Using metabolites to fight trich and contams
Posted 18 February 2015 - 06:58 PM
I am continually reading really interesting stuff about the fungi world, almost to where I can't tell which is believable.
So like the substance itself, a real trickster, that presents reality as if it were truth, and vice versa!!
And I'm really looking at the agar with antibiotics in the mix. Sounds like "how would it make it through the pressure cooking?".
Edited by happy4nic8r, 18 February 2015 - 07:00 PM.
Posted 18 February 2015 - 07:03 PM
Antibiotics are horrible imo , which ones do you refer to? You know they are planning on banning them in soaps etc because they don't work and breed super-viruses ...
Curious what antibiotic substances you plan on using.
FDA proposes ban on antibacterial soaps
Antibiotic resistance: delaying the inevitable
Edited by hyphaenation, 18 February 2015 - 07:10 PM.
Posted 18 February 2015 - 08:30 PM
It was actually on the patent website for the Chanterelles patented spwan.
Again in the archives in Seeker2be's daily Log of the Aloha scholarship he attended.
They had a few hundred recipes for agar and among them were a dozen that included cillins, and cyclins.
I guess it's kind of the same sort of thing, counter=attack, pre=emptive strike,
I also saw it referred to in the United Nations report on world hunger and mushroom cultivation.
Like antibiotics aren't more expensive than food?
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Posted 18 February 2015 - 09:21 PM
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Posted 18 February 2015 - 09:25 PM
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Posted 18 February 2015 - 09:34 PM
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Posted 18 February 2015 - 09:50 PM
Posted 19 February 2015 - 08:30 PM
FME, when your "best bud" patents you idea, and makes a bundle of cash, you will really know how not funny that is.
Posted 19 February 2015 - 09:01 PM
Cycloheximide (Dr. Dan’s Favorite antibiotic)
Put 1 mL of 1% cycloheximide solution in through filtered syringe and into any 1L of media (malt and potato based recommended). To make 1% cycloheximide solution weight out 0.1 g of cycloheximide and bring solution up to 10 mL with water.
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Posted 20 February 2015 - 11:34 AM
Posted 20 February 2015 - 12:32 PM
I think it's sort of interesting that they put 2.5 times the amount of agars worth of antibiotics in what would be each batch.
The link didn't look like that in the agar recipe book, I'm guessing these are individual additives, not all at once.
Having an interesting time reading about the dangers of all those antibiotics you list above.
What dangers and to whom? It's not my list, by the way, from the archives.
Posted 24 February 2015 - 03:07 AM
In the wonderful world of weed growing... trich is good.... but there's another pesky mold... powdery mildew, that will overtake certain kush strains.
One way of getting rid of it organically is using baking soda. Think it's 4 teaspoons/gallon of water as a foliar spray. I'm pretty sure it has to do with PH.
I wonder what the PH tolerance of cube myc is? They use lime baths for certain edibles that raises the PH so high contams won't grow. Growing in places where people can't afford autoclaves/sterilizers. Next time you feel like going iron mike with somer trich... try baking soda ( acid i think) or a high base solution.
Just a thought.
Posted 24 February 2015 - 08:17 AM
I had a 6qt zombie tub that had some sort of green mold that I treated with just h2o2 3% and it kept kicking out fruit bodies and stopped showing SIGNS of contamination..
Posted 27 February 2015 - 05:51 PM
Imagine the millions you can make from a sure-fire provable way to kill trichoderma in substrates with 100% dependability. Congrats man... you got Stamets beat. Mushroom farmers worldwide will be singing your name.... one thing, where's the proof? Got a link?
Someone told me this
To grain... use around 1-5% by volume of pickling Lime, Calcium Hydroxide.
To you subs, add around 1-5% by volume of pickling lime, dol lime, gypsum, calcitic lime, crushed/powdered oyster shell,
whatever you have on hand really...
Your Trich mean green ventures will be over with for the most part ime...
Also, Penn State
A few fungi are serious threats to the mushroom crop. Most recently, a Trichoderma green mold epidemic swept through mushroom crops for 3 years causing major losses measured in the $100 to $200 million. Improved sanitation, hygiene, fly control and the application of benomyl to mushroom spawn before the spawn is mixed with the substrate have reduced the continuing threat to 5% to 10% crop loss. When Trichloderma green mold was at its worst, crop yields were reduced an average of 35%, with a range of 20% to 80%. The other benzimidazole with a mushroom label, thiabendazole, is a much less effective spawn treatment. Another persistent disease is caused by a species of the genus Verticillium. This disease, known as Verticillium spot and dry bubble, routinely causes crop losses between 15% and 60%, and at times 100%. Sanitation and farm hygiene are important management strategies for this disease, as is an effective insect management program. Fungicides registered for use on this disease include: chlorothalonil, benomyl and thiabendazole. These fungicides can reduce disease incidence between 25 and 55%, but this level of disease control is difficult to see in a crop. The most widely used fungicide used for control of Verticillium is chlorothalonil. Casing surface weed molds are mostly cosmetic, although their occurrence can be impeded by the use of any of the three labeled fungicides. http://www.ipmcenter...pamushrooms.pdf