Paradox
©
Fisana

Jump to content


Photo
* * * * * 1 votes

PE6 - Print -> Agar -> Transfer -> Grain -> Monotub


  • Please log in to reply
10 replies to this topic

#1 mycomattie

mycomattie

    Mycophiliac

  • Expired Member
  • 171 posts

Posted 04 November 2013 - 11:59 AM

It's been a while Mycotopia - I've been pretty inactive most of the summer.  I had a very promising Gymnopilus lute and Panaelous cyanescens (Hawaii) grows going on, but due to severe neglect and half assed pasteurization, they both succumbed to trich; which bummed me the fuck out even more.  Now that things have settled down, I’m trying to get back into the swing of things.

Spore print -> agar -> transfer -> grain -> monotub.  This is also my first time using a "traditional" monotub.

 

 

778747251-IMG106.jpg

 

 

I purchased MEA from Fungi Perfecti and plastic petri dishes/disposable scalpels from Amazon.  I followed the directions on agar jar, let cool, and inoculated the plates in a SAB. 

Honestly, my first attempts at agar are mediocre and awkward; the SAB really limits your dexterity.  I transferred some of the most rhizomorphic sectors (for practice).

 

 

778747313-IMG108.jpg

 

After three transfers, I felt I had some decent agar plates to work with (rhizo and contam free); I prepared quart jars of rye berries – pressure cooked for 90 minutes at 15psi and let cool overnight. 

I’ve G2G’s before, but this would be my first time transferring agar wedges to grain.  Again, it was awkward and clumsy working in the SAB, at least for me. 

Shook the agar wedges and grain and let them sit, on a shelf, at room temp; and sit they did – probably 6 weeks past 100%. 

I finally spawned them to bulk on August 15th.  Soaked the grains prior to spawning.

I spawned 5 quarts to ~ 7lbs of a manure based mix.  I also used a black, heavy duty, trash bag liner.

This was my first time growing using a traditional monotub – set and forget style.  Call it beginners luck, but I definitely like them – no ultrasonic humidifier to refill, etc. 

 

 

778747343-IMG109.jpg 778747370-IMG110.jpg

 

 

The bulk sub was ~ 100% colonized by the 22nd – only 7 days after spawning!  On the 22nd I initiated fruiting.

This is August 29th – 7 days after tub was put into fruiting conditions.

 

 

778747497-IMG115.jpg 778879236-IMG_2864.jpg

 

 

8/30/13

Not seeing any resemblance to PE

 

 

788579964-IMG117.jpg 788579998-IMG118.jpg

 

 

First flush....

 

 

800881035-pic3.jpg 800881021-pic2.jpg 800881008-pic1.jpg 800880945-IMG124.jpg 800880976-IMG125.jpg 800880912-IMG123.jpg

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


 



 


  • Hash_Man, MrGumball, psycheholic and 1 other like this

#2 HailHypnoToad

HailHypnoToad

    Mycotopiate

  • Expired Member
  • 369 posts

Posted 04 November 2013 - 01:38 PM

I just finished a PE6 grow as well and saw nothing  even closely resembling that species...



#3 mycomattie

mycomattie

    Mycophiliac

  • Expired Member
  • 171 posts

Posted 04 November 2013 - 03:30 PM

I just finished a PE6 grow as well and saw nothing  even closely resembling that species...

 

Yup, they just look like tall, lanky cubes...



#4 HailHypnoToad

HailHypnoToad

    Mycotopiate

  • Expired Member
  • 369 posts

Posted 04 November 2013 - 04:12 PM

Have you studied them and noticed a difference in potency?



#5 mycomaniacsirk

mycomaniacsirk

    Mycophiliac

  • Expired Member
  • 425 posts

Donator

Posted 04 November 2013 - 04:23 PM

very nice tubs!
  • mycomattie likes this

#6 Hash_Man

Hash_Man

    Old Codger

  • OG VIP
  • 2,572 posts

Awards Bar:

Posted 04 November 2013 - 05:14 PM

th_AAA00-1383584194_thumb.jpg

I just finished a PE6 grow as well and saw nothing  even closely resembling that species...

From what I've seen they look like PE6 to me, I think theres some variation in that strain and you essentaly have a isolate there ...but even if they aren't you gained a world experience in pretty advanced techniques good f*^+ng job IMHO   


Edited by Hash_Man, 04 November 2013 - 05:40 PM.

  • mycomattie likes this

#7 fabrique

fabrique

    Storyteller

  • Expired Member
  • 852 posts

Donator

Posted 04 November 2013 - 05:28 PM

this was my firends pe6 grow, sometimes it gives 6-7inch long dicks, usually in the later flushes

post-16082-138195550614.jpg


Edited by fabrique, 04 November 2013 - 05:28 PM.

  • Hash_Man and mycomattie like this

#8 fabrique

fabrique

    Storyteller

  • Expired Member
  • 852 posts

Donator

Posted 04 November 2013 - 05:33 PM

potency wise, they are more potent then the regular, 0.7g/person had a nice effect



#9 psycheholic

psycheholic

    S-W-I-M Team

  • OG VIP
  • 2,726 posts

Awards Bar:

Posted 04 November 2013 - 06:57 PM

My only PE6 attempt contammed, but every pic I see they look like regular cubes. The potency is definitely there though. I ran across some a while back and enjoyed thoroughly. I really need to get my APE, PE uncut, and PE 6 cleaned up on agar to enjoy these other varieties.

#10 fabrique

fabrique

    Storyteller

  • Expired Member
  • 852 posts

Donator

Posted 04 November 2013 - 07:24 PM

depends on the genetics really...  :rolleyes:



#11 mycomattie

mycomattie

    Mycophiliac

  • Expired Member
  • 171 posts

Posted 04 November 2013 - 07:32 PM

Thanks everyone -

 

I haven't tried any yet personally, so I'm unsure of the potency.  The PE I had grown just prior were supposedly very strong - 1.5g was almost too strong, even for experienced trippers.  After many cubes, Ps. tampanensis/galindoi ATL#7 - I'm definitely germinating some other species I haven't tried yet on agar.






Like Mycotopia? Become a member today!