Now I can say it is not worth it.
Tryptophan supplement does not increase psilocin or psilocybin levels since it would only result in 4-htp and for any reason, maybe a time factor, it would not reach the methyltransferase to psilocin.
(5-ho-)tryptamine would be (re)hydroxylated at the 4 position and there seems to be enouth time for a methyltransferase of small amounts to an extra psilocin content of very very little more than normal. But the costs relativ to the outcome is definitively not worth that action since there are better ways to increase growth or biosynthesis.
If 5-htp would lead to an extra amount of psilocin/psilocybin, then the effect woukd be minimal and the effort not worth it.
In tests, see Gold's article "the mushroom entheogen part 3a: nutritional influences on growth and biosynthesis of psilocybin", many carbohydrates and nitrates were tested for the ability to increase growth and/or psilocybin production. The mushrooms need for nutrition is a relation of 9/1 for carbohydrates/nitrogen.
in short the best carbon source for increasing mycelium growth is maltose.
For psilocin production the best source for increasing the alkaloid content is dextrose (d-glucose). One teaspoon dextrose per jar increases that content by 25%! More dextrose makes it very difficult to shake the jar, because of the sirup effect.
There are nitrogen sources that inhibit growth and biosynthesis. An excess of tryptophan results in production of toxic ingredients inhibiting mycelia growth, so you do not want to use it or even soy beans.
Since the mushroom needs carbohydrates for its biosynthesis and since glycin is an amino acid the helps the carbon takeup in cells, and since rye has no glycin, glycin is a very good candidate for nitrogen source. Tests has shown that a mix of glycin/succinic acid 9/1 helps increasing the total alkoloid content.
Last not least the mushroom needs trace elements vitamines and minerals. Tests has shown that a combination of carbohydrate-nitrogen-traceelement supplement is more effective that any if these in its own. Complex media always improves the benefit from each single nutritional source.
Yeast extract is a good candidate as a vitamin b source but i think we may experimenting with others...
Edited by sesamstrasse1312, 19 February 2016 - 08:20 AM.