26 replies to this topic

[fahtster]

ok here is a tek i've been using as of lately.. it's basically using the faht jars for cloning like Golly has been... but instead of a glovebox, you are using an oven bag. i can't take pics at the moment so i just drew a diagram...
1.) put a 'faht jar' in an oven bag and zip tie leaving a good portion of the rest of the bag for the 'quarantine' section. I forgot to put it in the diagram, but you need some kind of air escape in the part of the bag that the jar is in or the bag will pop in the pc. i use 5/8 in. tubing stuffed with polyfil thats zip tied to the bag. I actually put one in each corner of the bag just in case. now pc this at 15 psi for 35 min.
2.) once cooled.. take out of pc and get your cloning tissue ready. Take an inner piece of tissue and sterilize in H2O2 3%with a sterile tweezers until it stops 'fizzing'. now take the tissue and put it in the top part of the oven bag right on top of the zip tie. Pour a generous amount of H2O2 3% over the tissue in the top of the bag. then squeeze out any extra air and zip tie that off. massage the tissue around in the 'quarantine' section for a minute or two.
3.) Now unscrew the jar lid (i'm using lids from this thread... http://mycotopia.net...ead.php?t=11247 ) using your hands thru the bag.. DO NOT OPEN THE BAG AT ANY TIME. the bag is easy to manuever from the outside.
4.) now CAREFULLY (so you don't cut the oven bag) cut the first zip tie so that the H2O2 and tissue 'flow' into the main part of the bag that the jar is in.
5.) while the bag is still closed, use your hands to maneuver the tissue into the jar (it's actually quite easy). Now screw the lid back on and tighten. again, while it's still in the bag.
6.) NOW you can remove the jar... shake the hell out it and it will be colonized in 4 days. then i just break it up, inject with water, shake it around, and suck up the myc. water to be used as innoc. :) heres the diagram....
http://mycotopia.net...=1&d=1151017432
fahtster

[Hippie3]

ever thought about a career in the graphic arts ?
:bow:

[OZ]

i think i saw this in the old archives before, but instead of a jar, there was jsut a bag of grains

[fahtster]

I wouldn't doubt it oz, it's a rockin ass tek, yo. four out of four are going good. :)

fahtster

[tbonus]

GOOD JOB FAHTSTER:bow:

[BuckarooBanzai]

Very nice. Simple and elegant.

But can you still do a turkey with the bag, that's what I want to know...

Seriously, very cool man. Excellent applications to the home mycology crowd!

[golly]

Cool faht...How easy is it to manuver the tissue into the 1/2pint...?

[stonesofthegodz]

awesome tek! how do you keep the bag from hitting the side of the PC and melting?

[fahtster]

golly... super easy... just lift the bag part up to the rim of the jar and plop it in there. :)

stones- lay out a piece of foil about two feet long. put the bag and jar in the center of one side.... then just fold over the foil and bunch it up with the bottom foil and you have a nice little pouch... just put a couple 1/2 pints upside down to keep it off the bottom of the pc where the water is. :)

thanks everyone. :) haven't thought about that hip. :) thinkin i should huh?. :)

fahtster

[night_ryder]

i can get either 3% hydrogen or 9% hydrogen will the concentration of h202 effect the myc(kill it?), will the exposher to h202 effect the myc(kill it?)

great work fahtster, will try this when i can.

[fahtster]

i think you'll be ok using the 9%... probably even better. :) just may take a bit longer for the myc. to start growing again.

fahtster

[Hippie3]

9% is far too strong,
even the 3% can be diluted with water
recommended 20:1 water:3% h2o2

[fahtster]

this is the first time i attempted cloning from a specimen. so far, four out of four are good. i used a clone from this thread to re-innoc. the jars in this thread... http://mycotopia.net...ead.php?t=11781 so the updates should prove to be very interesting. :)

fahtster

[fahtster]

ah... thanks for the chime in, hip. :)

fahtster

[fahtster]

although... i haven't used diluted H2O2 with this yet so i can't recommend diluting the 3%... i haven't had any problems with the full strength 3% killing the cloning tissue or anything.. so i would say not to dilute the 3% in this case. I don't know how the outcome would be. i was worried about the 3% actually not being strong enough cuz golly was using 35% with a rinse. but it seems to be just fine. :)

fahtster

[troutlips]

Archive material...to Cloning. :greenboun

[fahtster]

thanks trout. :) I'd also like to thank Golly for his work and the idea! :)

fahtster

[night_ryder]

how long should the clone tissue be exposed to h202 3%(diluted or not diluted)?