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My first six working months on petri


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#1 champa

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Posted 30 March 2013 - 12:23 AM

jdc syzygy N2 plan de travail choix final291012.jpg


conta vbacvterienne.jpg
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jdc syzygy cyanofriscosa petri mycé monocaryo 201112.jpg
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jdc syzygy premier petri 201112.jpg
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PEU spore work petri251112.jpg
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jdc syzgy repiquage controle des petri vierge .jpg
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#2 Uncle G

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Posted 30 March 2013 - 12:49 AM

I am sure there is picture nomination in it. Pretty kewll

#3 Needles

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Posted 30 March 2013 - 01:23 AM

Frame 13 would make a great valentines day card....nice work.

#4 champa

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Posted 16 February 2014 - 08:42 AM

Hello all this guys and this fantastic community,

I am back with you for this year 2014!

Then already, I have to make room a little bit at my home and my PC, , and I believe that the best way is to save my photo is to put them on-line.

I have already made it on a French -forum, my préférate, but it  planted, (buged??) as a result, I make on two or three forum at the same time the same operation, by hoping to save some one from it, whatever it me arrives...
We never know!

 

So, i post some pics from the same thème, my work(s) on petri dishes.

I have no Glove box or Hepa Système, i only base my work on rapidity and agility. With time, it's become easily, more easily in fact, but never more eaily than a glove box works, thats why i give you advice for beginner: Create your own Glove box, there are a lot of explication on the web to do it),  (with this mato's, You always will have more and more best results, than no one. and certainly better than mine)

 

In my Own stat", i realise than i lost 1/3 to 1/2 (to do one and to do an other by manipulation) of all my petri dishes.

 

I use, The classic method, with Agar-agar, dextrose, malt, yeast, potato-cooking liquid, cats food,  sugar, and some times honey...

 

The most economic you can do, is the croquette for animal and agar mix. very good result, You always have to destroy at must the croquette, because, the little stick of croquette can hide some contamination at the end.

 

I Use paper and hamer, to break the croquettes, as is it possible, very small stick, like dust, Let them in water a moment, between 6 to 12 hours, because, with this tek, the little stick of croquette will be easier to break, and after shaking go to the Stérilisation (30 minutes at 120 degrés, with the petri dishes)

 

For me, the best way to work on agar, the best mix, is the Potatoes liquid culture cooking, and some sugar, with agar, of curse, it will be a very good petri dishes possibility to work, Very transparent. With petri dishes potatoes mix you can see all type of contamination easily. like no others...let you test

 

In fact, you only need, some yeast, dextrose, and malt extract...  and?? Agar, of curse.

 

After, you can do it easily.

 

Dont by the mixture allready in the web, you can easily do it with your pretty arms!

 

A malt dose in a good shop is between 4 to 10 dollars the 200 grammes, and sometimes cheaper (in france i buy it between 10 euro the 250 grammes). I let you find, mushroom is a time lesson to live. ..... :dry:

because, In French, we say the proverb:
"Patience and cleanliness are the first foods of the culture has the house!" :biggrin:

 

After, if you do more and more, you never have the time to become boring.

 

So, in 2012 and 2013 i ve do a lot of test, on agar.

(I also product an alternative to petri-dishes,  with little stérilisated box, disposable in traditionnal shop,  but i will post the tek in a futur day. I Called them "Bogard tek", By cuting the two words "bocal"  (conserve) and 'agar"....

 

I have very good results with this because, it s seems like a liquid culture, with septum, but can bo inoculate with a seringe, and going to the colonisation stage, this stage is not possible with classic petri dishes)

 

So, after i did the petri dishes, i stock them at 20/22, in total darkness, for several month. You can easily stock them between 2 to 4/5 month. After, if you want to conserve them, put them into freedge. with freedge, you maybe can conserve them several year, but you have to do a hermétique packaging. With Plastic film, for exemple.

 

You can, like laboratory method, place your mycélium into test tubes, for doing a best conservation methode. But i never do that. I hope one day.

 

So, i speak no words more, i late you discovers my works on petri, since 2012 to 2013 (june)..

I use a signature code, for the identification, "roul" for 2010 to 2011

"ROuLi" for 2012 to 2013

and this year i adopte the ""UNcle R".... i dont know why, but french growers call me the UNcle R. to 2013 at 2015 beginning.

 

So i post, but not In real time, i only want tpo share this past time to do shroom, with the mycotopia advice and french champimagik site, the beginning for me.

 

So,

thats all folks'

(and maybe to much!

 

PEU spore work petri251112.jpg     First, test with spores from 'SporW...."

 

A lot of différent results with them. One time it s not work on liquid culture, but good result on agar. I did liquid culture with them after taking a small piece of mycélium.

 

I test them this month... I will post the result at the end of month, i hope. This is PF Classic spores. A mythic shroom for me. Every growers have to do them!

 

1-RSCN8752.jpg

 

Contamination on agar. The twice. Cob web and other I knew; but certainly, with this aspect, bactérial contaminations. This little Form are typically batérienne contamination.

 

 

contre jour petri dpya 281012.jpg

 

If you want to do good pic with petri, you can catch them in light, behind the petri dishes, like that and other on this page..

 

 

1-1-RSCN8345.jpg    What is cut in triangle or square is second one tries(essays), contrary has a direct cloning (rather cut disorderly)

 

 

1-RSCN4305-001.jpg Very intéressant mycélium. Growing like no other, very fast and constant. beautiful to see this. very regular, and promising!   This is Pf red mycélium, for the (senescence test, after second or third tentative to grow on différents sub..)

 

 

1-RSCN9819.jpg

 

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1-RSCN9828.jpg     Very intéressantn, because, you can see primordia, on this petri dishes!

 

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1-1-DSCN3744.jpg   Clonage, with little piece of shroom on center. The second tentative will be better, because, i have lost this time..

 

1-DSCN4509.jpg

 

jdc syzygy N2311012 petri 1.jpg    a little piece of mycélium, after growing a first time on agar.

 

1-RSCN9160.jpg Several petri dishes with PF red clone. My own test, to create a very agressive strain...

1-RSCN9160.jpg

1-DSCN4135.jpg

 

 

 

1-1-RSCN6180.jpg Beautiful, it isnT...??

 

see you friends!


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#5 1967FordTitus

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Posted 16 February 2014 - 09:59 AM

Champa, it is so refreshing seeing others working on Petri dishes, it is one of the many things that attracted me to Mycotopia. I have been on other forums, but was disappointed how few people on those other forums did dish work, let alone owned, or had access to a microscope. Peace y'all, 1967.
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#6 champa

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Posted 16 February 2014 - 01:47 PM

Microcospe..... One dream....I only discovers this few time the first price is to approxymately 150 euro for a good micro....

 

One day, its become reality, this time i have only the possibility to wait.... I also want to find a micro with USb port, for shade pisctures, with all possible forum about shroomm...

 

But i have to work with pan, in first. thats the reality, i m not a specialist, but im working too....

 

Thanks for your message. 1967.


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#7 champa

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Posted 19 February 2014 - 02:59 PM

Some other pictures...

 

I use the nickname 'rouliouk" in french on Association spores, the french growers meet everybody here. You can have advice or Sometghing good about culture very intéressant.

 

Champimagik, the other name, is created Since maybe 6 to 8 max years...

 

I went to champimagik, at first there is 3 years, i have all learned here, in only six month, began by PF tek, after go more and more...

 

Alll my respect are destined to the first french growers, Who believed In CHAMPIMAGIK, or CHMPMGK

 

I hope to intéresst you to go see them.

 

Im not a perfect grower, there was many guys who worked better than me.
With the french Touch, but at the beginning, all went from myco or something else, Translated in french, like the First traduction from "gaspard des montagnes'" about PF tek...

 

At the beginnig, i stoole all i can stool in hospital, to have stérile matériel, and began my first culture at 2002/2003...

In fact, after the first sporulation, everything become dark (spores), i put all in basket, believing they were all contaminated...

 

10 years after, in 2012 i Went back and started in CHMPMGK.

 

 

 

 

 

 

So i post other picture from my work. here are signatures , différents because, i change them every 18 to 24 years, also ROuLI or UNCle, Or Roul, are always myself...

 

In fact the nick name Champa  Come from our Whim french to parfume all we can parfume.   The Nag Champa is maybe the most powerfull and magbnifique Incense of the world, maybe a "chanel number 5" for indian.

 

Nag Champa for me, are the best incense of the world, i propose to buy them, to discovers a very good product, about india.

 

The better for me is the GOLOKA, but there are plenty off parfume, discovers them, i hope to Influenced by this message, and if your intérested i can send it by post office, let me know with MP ..

 

so other pictures, guys!

 

 

DSCN1128.jpg DSCN1575.jpg RSCN2438.jpg RSCN2436.jpg RSCN5218.jpg RSCN5336.jpg DSCN6467.jpg


Edited by champa, 19 February 2014 - 03:09 PM.

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#8 champa

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Posted 19 February 2014 - 03:07 PM

other pictures and more and more....

DSCN6229.jpg DSCN6230.jpg DSCN6527.jpg DSCN6164.jpg RSCN5609.jpg RSCN7740.jpg RSCN7740.jpg DSCN7739.jpg RSCN6468.jpg RSCN6469.jpg


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#9 1967FordTitus

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Posted 19 February 2014 - 03:08 PM

Beautiful work, brother! This Association spores, what is their web address? I am very interested in seeing this, is it offered in English?

#10 champa

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Posted 19 February 2014 - 03:08 PM

THe perfect strange ring is a monocariotype spores from panaéolus goliath....



#11 champa

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Posted 19 February 2014 - 03:14 PM

Beautiful work, brother! This Association spores, what is their web address? I am very interested in seeing this, is it offered in English?

 yes i give you the link:

https://portail.associationspore.com/

 

Maybe you cannot go to this french version of mycotopia.....Maybe....

 

But this is a new version, the administrator went off there is few month, a new admin came, this is "Tomestat" a fantastic guy.

 

Flanigan, was a very good Boss, and Tom too....

 

All this documentation are in french, maybe not a good think for Horrible french adepte lol.....

 

So, i post it , but i m  not sure you can have more explications than in myco....

 

do it, and give me your sensation, 1967...if you desire by MP.... All what you want, a Amricain point of vue is a intéressant thing..

 

see you..


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#12 1967FordTitus

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Posted 19 February 2014 - 03:22 PM

Went to link, all in French, trying to navigate. Sent you PM.

#13 gremlinchode

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Posted 19 February 2014 - 04:00 PM

These pictures are very inspiring and look exciting, I am waiting on my first set of petri dishes and test tube slants in the mail.  Thanks for sharing the pic's!



#14 1967FordTitus

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Posted 19 February 2014 - 04:24 PM

These pictures are very inspiring and look exciting, I am waiting on my first set of petri dishes and test tube slants in the mail.  Thanks for sharing the pic's!


You will love taking stuff to dish, gremlinchode, from what you have been learning, you will do great at it!

#15 kaid0207

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Posted 19 February 2014 - 05:05 PM

Nice work champa !!!



#16 champa

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Posted 21 February 2014 - 06:46 AM

French furthermore!

take car, has l invasion!

( But this new young has all the beginning of a talented worker, you will see by you even anyway!)



#17 1967FordTitus

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Posted 21 February 2014 - 08:19 AM

Right on , champa, you rock, brother!

#18 champa

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Posted 02 March 2014 - 01:43 PM

other pictures, more and more, i have to clean a USb disk, so i post and wash it!

 

 

 

DSCN2585.jpg DSCN1345.jpg DSCN2207.jpg DSCN2388.jpg RSCN2233.jpg DSCN1573.jpg DSCN2060.jpg conta goldenteacher300912.jpg jdc syzygy petri mya 141012.jpg jdc syzygyN2 petri en incub1.jpg jdc syzygyN2, premier petri1-161012.jpg petri penis envy clone jdc syzygy141012.jpg petri syzygy dfa jdc syzygy141012.jpg RSCN3386.jpg DSCN9885.jpg

 






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