Invisibilitysyndrome - is dead-on correct in thinking that these grains can be used outdoors.
In the past, a certain "other" project became contaminated with bacteria. An outdoor bed was made and the contaminated spawn was introduced. One month later, the bed produced fungi. Bacteria cannot stand fresh air and competition.
Here's the account:
Liquid Culture was used to start 3 quart jars of RGS.
Once colonized, the RGS jars were added to sterilized spawnbags which contained 3 jars' worth of seed.
In all, once inoculated, the bags contained the equivalent of 4 total quart jars - per bag. The spawn size is not the problem - nor was sterilization method. After 8 years of intensive study, this is definitely not my first fungus rodeo.
I can't stress this enough.......
Bacteria - cannot be killed. It creates endospores which cannot be destroyed by high heat. I posted a scientific study once where this hypothesis was studied and supported in detail. I will scrounge for the article and see if I can find it again.
The live mycelium began rampant colonization.
The increase in bio-activity raised the temperatures above 80*F due to the fact that I had just placed these bags all in a single cardboard box for darknes. The bags were nested together - snuggled.
Bacterial endospores began to awaken and increase the heat - generating more bacteria
Exactly 24 hours after spawning - I went to admire the culture progress and noticed that the bags (while looking perfect) were "fever-ish". They were warmer than my body temperature.
I knew what was going-on and immediately broke up the colonized grain mass to reduce the temperatures. I then laid out the bags with the grains spread as thinly as possible and tried to see if they would recover. Several random checks showed that the bacteria had become well-established (sour apple smell issuing from the filter patch) but the mycelium was still there.
Since these King Oyster mushrooms were so aggressive - colonizing in the refrigerator - I decided to go ahead and stick them on the composted straw bales of my garden. This was not the original plan but I thought they stood a better chance if placed on a more level playing field (compost as opposed to experimental substrates).
Upon spawning and watering, the healthy smell of mycelium was present.
I then cased the bales with some worm-laden compost to help preserve moisture and provide some living inoculators to spread the mycelium.
The environment now waters with an automatic drip system. It's out of my hands. Now I just wait and see what comes.
It's just discouraging to fall victim when "I knew better" - shameful.
I had declared (to my family) that this years' garden was going to be my thesis. ( I never went to college )
Strangely, it still is. Fruiting King Oysters from contaminated grains - outdoors - is still a demonstration of observed science.
As for the timing........
I went to work on this in January so I would be sure and still have a little cold weather with which to get ahead.
Over the years, I've learned that "food" mushrooms will run in cold conditions. Cold temperatures suppress other organisms like mold and bacteria.
I've also learned that beds spawned after late April or early May fall prey to local predators (molds, bugs) who awaken from their winter rest and eat everything living thing in sight. Let's just say that I almost shed a tear between bouts of kicking myself.
I'm trying to learn to sustain myself from my backyard. I want to be able to stop going to the "grocery store" as a practice-run for what our sociopathic world leadership has planned for us. Better yet, I intend to increase the quality of the foods I eat in the hopes of improving my mental coping skills and problem-solving abilities in the lead-up to this inevitable disaster. Buckle-up campers, your leadership does not have your best interests at heart.
I'm just glad to get the practice part out of the way while there still is a grocery store from which to purchase "shit" to eat. It certainly beats starving to death simply because I fucked-up my garden.
Best to learn this stuff now - before our lives depend upon it.
Best to archive this knowledge now and pass it to our friends/neighbors/loved ones. Starving times are coming again someday - our leadership veritably assures us of this fact. It's scary to know that many folks don't know where food comes from........much less how to raise it successfully. Fast-forward one or two more generations and revisit this thought. Very scary indeed.
"Peaches come from a can. They were put there by a man, in a factory downtown." - The Presidents of the United States of America.