I make 1 liter, tap water
20g malt dextrose
1g soy peptone optional
1g yeast optional
mix it with hot water in the blender and pour jars, petrie dishes, remainder into clear glass bottles
put in pc for 30 - 40 mins, when cold tighten jar lids, wrap parafilm around plates, cork bottles.
All go into the glove box, except bottles which go in fridge.
I have also used the bottles stored in fridge by microwaving until melted and hot, and poured more plates with that.
Sometimes it works. Not 100% works, but 50 - 75% will grow without issue.
Sometimes I have had it not harden at all, sometimes it hardens like a brick, these out of the same bottle.
It is such a random success thing with me that I really just stick to other things that work, but i still keep trying.
I found that adding the ingredients slowly in the blender on slow with hot *(not boiling) water made a good clear mix.
Trying to boil it on the stove was time consuming, messy and had clumps that were hard to mix out.
Is there something I'm doing wrong here?