398 replies to this topic

[peacefrog]

Awesome cat! I liked that one so much I changed it to my avatar. Thanks for finding and posting that one, my friend.

[CatsAndBats]

So, should I make grain spawn out of the pan myc? Or just inoculate lil tupperwares dirt cakes with agar? Every time that I try trays, I fuck them up. I'm thinking the manure supplemented PF cakes done chronic style might give me enough 'neglect' but still have them get enough FAE. I should probably noc up some grain I think.

[peacefrog]

You can do either IME, cat. My little invitro style experiment grew just fine with them. But I still prefer grain to poo in trays or a mono. Perhaps you should do both and see which one you like the best.

What has happened in the past with your pan trays?

[CatsAndBats]

You can do either IME, cat. My little invitro style experiment grew just fine with them. But I still prefer grain to poo in trays or a mono. Perhaps you should do both and see which one you like the best.

What has happened in the past with your pan trays?

 

What happened is I was overly ambitious and barely had a couple grows under my belt. 

 

Now I have a much broader understanding of how mushrooms grow than when I first tried. I'm confident that I'll be successful.

[peacefrog]

I am confident for you. Looking forward to seeing your results.

[CatsAndBats]

THANKS! Guess I'll start a thread. That's how I keep on stuff.

Edited by catattack, 02 September 2016 - 08:03 PM.

[Microbe]

probably here.

WT is right but you can take anything between 6 and 12 imo because the first trait i chase is speed.

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[GlitchInSpace]

i like to go for speed but i also like to select for uniform growth.

[Microbe]

i like to go for speed but i also like to select for uniform growth.

As do i bit that comes second. It requires selection after selection and obtaining multiple cultures that eventually be put to the test then start the process of elimination. A skilled agar tech does mix random and selective transfers but at the end it is all selective.

Process of elimination i say! I have aborted many cultures literally (down the garbage disposal) and im not sure how i feel about that, perhaps they have a chance to colonize and fruit from the sewer walls i dont know but i absolutely have no time for working cultures to be unproductive. I spend a lot of time on the front end to deal will shenanigans from a unproductive cultures on the back end ;)

[CatsAndBats]

 

i like to go for speed but i also like to select for uniform growth.

As do i bit that comes second. It requires selection after selection and obtaining multiple cultures that eventually be put to the test then start the process of elimination. A skilled agar tech does mix random and selective transfers but at the end it is all selective.

Process of elimination i say! I have aborted many cultures literally (down the garbage disposal) and im not sure how i feel about that, perhaps they have a chance to colonize and fruit from the sewer walls i dont know but i absolutely have no time for working cultures to be unproductive. I spend a lot of time on the front end to deal will shenanigans from a unproductive cultures on the back end ;)

 

 

 

One of the benefits of doing them in jars is one can add water and shake the shit out of them and use the myc water in the garden or potted plants. 

 

 

Edited by catattack, 04 September 2016 - 05:38 PM.

[CatsAndBats]

 

 

Had no idea where to put this, but it's grain that was contaminated, re-PCd and is being consumed by aa+

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Edited by catattack, 04 September 2016 - 06:26 PM.

[mycolangelo.4HO]

Had no idea where to put this, but it's grain that was contaminated, re-PCd and is being consumed by aa+

That's cool, I had thought about trying that before but never actually did it. I mean if it works why not do some g2g transfers to RE pressure cooked (contaminated) grain. That picture of pan trop on agar, is that MS inoculation? I have some on agar that I took to grains but I think there's a bacterial contam, it smells right but lots of metabolites, would it be best to start over from spore or try to clean the culture? Just moved and I've gotta shrink my growspace so I'm planning on trying to get some pan trops, cambos, mexicana jalisco (fruits&sclerotia), basically going for more exotics, just gotta learn how they like to grow.

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[CatsAndBats]

I do not suggest that any grower re-PC any contaminated jars for the purpose of reinoculating. I do however suggest that growers re-PC contaminated jars to kill contaminants before opening them to throw out or put in the compost pile.

 

I however am probably over mindful of unwanted spores and endospores. 

 

@mycolangelo, which photo? @whitethumb has one and I have several. Mine are multispore germinated on agar.

Edited by catattack, 05 September 2016 - 12:39 PM.

[GlitchInSpace]

i toss anything that might be suspect. don't even pc it. just toss and clean with bleach. grain is cheap. I also make a liquid culture of everything i do agar work on.

that panaeolus tropicalis started from a print. i just made some more isolations from that donor plate.

[CatsAndBats]

 don't even pc it. just toss and clean with bleach. 

 

I am only suggesting re-PCing so that one is not covering one's clothing and room with mold spores. 

 

That said, I do all sorts of things that I wouldn't suggest other people do in their own grows though. I'll inoculate virtually anything. I had a jar of BPK that destroyed a re-PC'd trich jar, and in theory, that culture was better 'prepared' to exposure to live/dormant trich spores. 

Edited by catattack, 05 September 2016 - 03:17 PM.

[mycolangelo.4HO]

Sorry I think it probably was Whitethumbs' pic, was wondering what would explain my pan Tropicalis myc to look so different. I mean my gf had a very good description she says it looks like the skin of an orange, very strange texture, I was expecting something that looked like pan cyan myc, I'm thinking bacteria. When I put it on grains it looks very different from the cambos I've got going and every jar of trops develops a lot of moisture

What I'm hoping is pan trop on rye


And this was my first attempt MS to rye grain, I was a bit concerned so I posted pics on another website and I was told to toss it immediately, so I opened it and could smell a very distinct mushroom smell though different from cubes, so I thought this was just how the myc looked, but now I'm thinking maybe bacteria, any thoughts?



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[CatsAndBats]

 

 

Sooooo, it's been awhile. I am not going to lie, I've let my agar work fall WAY behind. As usual though, the mycelium has surprised me again. Pictured is self isolating rhizomorphic growth of a malabar culture that has actually run over already colonized agar. I will transfer this to continue the isolation.

 

Not pictured, the Hawaiian pans that went from "milked" agar to oats .

 

 

Holy shit! This thread is one year old this month!

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Edited by catattack, 01 November 2016 - 09:04 PM.

[CatsAndBats]

So today's recipe is:

 

12g telephone brand agar

10g potato flake

1g activated charcoal

~.5g catattives (eggshell, straw, BRF, sucrose, corn starch, coir, soil, morning glory straw)

600ml tap water (chlorine, flouride and trace potassium salts [I'm assuming] will challenge the myc or at least trigger extra polysaccharides and will "arm" the myc to consume it in the future if applicable)

 

Simmering on low to distribute said nutes throughout the agar. Here's what 1g of activated charcoal does color wise:

 

 

Here's a post that I often double check for recipes:

https://mycotopia.ne...-log/?p=1198427

Edited by catattack, 02 November 2016 - 08:58 AM.

[mycolangelo.4HO]

I'm sure you've gone over it before, I remember reading part of this thread a while ago, is the activated charcoal for color? Making it easier to see the mycelium vs contaminants, I'm guessing also making it easier to see sectors. I thought I read that somewhere before, but also read that introducing something into the agar will help the mycelium develop the best enzymes(?), to break that down better. I started adding water that had horse manure boiled and strained out (no clumps at all), I haven't done a side by side so IDK if it works better but I've been sticking with it. I've been wondering about using water from grains, or straw or both. The manure water makes the agar a bit darker (which I kinda liked). Do you add actual eggshells and things or is it such a small amount it isn't a problem, wasn't sure if you do
.5gram of each of the cattatives, or .5gram of them combined?

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[CatsAndBats]

I'm sure you've gone over it before, I remember reading part of this thread a while ago, is the activated charcoal for color? Making it easier to see the mycelium vs contaminants, I'm guessing also making it easier to see sectors. I thought I read that somewhere before, but also read that introducing something into the agar will help the mycelium develop the best enzymes(?), to break that down better. I started adding water that had horse manure boiled and strained out (no clumps at all), I haven't done a side by side so IDK if it works better but I've been sticking with it. I've been wondering about using water from grains, or straw or both. The manure water makes the agar a bit darker (which I kinda liked). Do you add actual eggshells and things or is it such a small amount it isn't a problem, wasn't sure if you do
.5gram of each of the cattatives, or .5gram of them combined?

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Activated charcoal aids in spore germination and I almost exclusively use agar for germination. One can use more for staining purposes as I learned at the radical mycology convergence (RMC, mad props to Peter McCoy). I also learned at the same workshop (cordyceps militaris, mad props to mycosymbiotics) that one can use bio-char/activated charcoal in lieu of vermiculite, however I haven't tried it yet.

 

Here's TCO's take (more props): https://mycotopia.ne...rcoal/?p=993548

 

Regarding my 'catattives', it was .5g total. I add them at the earliest stage to help the myc develop the alkaloids (polysaccharides)  to digest future food.  I add them at later stages for the calcium boost (and microbe77's carbon boost).

Edited by catattack, 02 November 2016 - 12:16 PM.