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Contaminations or Mold? Pictures included!!


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#1 lilantlife

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Posted 18 February 2015 - 11:01 PM

hello Mycotopians,

 

it is I, the learned one!!

 

I've inoculated 3 jars on the 28th of January, and was it an enjoyable experience. *ba dum tss*

 

here they are. my Odisha India triplets.

001_1.jpg

 

 

beautiful, arent they?

 

009.jpg

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how about now?

 

I've taken a peek under the 3 lids and only one seems to be doing OK for itself. These two, however, has me alarmed. The lids weren't tampered with until today, and underneath were these.

 

Will the mycelium do epic battle and fend off the invaders? Find out on the next post, on Mycotopia Z!!

 

also, newly molted german cockroach for your viewing pleasure.

 

008_1.jpg

 

looking forward to your replies


Edited by lilantlife, 18 February 2015 - 11:03 PM.


#2 hyphaenation

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Posted 18 February 2015 - 11:10 PM

Trichoderma... the green meanie. Time to chuck them before they contam your whole house. When you see green, its sporing and spores are now all over you and your camera. Just my opinion. See what others say. I'm sure someone will be by shortly to say you should let it go and see if the cube myc battles and kills the green trich. Good luck.
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#3 Saphroziac

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Posted 18 February 2015 - 11:27 PM

I've seen trich grow on the contamination barrier but I promptly washed it out in in the sink and still used the cake in my bulk sub. Risky but the sub is fruiting now so.



#4 hyphaenation

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Posted 18 February 2015 - 11:30 PM

Second pic, the white mycelium looks like trichoderma myc...

Edited by hyphaenation, 18 February 2015 - 11:31 PM.

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#5 Fletch

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Posted 18 February 2015 - 11:38 PM

Can not definitively say that is trich. If those are pf style jars, it looks like you have two errors. One being whole rice, and the other being a dry barrier layer which was not filled nearly high enough to the top of the jar.

#6 hyphaenation

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Posted 18 February 2015 - 11:42 PM

If that green is not trich , what do you think it might possibly be Fletch ?


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#7 lilantlife

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Posted 18 February 2015 - 11:50 PM

Hey fellas. snapped a couple of more photos for the defense, before i throw the case out the door. (heheh)

002.jpg

003.jpg

 

Its brown rice, and i suppose whole rice is the more technical term for this? sounds alot more reasonable to me too. also, this strain seems to be a very picky eater. it is leaving a few shards of vermiculite uncontested.

 

any chance of keeping this couple of jars to fruiting?

 

and fletch, yeah i didn't give the barrier much vermiculite as it should be. it'll be corrected next time around


Edited by lilantlife, 18 February 2015 - 11:52 PM.

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#8 Fletch

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Posted 18 February 2015 - 11:53 PM

It's really hard to tell. I can not see it terribly well. It does some that some uncolonized, somewhat wet, whole rice kernels are taking the color on as well. Could be aspergillus. Could be trich.

#9 hyphaenation

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Posted 18 February 2015 - 11:54 PM

If nothing else you will have very colourful jars. 


Edited by hyphaenation, 18 February 2015 - 11:55 PM.

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#10 Fletch

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Posted 18 February 2015 - 11:58 PM

New pictures, have same level of clarity to me. I mean you may have a chance to fruit, but if you did not use ground rice I doubt this cake will hold well. U need to cap the jars, and return to them in a couple days. Return and take pics again, and we should be able to guide you more perfectly on your quest. We should be able to ascertain what is happening more fully after, the added colonization period. Do nothing until, these days pass. Do not open the lids. Do not birth. Just wait.

#11 lilantlife

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Posted 18 February 2015 - 11:59 PM

uber paranoid now, ehh... gonna disinfect my room. what caused this mess, anyway? if you dont mind me asking

 

to the above, will do.

 

 

also, happy lunar new years everyone.


Edited by lilantlife, 19 February 2015 - 12:02 AM.

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#12 Fletch

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Posted 19 February 2015 - 12:02 AM

Well, it's not really a big deal. You have learned many things. You have learned that you need to grind the rice. You need to fill the dry barrier to the top of the jar or it doesn't really work. Additionally, pf jars are typically smaller than pints to increase your chances of success by faster colonization of smaller substrate.

But you have not yet failed. You just need to breathe and wait.

#13 hyphaenation

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Posted 19 February 2015 - 12:09 AM

Your pics link to photobucket, which links to your name... just sayin. Do what thou wilt.
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#14 lilantlife

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Posted 19 February 2015 - 12:10 AM

oh. jesus christ i forgot. thanks for the heads' up.

 

also, NOO!! xD


Edited by lilantlife, 19 February 2015 - 12:11 AM.


#15 wharfrat

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Posted 19 February 2015 - 12:17 AM

trich^ no question.. it's in the jars there are toast


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#16 Fletch

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Posted 19 February 2015 - 12:29 AM

How did it happen could be a long story, which I think will be best left for two or so days from now.  Only once we can positively id what it is, can we tell you the answer.  It still may be nothing, although unlikely.

 

For a general answer, lack of sterilization or aseptic technique, inadequate verm layer, even slightly slowing the process though pint jars, contaminated syringes or spores, using whole rice, opening lids, etc are all possible factors.

 

As far as disinfecting the entire room, it is absolutely unnecessary.  Disinfectants are for surfaces, mostly strictly.  Lysol for instance will need to be sprayed over the surface so that it is completely wet and not just a light mist quickly.  The disinfectant kills some microbes on contact, more after 5 minutes, and eventually reached that 99.9% value at about 15 minutes.  However, most things we need to worry about in mycology, are gone by the midway point.  Hardy viruses, for the most part, are what take the 15 minutes. But anyhow, that's just a bit of info for you moving forward. 

 

Likely the possible contamination issue you have will not benefit from disinfecting.  Trich and aspergillus are both spread in open air.  You could Lysol all the live long day and they will still be there.  Other contamination issues are inside the grains that may or may not have been properly sterilized.  Either way, what is there (or isn't there), is there and there is very little you can actively do now.  If it turns out to be these contaminations, I would instruct you to toss.  It a great way to approach contams as a new grower instead of trying this and that while you spread spores around the whole house.  If it is still iffy as to if this is a contam, I would probably say to try and birth.  Only time will tell.

 

GL


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#17 MLBjammer

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Posted 19 February 2015 - 04:16 AM

It's usually from a dirty syringe in my experiences.  No big deal.  Just start over.

 

Good luck.


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#18 BlackPeter17

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Posted 19 February 2015 - 05:40 AM

toss em!


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#19 Microbe

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Posted 19 February 2015 - 06:30 AM

Seriously? Everyone's just going to ignore the cockroach?

Your jars need tossed enough about that. I do however want to talk about the cockroach. Cockroaches make my skin crawl as they do to many others and kudos to you for picking it up as if it were a pet. Seems how you dont have a issue with handling them, why not try to utilize them to your advantage. I say them because im certain you know that you have more then one. I learned some interesting facts about cockroaches once owning and operating a pest control business. The interesting one is that their central nervous systems produce natural antibodies that have been proven to destroy some really nasty bacteria.Research has shown that in a lab cockroach brains destroyed a few bacteria including some very dangerous and even deadly to humans such as as methicillin-resistant Staphylococcus aureus (MRSA) and toxic strains of Escherichia coli that cause meningitis.

Studies also show that each molecule seems to be diffrent and potentially has evolved to target individual bacterium. The potential is something that is not very clear as of yet but if you think about the cockroaches habitat or the natural habitat of insects in general as other insects have been discovered to have these strong antibodies, it makes sense that these nasty critters naturally produce these antibodies in order to survive in despicable conditions.

Conventional microbes extracted from soils then cultured and grown in labs are becoming less effective each year at fighting off some of the deadliest bacteria on our planet and the cockroach might be the future of our front line defense against these deadly bacteria.

Now my point is try to figure out away to utilize the cockroach on a agar plate when you come face to face with a bacteria contaminated culture. How would you do this? I would start with research on the.molecular make up of the antibody and find out its solubility and melting point. If the melting point exceeds the temps of a autoclave or PC then continue. Once you determine its solubility you can determine the appropriate liquid(s) to use and perform a crude kitchen chemistry extraction process and try to isolate the molecule(s) then add this solution to your agar and BAM you advanced home cultivation into the future and can start culturing mycelium in the emphasis Sunoco Gas Station restrooms . Maybe take it a few steps forward and refine it to a powder and market it as a nutrient agar additive.

This wont help with tryc but i felt impelled to share this info about cockroaches and cockroaches will one day be saving human lives i guarantee you that. Now this doesnt mean that i will share my dwelling with them, as if i did it wouldnt be very long because i will either eradicate them or burn my house down and move. Good luck on your myco adventures and remember.............


2015-02-19-06-12-39-109091511.jpeg

Edited by Microbe77, 19 February 2015 - 06:38 AM.

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#20 oneeye1

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Posted 19 February 2015 - 11:30 AM

can doing a brf tek wrong completely mess your house/dwelling up for future work.

 






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