so in refference to my time frame question.
ive not seen growth on the spore to agar plates. But its only been 8 days.
when do i call it bust? 20 days? I wonder what the %of bunk transfers is...i mean i did 20 so somthing gotta grow...
ive got growth on a couple that were newer spores but i cant tell if its contam or fungus
id deff say that ill make a solution first next time i do plates...this spore to plate is too long. Ive got patience and itll prob be easier to wait next time but waiting 15 days to see growth is a bummer...not to mention im handleing them everyday. Im bound to fk em up befor too long i keep touching them. I need another incubator cause my spawn jars and bags are all, in the same tote and its gotten crouded..15 lb wbs bags 20 jars and 20 plates is recipe for disaster..
anyways off topic...i can shoot pics if u think u can id
There are so many variables that determine these time frames. How old were the spores is this unknown? I have had spores take 3-4 weeks to germ. So when to call it a bust? Thats subjective and would say more then 2 weeks tops for me. Not that means the plate is bad but the process isnt quick enough for me. Spore to agar probably gives the best chance at germinating a clean culture and that is the only reason i see doing it IMO. There is no clean print, i dont care how careful you are as the fruiting process is not sterile and making spore syringes or MS solutions you are playing Russian Roulette. Lets say you put an entire spore print into a 60 cc syringe, you dispense the solution a single drop at a time, you will dispense a contamination because i gaurantee you they are in every syringe and every solution. Its the concentration of the contamination that makes either viable or not. Im off track also and i do that alot so you and i are going to het along great!
I would give your playe at least 2 more weeks. If there is no signs of contaminations, which they are very easy to spot on agar, then i would think that the spore isnt hydrating. When inoculating a plate with dry spore then i would lower the agar ratio which helps the agar to remain a little more moist on the surface helping with hydration. If you used a premix then you.would not be able to do this without throwing off your nutrient ratios. Incubation is important for germination in my opinion. After that you could leave in room temperature.
Can you tell me about your agar? Type, ratio, and etc?
Inoculation method such as loop, scalpel, in a SAB or in front of a flow hood. And for sure get some pics as that always helps.