Paradox
©
Fisana

Jump to content


Photo
* * * * * 3 votes

Peacefrog's agar style and recipes


  • Please log in to reply
68 replies to this topic

#1 peacefrog

peacefrog

    Peaceful Frog

  • Moderator
  • 2,326 posts

Awards Bar:

Posted 31 October 2015 - 10:30 AM

I was asked to put up an agar prep post, so I went through some of my old pics and took a few more for this thread.

I used to sterilize in bulk and pour into petri dishes for many years, but I became tired of purchasing sleeves of plastic pre-sterilized petri dishes that could only be used once. They do make glass petri's that can be used more than once, but since I had so many jars, I never wanted to spend the money on them. And since I am just a hobbyist, I don't need 20 plates for my grows. That being said, my style and recipes are geared for smaller grows and allows me to make just enough agar for the size of my grows. My recipes make about 6-8 4 oz jelly jars of agar.

I mix up my dry ingredients and add to jars of water, stir then sterilize. This is easier and cleaner IMO, due to the fact that you do not need the added step of pouring. Note: I use food grade agar agar purchased from health food stores so the amount in the following recipes may need to be tweaked if using commercial grade agar agar. But a general rule is 20 grams of agar agar for 1 liter of water.

Recipe 1 PDA (powdered):
Ingredients.JPG
5 grams (2 tablespoon+ 1 teaspoon) of agar agar
5 grams (1 tablespoon+ 1 teaspoon) of instant potatoes
2 grams (1 teaspoon) of dextrose sugar (also known as corn sugar and can be found at a brewery store). You can also use 1/4 teaspoon of honey or karo distributed as evenly as possible into each jar, but make sure the water is piping hot. This will allow the honey or syrup to dissolve properly.

Recipe 2 PDA (potato water):
Cut up approximately 300 grams (3 small potatoes or 1 large baking potato) of potatoes and place in a pot with 4 cups of water and boil lightly for 45 minutes. Then take out the potatoes and eat or throw away. Finally, pour into a jar or something similar to use as your water. This can be frozen and taken out later for making more plates:
Raw pot..JPG Pot. in water.JPG Pot. Water.JPG
The same recipe as above can be used.

Recipe 3 MEA:
5 grams (1 teaspoon) of light malt extract (also can be found at a brewery store)
4 grams (a heaping teaspoon) of agar agar.
MEA.JPG

Recipe 4 Cornmeal Agar:
5 grams (1 and 1/4 teaspoon) of corn meal (yellow or white)
4 grams (a heaping teaspoon) of agar agar
Optional: 1 gram of dextrose or 1/8 teaspoon of honey or karo
Corn meal.JPG

Recipe 5 BRF Agar:
5 grams (1 + 1/2 teaspoon) of BRF ground fresh (preferred) or packaged flour
5 grams (2 tablespoon+ 1 teaspoon) agar agar
Optional: 2 grams of dextrose or 1/4 teaspoon of honey or karo
Whole BRF.JPG BRF.JPG

I mix all dry ingredients, add 15 ccs of water, add a heaping teaspoon of agar mix for each jar. I then stir and sterilize at 15 pci for 30 minutes. I personally do not use holes or micro tape on my jars, but a lot of growers like using either/or. So use whatever you feel comfortable with.
Jars ready dry ing..JPG wood water in jars.JPG ready for pc.JPG

Once sterilized, I take all jars out of the PC and place them in a sterile tub or similar and allow to cool and solidify. If condensation becomes an issue, wait about 2-4 hours and invert the jars and allow them to continue to cool until you inoculate. Upon opening the jar for inoculation, remove the lid upside down and with a quick motion, shake off all condensation from the lid onto the surface of whatever you using, before returning the jars up right. Then you should be able to inoculate/streak the plate normally.

Test tubes:
Any recipe will work for long term storage in slants. I fill my tubes about 1/2-3/4 full of water, add a teaspoon of dry agar mix using a paper funnel, shake very well, wrap in foil, and sterilize at the above psi and time. After it is done, it is important to get it out of the PC before it solidifies. You want to get it solidified on an angle. I use a computer stand for my angle, but you can use a block of wood or something similar. My advice would be to pour the above amount of water in one and test different angles to give you the right thing to use. And of course, you want the slants to cool and solidify in as sterile of an environment as possible.
Slants 1.jpg Slants 2.jpg Slants 3.jpg

Edited by peacefrog, 31 October 2015 - 01:40 PM.

  • MLBjammer, whitethumb, Hash_Man and 12 others like this

#2 psycheholic

psycheholic

    S-W-I-M Team

  • OG VIP
  • 2,726 posts

Awards Bar:

Posted 31 October 2015 - 11:06 AM

Nice write up PF. Couple questions, can you substitute lme for when recipe requires dextrose? How tightly do you screw the rings down on those inverted lids? Maybe loose in the PC, then tighten when cooled?

I really dig the corn meal recipe, great for folks who use grains that contain corn. That should train the myc to produce the enzymes needed to digest corn thus speeding up colonization.

As far as making a template for test tubes. I would use a Kregs jig and drill holes into a 2x4, then use those holes as a pilot holes to drill to desired size. Kinda like making a rack. Just my initial thoughts. You could probably free hand it, but I am perfectionist.

Edited by psycheholic, 31 October 2015 - 11:11 AM.

  • MLBjammer, peacefrog and Lakegal7 like this

#3 peacefrog

peacefrog

    Peaceful Frog

  • Moderator
  • 2,326 posts

Awards Bar:

Posted 31 October 2015 - 12:12 PM

psycheholic,

Thank you. Sorry I'm not sure what you mean by "lme", you must forgive me for my ignorance. And the bands are treated exactly like a cake, loose the whole time.

I like your idea for a rack. Nice! I'm pretty good with tools and handy, but never tried that. Thanks for the advice! And it is a great addition to this post for people wanting to explore agar.

I'm not a corn guy myself, only because I have never tried it actually, but that is a good point. I never thought about it. Out of many recipes I have tried, these are my favorite and so easy to make.

Edited by peacefrog, 31 October 2015 - 12:17 PM.

  • MLBjammer and psycheholic like this

#4 MLBjammer

MLBjammer

    Shaman Pouch!

  • Moderator
  • 9,424 posts

Donator


Awards Bar:

Posted 31 October 2015 - 12:58 PM

LME=liquid malt extract.


  • psycheholic and peacefrog like this

#5 happy4nic8r

happy4nic8r

    cyans rule!!

  • OG VIP
  • 3,584 posts

Donator


Awards Bar:

Posted 31 October 2015 - 01:17 PM

Also does 1 cc equal 1 ml, (and weigh a gram of H2O)?

 

Nice write up, I started doing that dry mix and not heating it up first, letting the pc do that.

 

good stuff.


Edited by happy4nic8r, 31 October 2015 - 02:21 PM.

  • Heirloom and peacefrog like this

#6 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 11,796 posts

Awards Bar:

Posted 31 October 2015 - 01:25 PM

Peacefrog: Did you mean 20mg of agar for 1 liter of water? Just checking.

 

Also does 1 cc equal 1 ml, (and weigh a gram of H2O)?

yeah i do 5 grams agar, 5 grams nutrition,  to 500ml h2o

 

I also hope that @seeker2be @microbe77 chime in too!


Edited by catattack, 31 October 2015 - 01:47 PM.

  • Tenderfoot and peacefrog like this

#7 peacefrog

peacefrog

    Peaceful Frog

  • Moderator
  • 2,326 posts

Awards Bar:

Posted 31 October 2015 - 01:46 PM

Peacefrog: Did you mean 20mg of agar for 1 liter of water? Just checking.
 
Also does 1 cc equal 1 ml, (and weigh a gram of H2O)?


Thanks, happy. You are right. Once again my proffreading skills need some work lol. I changed the post 1 liter of water for 20 grams Of agar agar. And yes I cc is the same as 1 ml. My bad.

LME=liquid malt extract.


Thanks, jammer. Now that I know what that acronym means, I'm sure it would work. I have never tried it in place of dextrose, but have tried liquid malt with good results. I just like the dryed variety better, as it tends to last longer.
  • MLBjammer likes this

#8 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 11,796 posts

Awards Bar:

Posted 31 October 2015 - 01:51 PM

there's also every agar recipe known to man here:

 

https://mycotopia.ne...ship-month-log/


  • Heirloom , peacefrog, Opalguy and 2 others like this

#9 peacefrog

peacefrog

    Peaceful Frog

  • Moderator
  • 2,326 posts

Awards Bar:

Posted 31 October 2015 - 02:13 PM

Thanks nice link, cat.
  • MLBjammer and CatsAndBats like this

#10 psycheholic

psycheholic

    S-W-I-M Team

  • OG VIP
  • 2,726 posts

Awards Bar:

Posted 31 October 2015 - 03:09 PM

Well I don't use corn per say, but there is cracked corn in the ACE brand WBS I use. Probably be best to make a tea of what ever grain and final fruiting substrate I will use, and use that tea in place of my water, throw in a little lme (which does come in a dry powder btw) that ought to be nice to work with. I just started playing with agar a few months ago, so I have much to learn. Lme alone, makes a bomb ass LC too. I use 1 gm per 600 ml and the LC just explodes, but I have to use w/in a couple months due to the lack of nutrients for long term storage.
  • peacefrog likes this

#11 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 11,796 posts

Awards Bar:

Posted 31 October 2015 - 03:34 PM

Well I don't use corn per say, but there is cracked corn in the ACE brand WBS I use. Probably be best to make a tea of what ever grain and final fruiting substrate I will use, and use that tea in place of my water, throw in a little lme (which does come in a dry powder btw) that ought to be nice to work with. I just started playing with agar a few months ago, so I have much to learn. Lme alone, makes a bomb ass LC too. I use 1 gm per 600 ml and the LC just explodes, but I have to use w/in a couple months due to the lack of nutrients for long term storage.

actually, it's my understanding that you can put any mycelium in distilled, non-nutritious, sterile h2o and keep in the refrigerator for a very long time. I learned how to do it at a mushroom course that I took with Peter McCoy as the teacher.


  • psycheholic, peacefrog and Traderkain like this

#12 peacefrog

peacefrog

    Peaceful Frog

  • Moderator
  • 2,326 posts

Awards Bar:

Posted 31 October 2015 - 03:57 PM

Cat, I have read about that. I have never tried it though. It is interesting.
  • CatsAndBats and Justintime like this

#13 psycheholic

psycheholic

    S-W-I-M Team

  • OG VIP
  • 2,726 posts

Awards Bar:

Posted 31 October 2015 - 07:43 PM

Right on, a vaccutainer would probably work great for that. Unfortunately my myco fridge had a freon leak and I had to abandon it. So I don't have the luxury of refridgeration any more, which is why I worded my post the way I did.
  • CatsAndBats likes this

#14 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 11,796 posts

Awards Bar:

Posted 31 October 2015 - 08:07 PM

I just started a batch of 'spore germination' agar off of the aloha thread:

 

10g agar

10g cornmeal

500ml h2o

a little squeeze of dextrose (recipe calls for 7g, I eyeballed it)

 

a little powdered oats, coir, and eggshell (all in small amounts to 'train' the myc to build/secrete the enzymes used to digest it's future food)

blue food color stain for identification of said agar


  • psycheholic and Traderkain like this

#15 coorsmikey

coorsmikey

    Hippiecritical

  • App Administrator
  • 6,686 posts

Donator


Awards Bar:

Posted 31 October 2015 - 09:52 PM

The activated charcoal seem to work well for germination as well, I didn't re read the the thread but if I remember correctly its Aloha's R-7 agar recipe.
  • psycheholic and peacefrog like this

#16 MLBjammer

MLBjammer

    Shaman Pouch!

  • Moderator
  • 9,424 posts

Donator


Awards Bar:

Posted 01 November 2015 - 02:28 PM

 

Well I don't use corn per say, but there is cracked corn in the ACE brand WBS I use. Probably be best to make a tea of what ever grain and final fruiting substrate I will use, and use that tea in place of my water, throw in a little lme (which does come in a dry powder btw) that ought to be nice to work with. I just started playing with agar a few months ago, so I have much to learn. Lme alone, makes a bomb ass LC too. I use 1 gm per 600 ml and the LC just explodes, but I have to use w/in a couple months due to the lack of nutrients for long term storage.

actually, it's my understanding that you can put any mycelium in distilled, non-nutritious, sterile h2o and keep in the refrigerator for a very long time. I learned how to do it at a mushroom course that I took with Peter McCoy as the teacher.

 

I have tried that, and it did not work for me.  The mycelium died every time.


  • psycheholic and CatsAndBats like this

#17 psycheholic

psycheholic

    S-W-I-M Team

  • OG VIP
  • 2,726 posts

Awards Bar:

Posted 01 November 2015 - 02:42 PM

Perhaps just a touch of nutrition would help, MLB.



#18 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 11,796 posts

Awards Bar:

Posted 01 November 2015 - 03:39 PM

 

 

Well I don't use corn per say, but there is cracked corn in the ACE brand WBS I use. Probably be best to make a tea of what ever grain and final fruiting substrate I will use, and use that tea in place of my water, throw in a little lme (which does come in a dry powder btw) that ought to be nice to work with. I just started playing with agar a few months ago, so I have much to learn. Lme alone, makes a bomb ass LC too. I use 1 gm per 600 ml and the LC just explodes, but I have to use w/in a couple months due to the lack of nutrients for long term storage.

actually, it's my understanding that you can put any mycelium in distilled, non-nutritious, sterile h2o and keep in the refrigerator for a very long time. I learned how to do it at a mushroom course that I took with Peter McCoy as the teacher.

 

I have tried that, and it did not work for me.  The mycelium died every time.

 

 

will you expand mlb? I think it's supposed to 'die' to be reanimated once it encounters nutrition again..


  • MLBjammer likes this

#19 MLBjammer

MLBjammer

    Shaman Pouch!

  • Moderator
  • 9,424 posts

Donator


Awards Bar:

Posted 02 November 2015 - 04:45 AM

I was advised that mycelium/tissue water could survive a while many years ago, but every time I tried storing and reviving it, it contaminated.  So I just used it immediately upon making it.

 

Other growers still swear it will last for months, but that wasn't my experience, and I know that the tissue/mycelium was initially clean.

 

It was just an experiment.


  • CatsAndBats likes this

#20 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 11,796 posts

Awards Bar:

Posted 02 November 2015 - 07:15 AM

yeah, the guy I leaned it from uses a lot of LC's, when I use LC's I end up making penicillin ;)


Edited by catattack, 02 November 2015 - 07:15 AM.





Like Mycotopia? Become a member today!