimagik, france and after, i post there... I think, myco is the best way to post my works. Maybe more sure...
so, enjoy this pinning, the first flush wiil be.......Monstrueux!
A new tek, cardboard layer for testing colonisation. The left for a not humidified tek, the second after humidified the vermi, so i win 3 or more days of colonisation. The cardboard is colonised about 6 or 7 days after made.
I will explain, on day how to make this at must....
Go there, count!
Posted 05 July 2013 - 06:58 AM
Posted 15 July 2013 - 08:21 AM
The third flush on a cloned mushroom beginning
Very good result generally this variety does not make a lot of flush
Posted 15 July 2013 - 08:29 AM
Beautiful job my dear each pic is better than the next!
Laissez le bon temps rouler;)
Posted 15 July 2013 - 10:04 AM
Then, j can nothing, c ' is this variety which is magic, it makes wonderful things!!!!
But thanks for your message MYco DaNY!!!!
- MycoDani likes this
Posted 15 July 2013 - 01:05 PM
Awesome pics ! Thanks for sharing ....
Posted 17 July 2013 - 08:14 PM
so i post my last (i think) flush, and a little harvest with petri dishes pict
In france, i have made a growlog. The most longer growlog of all time (because i have made some shit, with the first strain i had to grow, but to much longer for explain, so....)
I want to demonstrate the clonage is maybe more productive than a MS strain spores..... So i have made three clonage wth the first shroom of each culture, with PF and menace. and with each clone one CL and one rescue dishes....entière
With PF red, it works. Difficult, but it works, with menace, i am on beginning, but,
For me, i have to work if clone with 'trainning' (in fact with a clone who 'know' the susbtrate, like agar, honey, corn, or shit) is more potential and productive than a MS, from the same Print or CL
In fact, i have work in one direction...And no realize that the reality was other...o commentary, a will give you a secret, at the end, because, i discover that young factor is maybe the most important factor in culture, with the same strain, clone, or MS
- Cindysid likes this
Posted 19 July 2013 - 04:59 AM
I harvest (goodword???) the third flush of one other second casing today, who gives to me a 70 grammes flush. With 4 shrooms. This pf red spores culture is a specifique culture with a good clone (trained) on différents substrates....
i don(t believe, but the first casing of this clonestrain is coming over a third flush, he is beginning afourth' flush!!!!
This strain is not to productive because fruits are full of water, dense and very heavy. You can see that with my pict'.....
And maybe fruit are rebiginning to colonise themselve with a small mycélium who s eating the rest of the shroom... like that.....
If you want to have over than a third flush, you must ad some water everiday, and maybe...make clone!
I will give you a response in few month because its to late to have a conclusion.
(sorry for bad english, men, you know, the french are very ...."Chauvinist has comment of his culture and neglects the foreign languages by pride (thanks reverso)"lol)
Posted 20 July 2013 - 10:50 AM
We can see tht this mycélium is very agressiv. he colonize all what i "feel"
there, a petri dishes 4 or 5 days old
I show you a part of my work. In France.
Roughly, it is a question of' training' strain has substrats.
So, we win surement of time and of l energie when mushrooms can better take advantage afterward...
Here, c ' is a clone F1
I have to afterward, make him developper in liquid culture, redo him(!) to grow, then to clone him(!), then has new culture, to arrive has can be who knows a champion???
My work with PF is the same, and i m coming at the purpose, we shall know then if a clone is more prolific than a MS. With the same Liquid culture at beginning...
( i trained also on agar, with différents types of liquide agar)
Posted 22 July 2013 - 09:16 AM
Great result, at 130grammes!!
So, maybe this fuck***clone is one of my most productive shroom, we will see that at the fivest, certainly!
Posted 23 July 2013 - 03:46 PM
The 3è génération of clone petri dishes make by myself, i arrived to the end of my expérience, now duplicated, most duplicated, and after, Liquid culture for the final expérience, and calculate the ratio MS, and training clone..
So i give you my final works, this is represente 3 culture winned, with good cloning, and culture liquide maning.
It works,!! we will see!
Posted 23 July 2013 - 04:13 PM
Posted 02 August 2013 - 06:13 PM
An impossible trick:) at first, unless you know " entrainé, or to maintain a clone "
I owe still worked above, the principle rests on cloning tekn, a lot, to agar, and liquid culture for the final results
The future will say to us hahahahahah
I leave you on these photo, really nice
J ' have at present 40 liquid culture in two refrigerator
But cubensis, c ' is less and less my trick, thus I echange on the french mycotopia
Roughly, people pay postal charges, c ' is everything, that is 10 euro, and at least I am on that so they will push to about one instead of ' to be cold at my home freedge
I ask for a single thing, 10 euro of port, and to make the maximum of print or of foto for our french topia°)
Here is a photo of my 20 first one liquid cultures, in fact a package!
But I have to make room for the panaéolus which arrive at a gallop the end of a time, for me, the beginning of an other, for the will or rouliouk, aka champa by yours!
Posted 15 August 2013 - 09:47 PM
I want to share this work i made since 6 month on french version of mycotopia, champimagik, ou asso-spores..or CHMPMGK
Since 6 month i work with a clone from a multispores culture from PF red. I have already post some pisture in this topic since the beginnig of the aventure...
I think this is a great thing to share our work, to produce more baby growers, more efficient and more intelligent of us lol...
so there is some picture about this work, on myco
first i explain what i do.
I have selected a little piece of two mushroom in a great multispores flush of pf red. This first flush produce only 4 flush, but the only thing interessting was that this culture had a good resultat also i was no certainly sur of the qualité of the culture.
But it works,
so i decided to save them (from a good cluster mushroom) on agar and decided to trainning them few time and compare.
I isolate clone on agar, repik them, and created liquid culture, and culture. After, i made the same work. Clonning from first flush best shroom from cluster, pick them on agar, and repick them on a other recette of agar and replace them in liquid culture.
today, i m at the end, because i v got on petri dishes original mushroom clone and i have to pick them on finaly agar recipe and inoculate liquid culture for the latest test, culture finale.
Multispores print, first culture, on poper substrate, and clonage
clone trained on differentes agar recette, two at minimum.
made a liquide culture.
Made a new culture. With better substrate, only with Corn spawn, and crottin.
Clone again on agar
( at this time you can see the difference ...faster and stronger resultat)
Pick on differente recette from agar. Three at minimum. DFA, DPYa, and MYa
liquid culture with the same honey since the beginning.
And last culture
you also compare the différence between two culture, the first from MS and the latest, from trainned clone.
I m today making the final liquid culture, but i have also to make culture with the first liquide culture, the MS....
We will see the result
I am very préssé to be able to compare resultats, but I know them of advance.
If my liquid last one culture works i ' shall have atteind 3 level of training, on three liquid culture, three recipes of agar, and three culturesn with differents subs...
The whole for comparison final
And here is photo
first the 2.0 liquid culture, the yellow for the honey colour at the second sugar testing
my second freedge, between eating shroom or fish or meet
a comparaison between to jar, but the first for pF red MS..Not specificly compact, and not agressive
a agar petri dishes, with my treasur, the final trained clone. I make a liquid culture today with this mycéélium and saving mycélium too on agar petri dishes, with a new recette
the jar for liquid culture, i use silicone for using like septum... You only have to stérilised them and take down them if you want to Use them.
a secret sugar: a secret from breeders. D you know certainly breeders, a good member of champimagique, but sometime maybe difficult to understand but for me a mentor... Good guy..
and for the last test, the same work for menace multispores strain. thast the first time for her, she have to colonise this petri dishes, in fact in 10 day approxymately...very good beginning, and maybe a treasure too at the end..;
Edited by champa, 15 August 2013 - 10:06 PM.