While conducting g2g i spilled some grain and of course some went on the floor. I decided this would be perfect oppurtunity to set myself up for some practice at isolating away from a contamination.
What i did.....
Opened to agar plates in front of flow hood and picked up some colonized grain off the floor that has been stepped on and had lint stuck to it. I placed a single piece in each plate and placed into incubation.
After doing this i said to my self, "self, WTF were you thinking" and placed into incubation. Over the next several days i watched as the bacterial contamination showed up around the grain. On the 7th day i noticed some mycelium poking out under the grain so i tilted the jar to roll the grain to the edge and the above picture is what i captured.
What im wanting to observe is just exactly what this mycelium does. Im certain its going to retreat but if it consumes the bacteria then i would think that as long as its a good fruiter then maybe i should isolate it yes? When i first started agar work a plate like this always ended up in the decon bucket and never produced any viable myc. I would love to have a microscope to possibly identify this bacteria! I was scammed, well not scammed because i received my money back, but i found one that was listed for 1299 on clearance for 749 and waited almost six months to receive im sorry were unable to fullfill your order! Very disappointing! !!
Edited by coorsmikey, 26 May 2016 - 06:36 PM.