this is pretty cool, building a frankenstein of a culture it seems that you are doing.
whenever I've messed with training a strain I usually want it to be able to process or adapt quickly to an already pre-known environment.
for example if I know I'm spawning to wbs or a heavily supplemented substrate with coffee I might add soakwater from the grain and a bit of coffee to the agar I'm putting the culture in.
This way when it get's transferred out to the real deal it'll be much better equipped to consume the substrate and increase chances of success and as well as speed along colonization times.
I've also wanted to begin experimenting with training oyster myc to process cigarette buds, something that has just been starting to garner some attention for it's bioremediation impact.
A quote from http://www.no-smoke....ore.php?id=731
"Cigarette butts are the most commonly discarded piece of waste worldwide. It is estimated that 1.69 BILLION pounds of butts wind up as toxic trash each year, creating an enormous environmental, health, and economic burden.
Contrary to popular belief, cigarette butts are NOT biodegradable! Cigarette butt waste is a huge environmental issue, with a global impact -- it is both unsightly and unhealthy." and "Cigarette filters are made from cellulose acetate, a plastic that can break into smaller pieces, but will never biodegrade or disappear. "
I think it's completely reasonable to say that we can train mycelium to process almost any material out there with varying degrees of success. This project with the discarded filters will require a culture aggressive enough to deal with contaminations usually present in the filters as well as having the capacity to degrade the cellulose acetate found in them. Because putting the dirty filters through a sterilization process before the introduction to the mycelium seems like counter productive and inefficient way to get the job done, I'd like it if I could train the organism to process them raw and unsterile.
I think it'll be interesting to see what I found out from this project and possibly some of your experience in "training" myc could be useful to increase the chances of success.
I appreciate you sharing your efforts and insights!
Edited by truMushrooms, 23 March 2016 - 09:36 AM.