ive always wanted to grab some of that myc and put it on a plate, excellent thread hyph.
as to whether it will make a difference or not- it is still multispore fwiw. you would need to
isolate the divergent sectors on agar to really get a good iso. i would recommend waiting until
you see the first knots/pins and isolate one of those from the tub wall, that way you know
you have a fruiting substrain. some substrains will be rhizomorphic, but still fruit like shit.
(voice of experience on that one)
either way, best of vibes to ya buddy and subscribed!
Exactly what I was thinking. If from multispore, and you get little to no fruits or pins or knots from the wall myc, then would it be worth isolating? And even if you did get knotting on the wall, they most likely won't reach their full potential, and there in lies a chance of poor fruit quality, i.e. size, potency, thickness. Just something to consider I suppose.
Is there a difference from isolating myc, and cloning tissue from a prime fruiting body to agar? I know the procedure differs slightly, but do the end results drastically vary?
A couple other things to consider, the higher risk of contams. And could you have achieved the same end results starting MS on agar in the first place? And possibly in a shorter amount of time.
Sounds like a great idea, was just curious as to how efficient a method it would be.
You gave me an idea from your redboy thread listed above, and sorry if its been mentioned before, but, wouldn't you get better, and quicker results cloning a prime fruiting body onto one of your milking jars, and expanding that? Basically the same thing you wanna do here, but with a proven sub strain. If I'm off on this, let me know. That's why I asked whether the end goal between isolating myc, and cloning a fruit was different.
Edited by fungi2bwith, 08 July 2013 - 10:36 PM.
Gave it more thought