While I know it is much better to use Agar to innoculate LC's I simply have not gotten to my Agar yet. I finally got my LME so I can make a superiour Agar and hope to get to that soon. I also have my stack of sterile petri dishes... (little ones... like 2 inches - they used to be 4 inches back in the day!)
So I got 4 LC jars, two are filled with white filiments and little wads of mycellium, the other 2 are clear - I guess they didnt get anything. Hope its cubes in the two, could be anything if my print was not as clean as I had hoped. At any rate, I am going to innoculate some cakes with these to see what comes up.
While those cakes get going I'm going to make up some agar plates and see about making some clean LC's and innoculate lots more cakes! Teff Cakes!
Here is the recipie ( a lot less complex than my last run)
10 cups verm
2 cups Brown Rice Flour
1 cups Teff Flour
1 TBSP Gypsum (which is standard black-board chalk ground to powder. You can use plaster of paris, but you MUST mix it with water, let it harden, and then regrind it because POP is not gypsum until it is rehydrated.)
3 cups of H2O2 (that's right, I hydrate with Hydrogen peroxide. This kills all bacterial and Trich, The PC step will drive off the extra oxygen atom leaving just the water).
No coffee, honey or corn meal this time. There will be plenty of sugar in the LC mix I squirt in anyway.
When I get lots of LC that I am confident with, I will then explore using Coir tubs and other stuff. I shall endovor to make lots of prints to to give away!
Edited by SteampunkScientist, 28 August 2015 - 10:19 AM.