My very first try was on the 17th and failed to start. I tried again on the 22nd and that jar grew cobweb and STANK!
Today, I tried two different methods: PF Tek in one and agar in the other. The PF tek jar has 1cc total of cube spores in three spots below the verm. layer. My agar did not solidify but is quite thick. No measuring, just a dash of this and pinch of that. So... I thought I’d try and make a LC out of it. I dropped in a small swab with P. Semi. spores from a print.
I threw out the BRF with cobweb mold but I’m holding out a little longer for my first pair of jars. Almost two weeks and no signs of anything growing. One is cubes from a syringe and the other from the print. I know the dry spores will take some time but the syringe?...
Surely something will take! I really would like a flush this summer of something. Hopefully, the Lib Caps will have enough so I can make a new print or two. What characteristics should I look for in a good cap that is likely to drop spores. I’ll not pick those so they can open.
You really have to follow a proper recipe for agar because it needs to solidify properly since the main reason to use agar is that the myc will grow on the agar surface in a flat, 2 D "disc" because the agar is solid. If it's not solidified the spores will fall down under the surface making it impossible to see contams. That is the main reason we use agar, to see the contams so we can transfer any clean areas of myc away to a new clean agar plate. Here's some links for great agar recipes, "fastfred's cookbook", here is "Peacefrog's agar style and recipe",
here's a link for a great recipe book pdf download I got from a post from coorsMikey.
Here is my own write up for my method/tek and recipe, which is probably the easiest and least likely method to contam on you because it is a "no-pour" method, skipping the step of pouring after sterilization, cutting down the risk of picking up a contam from the air while pouring, which has to be done in front of a flow hood or at least in a still air box. I would definitely recommend the "no-pour" tek for any first timer, assuming you are, maybe your not, it's a great method either way. Good luck!
Edited by Billcoz, 28 April 2019 - 09:52 PM.