Using tryptophan to increase alkaloids
Posted 15 March 2015 - 08:09 PM
Today I spawned two bags of two quarts colonized popcorn each into 6 quarts of coir/straw/verm substrate at a 40/40/20 ratio respectively. I added 1900mg tryptophan powder to both of the six quart batches before pasteurization. I have two more bags inoculated from the same two clones that are about ten days behind. These will be the control batches. They both contain two quarts of popcorn and I will spawn them into an identical mix.
I'm going to test potency in two ways. One way will be via methanol extraction of like amounts of fruits. Secondly will be a blind test. I will have a friend grind up dry fruits and capsule them up. Then we will administer the capsules to four people. I will not know which is which. Exactly the same dose will be given to the group. Two weeks later he will give the people the other batch from that strain. Compare trips and see if either one felt stronger to anyone.
As I get more strains I will try this more. Also with varying amounts of tryptophan.
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Posted 15 March 2015 - 10:05 PM
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Posted 15 March 2015 - 10:24 PM
I have heard of this long ago and would like to know more!
Posted 16 March 2015 - 02:28 AM
Further experiments would tell you more if you used the same clone/strain, but varied the amount of potential "precursor".
Looking awesome so far!
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Posted 16 March 2015 - 08:17 AM
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Posted 16 March 2015 - 10:26 AM
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Posted 16 March 2015 - 10:46 AM
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Posted 16 March 2015 - 10:53 AM
Then get busy son! Seriously, good stuff. I'll be reading this thread.
Posted 16 March 2015 - 03:33 PM
This is cool. Keep at it!
Edit: actually you still have lots of this culture. When you spawn those jars, put a tiny piece of myc or a single kernel of popcorn on a plate/jar to make a master culture. Or spawn another jar g2g to make a master jar for another run of jars with the same culture.
Edited by TurkeyRanch, 16 March 2015 - 03:58 PM.
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Posted 16 March 2015 - 06:09 PM
Posted 18 March 2015 - 07:27 AM
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Posted 19 March 2015 - 03:20 AM
I'm going to follow this- I have full myc'd out jars of popcorn- wondering if my next thing-
My 100% O2 pressuring the housing unit to 3lbs- it was awesome- I grew such amazing shrooms- then when leaked water all over my carpet under the wall and into her side of the closet- I turned the mad scientist down a bit- but mushrooms did better in the 02 box than ever- but it is too expensive and could be potential hazardous to everything and one. So- skip the pressurized O2 grow- it didn't work well- it was kinda weird too- like mushroom farts when the valve would let air out-
Thank goodness for the Internet-
So the O2 worked maybe because I was paying so close attention all the time. That was before I took 7 grams. Now I am a more patient man. Thanks hindsight!
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Posted 06 April 2015 - 07:59 PM
Both Argentina tubs went bad. This strain seems to have almost no resistance to contams. To date only one tub has made it to fruition. On a brighter side both burma tubs are doing great, I've begun fanning them. The enhanced tub is starting to knot up already.
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Posted 06 April 2015 - 11:49 PM
I think TurkeyRanch has it ...
Results are questionable unless you're working with at least a single culture - otherwise, it's all too subjective and dependent on variables.
It's been an ongoing debate whether or not this would work - It'd be cool if someone really hit the research, but - unfortunately - it's often just easier to eat more shrooms :)
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Posted 08 April 2015 - 01:52 PM
Both of the lc's that I used were used up between these two batches. I'll be cloning from each tub and repeating the experiment with double the tryptophan. I should also be getting some prints in the mail but I dont really want to try this with ms unless the results are fantastic, so it will be a little while before I can try it with those. If anyone else out there wants to test this too, I'd like to hear the results. Tryptophan is readily available at your local health food store, it is a kinda pricey item at $26 but worth it in the name of science.
The cost is why I bailed on similar experiments a long time ago. I'd even been using quinoa as a supplemental grain (doesn't work as 100% quinoa) as it's the grain with the highest level of tryptophan (among grains).
IME, this is one of those "theory vs. practice" dilemmas. The old cliche' goes "In theory, there should be no difference between theory and practice. But in practice, there's a difference."
It may well have been boosting psilocin/psilocybin content but the subjective difference (compared to a non-supplemented sub) was too subtle to be definitive. Then in a thread about it posted at the time the discussion veered toward a consensus that the best way to maximize psilocybin production within a given space is to switch to woodlovers or Pan Cyans.
One thing to keep in mind is that all we can do is allow a strain to express its maximum genetic potential, which has an upper limit. Adding more special nutrients than it can use means the surplus won't be used, and tryptophan is expensive!
During that discussion a member (I think it was Waylitjim) reminded us that the world's most potent psilocybe grows on plain ol' wood (though there's some speculation that P. subaeruginosa may be stronger in some cases). IMO a cost/benefit analysis would conclude that focusing on growing woodlovers would be the most efficient approach to getting as much magic as possible in the smallest amount of space and with the cheapest substrate material.
This is a handy chart of psilocin, psilocybin, and baeocystin content of 12 different species which illustrates relative potencies pretty well: https://www.erowid.o...oms_info4.shtml
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