Faht's oven bag clone tek.
Posted 22 June 2006 - 06:04 PM
1.) put a 'faht jar' in an oven bag and zip tie leaving a good portion of the rest of the bag for the 'quarantine' section. I forgot to put it in the diagram, but you need some kind of air escape in the part of the bag that the jar is in or the bag will pop in the pc. i use 5/8 in. tubing stuffed with polyfil thats zip tied to the bag. I actually put one in each corner of the bag just in case. now pc this at 15 psi for 35 min.
2.) once cooled.. take out of pc and get your cloning tissue ready. Take an inner piece of tissue and sterilize in H2O2 3%with a sterile tweezers until it stops 'fizzing'. now take the tissue and put it in the top part of the oven bag right on top of the zip tie. Pour a generous amount of H2O2 3% over the tissue in the top of the bag. then squeeze out any extra air and zip tie that off. massage the tissue around in the 'quarantine' section for a minute or two.
3.) Now unscrew the jar lid (i'm using lids from this thread... http://mycotopia.net...ead.php?t=11247 ) using your hands thru the bag.. DO NOT OPEN THE BAG AT ANY TIME. the bag is easy to manuever from the outside.
4.) now CAREFULLY (so you don't cut the oven bag) cut the first zip tie so that the H2O2 and tissue 'flow' into the main part of the bag that the jar is in.
5.) while the bag is still closed, use your hands to maneuver the tissue into the jar (it's actually quite easy). Now screw the lid back on and tighten. again, while it's still in the bag.
6.) NOW you can remove the jar... shake the hell out it and it will be colonized in 4 days. then i just break it up, inject with water, shake it around, and suck up the myc. water to be used as innoc. :) heres the diagram....
Posted 22 June 2006 - 06:36 PM
Posted 22 June 2006 - 06:43 PM
Posted 22 June 2006 - 06:53 PM
Posted 22 June 2006 - 07:02 PM
Posted 22 June 2006 - 07:10 PM
Posted 22 June 2006 - 07:23 PM
But can you still do a turkey with the bag, that's what I want to know...
Seriously, very cool man. Excellent applications to the home mycology crowd!
Posted 22 June 2006 - 07:31 PM
Posted 22 June 2006 - 07:53 PM
Posted 22 June 2006 - 08:02 PM
Posted 22 June 2006 - 08:22 PM
stones- lay out a piece of foil about two feet long. put the bag and jar in the center of one side.... then just fold over the foil and bunch it up with the bottom foil and you have a nice little pouch... just put a couple 1/2 pints upside down to keep it off the bottom of the pc where the water is. :)
thanks everyone. :) haven't thought about that hip. :) thinkin i should huh?. :)
Posted 23 June 2006 - 07:11 AM
great work fahtster, will try this when i can.
Posted 23 June 2006 - 08:55 AM
Posted 23 June 2006 - 08:59 AM
even the 3% can be diluted with water
recommended 20:1 water:3% h2o2
Posted 23 June 2006 - 09:01 AM
Posted 23 June 2006 - 09:02 AM
Posted 23 June 2006 - 09:24 AM
Posted 23 June 2006 - 09:55 AM
Posted 23 June 2006 - 10:28 AM
Posted 23 June 2006 - 09:01 PM