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some redboys on pda pix :D


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#1 fucgubarn

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Posted 23 June 2006 - 02:50 AM

looks nice :D

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#2 Hippie3

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Posted 23 June 2006 - 08:26 AM

which will you choose to fruit ?

#3 fucgubarn

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Posted 23 June 2006 - 01:45 PM

thanks :D


will fruit the redboy 75% couse have not yet,
have put 4 of those 75% pins on fresh pda and in some time will make a liquid culture,after that to oat grains
:D

#4 BuckarooBanzai

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Posted 23 June 2006 - 07:22 PM

Very nice pics! Fantastic agar work. Love those "on plate" pins!!!

#5 fucgubarn

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Posted 27 June 2006 - 01:01 AM

wonder if you can see it?
there is almost invisible mycelium growing twice as fast the the regular one,
maybe i should retransfer again to check if that isn't some disease
:D

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#6 cyberd0c

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Posted 27 June 2006 - 03:00 AM

Barn that is a very nice agar isolation with fast rhizo growth, just a newb question, what is 50% and 75% is this the spore dilution?

#7 fucgubarn

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Posted 27 June 2006 - 06:01 AM

heheh :D,
it is not the spore delution!
you should better find the threads on redboys or ask someone with better english to explain couse i'm too stoned to explain right now ,couse just this morning took my last exam
:D

#8 SharkieJones

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Posted 27 June 2006 - 08:59 AM

Nice agar work. looks like you have some nice candidates to choose from.

#9 cyberd0c

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Posted 27 June 2006 - 01:53 PM

heheh :D,
it is not the spore dilution!
you should better find the threads on redboys or ask someone with better english to explain couse i'm too stoned to explain right now ,couse just this morning took my last exam
:D


I think I know now why you are reluctant to explain to a newb what it meant. that is some history there and I'm still not thru reading the lot of it!!

#10 the_chosen_one

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Posted 27 June 2006 - 01:57 PM

(tco's a little late but grabs a chair and kicks his feet up anyways):smokin:

#11 the_chosen_one

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Posted 27 June 2006 - 02:04 PM

wonder if you can see it?
there is almost invisible mycelium growing twice as fast the the regular one,
maybe i should retransfer again to check if that isn't some disease
:D


Transfer it Barn :greenboun Last time I saw something like that, it was the PFA monokaryon isolate that I work with. It moves pretty fast on agar however it grows much slower in LC. Of course I just caught the fact that these are clones :space: Still worth checking it out tho:p

cyberd0c - what Barn is saying is that the 75% redboy is the result of Roger's strain crossing it with the PR. If I have this right, the 75% indicates the amount of reddish spores the strain releases in a spore print. I believe Roger used the snake venom protoplast fusion technique rather than a spore dilution tek.

#12 cyberd0c

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Posted 27 June 2006 - 03:52 PM

cyberd0c - what Barn is saying is that the 75% redboy is the result of Roger's strain crossing it with the PR. If I have this right, the 75% indicates the amount of reddish spores the strain releases in a spore print. I believe Roger used the snake venom protoplast fusion technique rather than a spore dilution tek.

TCO thank you very much. I read most of the threads that had redboys in it but did not find the original RR thread. maybe it was lost or I'm not looking in the right place. appreciate your explanation though.

#13 Hippie3

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Posted 27 July 2006 - 06:27 PM

If I have this right, the 75% indicates the amount of reddish spores the strain releases in a spore print.


i don't think so,
i believe the percentage refers to the
genetics,
the original redboy/puerto rican cross was 50/50
then rodger back-crossed to increase the % to 75.
further back-crosses would yield increasing 'pure' redboy genetics
and breed out the strain used to revitalize the redboy [pr]

#14 the_chosen_one

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Posted 27 July 2006 - 07:06 PM

Ah, that makes more sense to me...how the hell could ya gauge the number of spores anyway :D

#15 the_chosen_one

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Posted 12 May 2008 - 03:33 PM

where's 'barn been anyways?

:love:

#16 Hippie3

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Posted 12 May 2008 - 06:01 PM

was here about 3 weeks ago




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