Paradox
©
Fisana

Jump to content


Photo
- - - - -

Using metabolites to fight trich and contams


  • Please log in to reply
42 replies to this topic

#1 blackdustt

blackdustt

    Mycophage

  • Expired Member
  • 180 posts

Posted 17 December 2014 - 08:14 PM

Just checked on all the monos. Had one that had a small spot of trich in the middle. I would normally just pitch the mono but I thought I could entertain myself. This mono does not have a liner and I can see all sides and bottom of the sub (just not the inside) and could only see the trich in a dime sized spot in the middle. So, to entertain myself, I preformed sub surgery but cutting out the spot and 2 inches of surrounding sub. I then took some of my freshly harvest metabolites and soak the impacted sub. I then went on to add droplets of metabolites of the entire surface of the sub to see what will happen. Also, this tub was spawned almost a week ago and seems to be 80% colonized.

 

 

---

Move this to the Mad scientist forum. Wasn't able to post in their.


  • roscoe, Cue, catattack and 2 others like this

#2 coorsmikey

coorsmikey

    (~);}

  • App Administrator
  • 2,789 posts

Donator


Awards Bar:

Posted 17 December 2014 - 08:27 PM

Calvin_Peeing.jpg
...........Trich..........

Edited by TurkeyRanch, 17 December 2014 - 11:18 PM.
Code fix

  • Juthro, catattack and Headinthehills like this

#3 TurkeyRanch

TurkeyRanch

    Dancin' Deviant

  • Honorary Former Staff
  • 5,930 posts

Awards Bar:

Posted 17 December 2014 - 09:11 PM

Cool idea.

Currently people can't start threads in the Mad Scientists forum, that will change in the next few days. Moving this there.

#4 roscoe

roscoe

    Not a mycologist.

  • OG VIP
  • 2,826 posts

Awards Bar:

Posted 17 December 2014 - 10:30 PM

You are in good company, both Paul Stamets and I (hehe) think that metabolites (myco piss if you will) are not only full of digestive enzymes but also acts as a chemical defence strategy for the fungi. 

 

How else would it convey its anti-fungal, anti-bacterial, and ant-viral compounds out into the world if not by myco piss?

 


  • MycoDani, TurkeyRanch, greenosophy and 4 others like this

#5 TurkeyRanch

TurkeyRanch

    Dancin' Deviant

  • Honorary Former Staff
  • 5,930 posts

Awards Bar:

Posted 17 December 2014 - 11:19 PM

It makes an amazing foliar spray for plants too. . .
  • zakna likes this

#6 roscoe

roscoe

    Not a mycologist.

  • OG VIP
  • 2,826 posts

Awards Bar:

Posted 18 December 2014 - 05:05 AM

I bet.


  • TurkeyRanch likes this

#7 torn2bits

torn2bits

    Mycotopiate

  • Expired Member
  • 871 posts

Posted 20 December 2014 - 02:39 PM

So if I'm getting this right in my "head" the metabolites would be a GREAT nutrient additive?
?Is That the idea???

#8 TurkeyRanch

TurkeyRanch

    Dancin' Deviant

  • Honorary Former Staff
  • 5,930 posts

Awards Bar:

Posted 20 December 2014 - 03:33 PM

Well, maybe not as a nutrient additive per say. It is waste product that the mushrooms are done with, so it will not "feed" your fungi. It will however feed a plant, either via stomata (foliar feeding) or via root drench.

I think it is a great idea to use it on contams like mentioned above, I bet it would inhibit some types. Certainly worth looking into!

Edited by TurkeyRanch, 20 December 2014 - 03:34 PM.

  • roscoe likes this

#9 blackdustt

blackdustt

    Mycophage

  • Expired Member
  • 180 posts

Posted 20 December 2014 - 04:21 PM

Just cause I love you guys.

From the GGMM book

27. Production of Primary and Secondary
Metabolites A strain's ability to compete may
be directly related to the production of primary
and secondary metabolites. All fungi produce
extracellular enzymes that break down food
sources. Myriads of metabolic by-products are
also generated. These extracellular compounds
are released through the cell walls of the mycehum,
enabling the digestion of potential food
sources. Enzymes, such as higninase which
breaks down the structural component in wood,
are extremely effective in reducing complex
carbon chains, including carbohydrates and hydrocarbons.
Secondary metabolites usually occur well after
colonization. A good example is the yellow
fluid, the exudate, frequently seen collecting at the
bottom of aged spawn containers. Pleurotus spp.,
Stropharia rugoso-annulata, and Ganoderma
lucidum are abundant producers of secondary metabolites,
especially complex acids and ethylene-related products.
These metabolites forestall
competition from other fungi and bacteria.


Edited by TurkeyRanch, 20 December 2014 - 07:27 PM.
Fixing quote

  • invisibilitysyndrome and Earthling like this

#10 MycoDani

MycoDani

    Myco Chick

  • Site Administrator
  • 9,857 posts

Donator


Awards Bar:

Posted 20 December 2014 - 07:12 PM

TR is so right on that one actually. When my mom grew she um did that.

Hey whatever the babies need.;) back to your scheduled program.

#11 TurkeyRanch

TurkeyRanch

    Dancin' Deviant

  • Honorary Former Staff
  • 5,930 posts

Awards Bar:

Posted 20 December 2014 - 07:29 PM

If I read that correctly, using it on contaminated areas or uncolonized substrate would be a viable idea. Like I said, it won't feed the growth, but maybe keep other stuff off it while it colonizes.
  • roscoe likes this

#12 blackdustt

blackdustt

    Mycophage

  • Expired Member
  • 180 posts

Posted 20 December 2014 - 09:06 PM

Have you guys read this in his GGMM book? He also has a lot of good info on "pure cultures" and "natural cultures" in his "Mycelium Running" book. Paul seems so smart. He even advises mushroom growers to test several different spawn mediums to test a mushroom culture on and then test that spawn medium to several different substrate material. He claims that the combination of spawn medium and substrate medium is a very important part of growing mushrooms. He claims that the speed by which the mycelium grows is the number 1 factor to look for when picking a spawn and substrate medium.

 

 

 

With Shiitake,
Enokitake, Maitake, and Reishi, I acquaint the
mushroom mycelium with the host substrate by
introducing to the media a 1-2 gram sample of
the sawdust directly into the liquid fermentation
vessels. This liquid inoculum is then used to
generate grain spawn. I am convinced that this
method empowers the mushroom mycelium.


#13 catattack

catattack

    Mycotopia's beast coast day shift helper. [^._.^]ノ彡

  • OG VIP
  • 6,596 posts

Awards Bar:

Posted 27 December 2014 - 05:45 PM

Just "harvested" some metabolites (myc pee) off of a colonized pan grow with a syringe. The plan is to grab some trich, throw it on agar, let it colonize, then hit it with the myc pee.. I can post it here or start a new thread (up to you balckdustt)

 

love,

catattack 

Attached Thumbnails

  • PC2704221.JPG
  • PC2704211.JPG
  • PC2704201.JPG


#14 blackdustt

blackdustt

    Mycophage

  • Expired Member
  • 180 posts

Posted 27 December 2014 - 06:23 PM

Nice. I'm just waiting on a PC to run some agar experiments like what you are doing. I have heard of reports that it is effective for bacteria. Please, post here so I can see what will happen. I'm very interested.



#15 catattack

catattack

    Mycotopia's beast coast day shift helper. [^._.^]ノ彡

  • OG VIP
  • 6,596 posts

Awards Bar:

Posted 27 December 2014 - 06:32 PM

got it, just noc'd up some agar with trich (lord knows that I've never had a problem growing trich)


Edited by catattack, 27 December 2014 - 06:37 PM.

  • Cue likes this

#16 zakna

zakna

    Mycotopiate

  • Banned Member
  • 407 posts

Posted 27 December 2014 - 10:04 PM

I'm closely following this I'm doing like experiments
  • catattack likes this

#17 happy4nic8r

happy4nic8r

    cyans rule!!

  • OG VIP
  • 3,426 posts

Donator


Awards Bar:

Posted 18 February 2015 - 05:50 PM

got it, just noc'd up some agar with trich (lord knows that I've never had a problem growing trich)

Did this ever produce anything, or did you find the missing link to all mycology and just don't want to share until it's patented.???


  • coorsmikey and catattack like this

#18 coorsmikey

coorsmikey

    (~);}

  • App Administrator
  • 2,789 posts

Donator


Awards Bar:

Posted 18 February 2015 - 06:30 PM

Edit: oops

Edited by coorsmikey, 18 February 2015 - 06:32 PM.


#19 hyphaenation

hyphaenation

    Former Staff

  • Honorary Former Staff
  • 13,130 posts

Donator


Awards Bar:

Posted 18 February 2015 - 06:33 PM

I remember reading an interesting study on using a lactate (sp) extraction of a secretion of trichoderma that was inert but fooled the mycelial colony into beefing up its defenses because it thinks active trich was present. This helped stave of the real trich... interesting stuff.
  • catattack, zakna and happy4nic8r like this

#20 zakna

zakna

    Mycotopiate

  • Banned Member
  • 407 posts

Posted 18 February 2015 - 06:36 PM

And they called me crazy thinking of trying to give myc a vaccine...
  • bright likes this




Like Mycotopia? Become a member today!